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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14895 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV-Syn-IRES2-EGFP
- Backbone size w/o insert (bp) 6700
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameAlstR
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Alt nameallatostatin receptor
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SpeciesD. melanogaster (fly)
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Insert Size (bp)1200
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Entrez GeneAstA-R1 (a.k.a. Dmel_CG2872, ASR, AST-R, AlstR, AstA R1, AstAR1, CG2872, D.AlstR1, DAR-1, DAR1, DGR, Dar-1, Dmel\CG2872, EG:121E7.2, GR)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer pBS-KS (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid features: Synapsin promoter 208-680, AlstR
1352-2536, IRES2 2570-3154, EGFP
3158-3895.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-Syn-AlstR-IRES2-EGFP was a gift from Edward Callaway (Addgene plasmid # 14895 ; http://n2t.net/addgene:14895 ; RRID:Addgene_14895) -
For your References section:
Selective and quickly reversible inactivation of mammalian neurons in vivo using the Drosophila allatostatin receptor. Tan EM, Yamaguchi Y, Horwitz GD, Gosgnach S, Lein ES, Goulding M, Albright TD, Callaway EM. Neuron. 2006 Jul 20. 51(2):157-70. 10.1016/j.neuron.2006.06.018 PubMed 16846851