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pENTR L1-vas2-Cre-L4

Plasmid

#62301

Purpose

Cre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoter

Depositor

Eric Marois

Article

Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

Insert

Cre recombinase under control of vasa promoter and terminator

Use

Tags

HA and SV40 NLS

Expression

Insect

Mutation

Promoter

vasa

Available since

March 23, 2015

Availability

Academic Institutions and Nonprofits only

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pGL4.10-VEGFprom(-950 to -700)

Plasmid

#66133

Purpose

Luciferase-based reporter for VEGF promoter region (-950 to -700 bp)

Depositor

David Mu

Article

Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

Insert

Vascular Endothelial Growth Factor-A promoter region (VEGFA Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

VEGF -950 to -700

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Tbxa2r

Plasmid

#170304

Purpose

A knock-out vector for the mouse Tbxa2r

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse Tbxa2r gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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PliaI

Plasmid

#55177

Purpose

Bacitracin-inducible promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

liaI promoter

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

liaI

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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TRE-eGFP-F1C

Plasmid

#211457

Purpose

Dox inducible delivery of eGFP fused to a flotillin-1 palmitoylation blocking peptide (F1C)

Depositor

Linda DeGraffenried

Article

McClellan et al Oncogene. 2024 Mar;43(14):1063-1074. doi

Insert

Flotillin-1-palmitoylation blocking peptide (FLOT1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

None

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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TRE-eGFP-F1A

Plasmid

#211458

Purpose

Dox inducible delivery of eGFP fused to a flotillin-1 palmitoylation mutant control peptide (F1A)

Depositor

Linda DeGraffenried

Article

McClellan et al Oncogene. 2024 Mar;43(14):1063-1074. doi

Insert

Flotillin-1-palmitoylation mutant control peptide (FLOT1 Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

C34A

Promoter

Available since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221-rap1-K9R

Plasmid

#84527

Purpose

This plasmid can be used as a donor for subcloning into Gateway destination vectors.

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Gateway donor plasmid

Tags

Expression

Mutation

K(43,61,69,117,240,246,527,582,651)R

Promoter

none

Available since

Jan. 17, 2017

Availability

Academic Institutions and Nonprofits only
pcDNA3-RIPK3[S199D][S227D]-FLAG

Plasmid

#199324

Purpose

double mutated phosphorylation site (kinase acitivity and MLKL interaction active)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 and 227 to Aspartic Acid

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pRP(Exp)-CMV> ORF_924bp (Azgp1):T2A:dTomato:IRES:Puro

Plasmid

#110830

Purpose

Expresses zinc alpha-2 glycoprotein (ZAG) and dTomato (dT) reporter where both sequences are separated by a T2A self-cleaving peptide sequence as a result, the dT is not fused with the ZAG protein.

Depositor

Ande Satyanarayana

Article

Elattar et al FASEB J. 2018 Mar 23:fj201701465RR. doi:

Insert

AZGP1 (Azgp1 Mouse)

Use

Non-viral gene expression vector

Tags

T2A and dTomato

Expression

Mutation

Promoter

CMV

Available since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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Bin1 delta N-BAR

Plasmid

#168282

Purpose

Express N-BAR-deleted Bin1 mutant (aa 268-476)

Depositor

Cheng-han Yu

Article

Cao et al Commun Biol. 2020 Mar 13;3(1):117. doi:

Insert

Bin1 (Bin1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

aa 268-476

Promoter

Available since

May 18, 2021

Availability

Academic Institutions and Nonprofits only

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pIK208

Plasmid

#65630

Purpose

sgRNA targeting dpy-10 (Arribere et al., 2014) expressed from a C. elegans U6 promoter (Friedland et al., 2013), in a "flipped and extended" sgRNA backbone (Chen et al., 2013)"

Depositor

Iskra Katic

Article

Katic et al G3 (Bethesda). 2015 Jun 3. pii: g3.115.0

Insert

U6 promoter::dpy-10 sgRNA with a "flipped and extended" sgRNA backbone

Use

CRISPR

Tags

Expression

Worm

Mutation

Promoter

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2puro-Ptgs2_B6

Plasmid

#170300

Purpose

A knock-out vector for the mouse Ptgs2 in C57BL6

Depositor

Michiyuki Matsuda

Article

Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

Insert

A gRNA targeting the mouse tyrosinase gene.

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 8, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-26 (GB1001)

Plasmid

#68208

Purpose

Provides the A. thaliana U6-26 RNA polIII promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

AtU6-26 promoter

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

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BAT-gal

Plasmid

#20889

Depositor

Stefano Piccolo

Article

Maretto et al Proc Natl Acad Sci U S A. 2003 Mar 18. 1

Insert

7XTCF binding sites, Siamois minimal promoter, NLS-lacz

Use

Mouse Targeting

Tags

Expression

Mammalian

Mutation

7 multimerized TCF-binding elements cloned upstre…

Promoter

Available since

May 7, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA3-RIPK3[S199A][S227A]-FLAG

Plasmid

#199323

Purpose

double mutated phosphorylation site (kinase acitivity and MLKL interaction inhibited)

Depositor

Adrian Press

Article

Hoff et al Cell Death Dis. 2023 Apr 18;14(4):275. d

Insert

Receptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 199 and 227 to Alanine

Promoter

Available since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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HMGA2(3'UTR DEL)+GFP

Plasmid

#25406

Depositor

Sean Morrison

Article

Nishino et al Cell. 2008 Oct 17. 135(2):227-39.

Insert

high mobility group AT-Hook 2 (Hmga2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

3" UTR is absent

Promoter

Available since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pLV-rtTA-zeo

Plasmid

#183233

Purpose

Lentiviral vector expressing the rtTa gene

Depositor

Jorge Ferrer

Article

Beucher et al Nat Cell Biol. 2022 Oct 6. pii: 10.1038/

Insert

rtTA-T2A-Zeo

Use

Lentiviral

Tags

Expression

Mutation

Promoter

UBC

Available since

Oct. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac1 PIK3R1-Ni

Plasmid

#17744

Depositor

Bert Vogelstein

Article

Samuels et al Cancer Cell. 2005 Jun . 7(6):561-73.

Insert

PIK3R1-Ni (PIK3R1 Human)

Use

Tags

Expression

Insect

Mutation

Partial cDNA

Promoter

Available since

April 14, 2008

Availability

Academic Institutions and Nonprofits only

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PliaG

Plasmid

#55174

Purpose

Constitutive promoter

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

LiaG promoter

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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pSLQ1371-sgSTAT3-1

Plasmid

#121425

Purpose

sgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

Depositor

Stanley Qi, Ovijit Chaudhuri

Article

Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

Insert

sgSTAT3-1

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

mouse U6

Available since

April 22, 2019

Availability

Academic Institutions and Nonprofits only

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