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Plasmid#100731PurposeTo express a clathrin "hook" in mammalian cells to hot-wire endocytosis (negative control)DepositorInsertAP2A1 (Ap2a1 Mouse)
UseTagsFKBP and GFPExpressionMammalianMutationLong isoformPromoterCMVAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-AlstR-IRES2-EGFP
Plasmid#14895DepositorInsertAlstR (AstA-R1 Fly)
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMay 11, 2007AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-OsTIR1(WT)
Plasmid#140729PurposeOsTIR1(WT)-P2A-mAID-EGFP-NESDepositorInsertOsTIR1(WT)-P2A-mAID-EGFP-NES
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
EYFP-NLS-actin-K68/284R
Plasmid#60616PurposeEncodes YFP and NLS tagged beta-actin with sumoylation resistant mutationsDepositorInsertBeta-actin (ACTB Human)
UseTagsEYFP and NLSExpressionMammalianMutationK68R, 284RPromoterAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-HseIF4E
Plasmid#79437PurposeExpresses EGFP-tagged HseIF4E in Mammalian cellsDepositorInsertHseIF4E (EIF4E Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBabe-puro GSK3 beta (S9A)
Plasmid#14128DepositorInsertGSK3 beta (S9A) (GSK3B Human)
UseRetroviralTagsV5 tagExpressionMammalianMutationS9A. Mutant version of GSK3 that lacks the inhibi…PromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pLXSN IkB alpha M
Plasmid#12330DepositorInsertIkB alpha M (Nfkbia Mouse)
UseRetroviralTagsExpressionMammalianMutationDominant-negative mutant. Many signal transductio…PromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Sst22-GFP
Plasmid#172312PurposeSST interneuron-restricted gene regulatory element GRE22, to drive GFP expression in SST+ interneuronsDepositorInsertSst22
UseAAVTagsExpressionMutationPromoterpB-GlobinAvailable sinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA-HBXIP
Plasmid#42328DepositorInsertHBXIP (LAMTOR5 Human)
UseTagsHAExpressionMammalianMutationPromoterCMV, SP6Available sinceMarch 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAWPSG-Po-nCas9-BE
Plasmid#195740PurposeExpress APOVEC1-nCas9(D10A)-UGI using phenol inducible system in Methanotroph or E. coliDepositorArticleInsertDi-Methyl Phenol Regulatory protein / APOBEC1-nCas9(D10A)-UGI
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPo (phoenol-inducible promoter)Available sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_His-ZZ-TEV-Lis1
Plasmid#132539PurposePlasmid for insect cell expression of human full-length LIS1 with an N-terminal His-ZZ-LTLT tagDepositorInsertLis1 (PAFAH1B1 Human)
UseTags8x His, Linker-TEV-linker_TEV, and ZZExpressionInsectMutationPromoterAvailable sinceOct. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSKA413
Plasmid#80381PurposeConstitutive CcaS and CcaR with gfpmut3 under the cpcG2Δ59 promoter.DepositorInsertsccaS (complement)
ccaR (complement)
gfpmut3
UseSynthetic Biology; OptogeneticsTagsFLAG tagExpressionBacterialMutationPromoterccaR and cpcG2Δ59Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd (no NLS) g4+g10+g18 (FWA)
Plasmid#117168PurposeCRISPR Cas9 SunTag system to target NtDRMcd (without an NLS) to the FWA locus with three guide RNAsDepositorInsertg18_U6_g10_U6_g4_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[S227D]-FLAG
Plasmid#199320Purposemutated phosphorylation site (MLKL interaction permanent active)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged Serine 227 to Aspartic AcidPromoterAvailable sinceMay 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RIPK3[S227A]-FLAG
Plasmid#199318Purposemutated phosphorylation site (MLKL interaction inhibited)DepositorInsertReceptor-interacting serine/threonine-protein kinase 3 (RIPK3 Human)
UseTagsFLAGExpressionMammalianMutationchanged Serine 227 to AlaninePromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
COC053
Plasmid#209131PurposeEnzymatic biotinylation of proteins at a specific lysine residue in a recognition sequence. This has multiple applications, especially in the study of protein-protein interactions.DepositorInsertEnzyme BirA (birA E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV_pSyn_Mito-LOV-Turbo-V5
Plasmid#199669Purposeexpresses Mito-LOV-Turbo-V5 in neurons, AAV vectorDepositorInsertLOV-Turbo
UseAAVTagsCOX4I1 mito targeting sequence and V5ExpressionMutationPromoterSynapsinAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
LZF1826 pAAV_hSyn-DiO-SomArchon-EGFP-P2A-stGtACR2
Plasmid#135412Purposesimultaneous optical recording and manipulation of electrical activity in neuronsDepositorInsertSomArchon-EGFP-P2A-stGtACR2
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
SMC2 CRISPR
Plasmid#140649PurposeSMC2 tagging CRISPRDepositorInsertSMC2 (SMC2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA
Plasmid#100279PurposeIn vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutantDepositorInsertKdm6b (Kdm6b Mouse)
UseTagsFlag epitope tagExpressionMammalianMutationMutated catalytic domain (1025aa-End) (please see…PromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only