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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 14128 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepBabe-puro
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Backbone manufacturerAvailable at Addgene (#1764)
- Backbone size w/o insert (bp) 5169
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Vector typeMammalian Expression, Retroviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGSK3 beta (S9A)
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Alt nameGSK3b
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Alt nameGSK3beta
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SpeciesH. sapiens (human)
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Insert Size (bp)1300
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MutationS9A. Mutant version of GSK3 that lacks the inhibitory phosphorylation site.
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Entrez GeneGSK3B
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Tag
/ Fusion Protein
- V5 tag (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site SalI (not destroyed)
- 5′ sequencing primer pBabe 5' (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pBabe-puro-GSK3β-S9A was generated by standard site-directed mutagenesis from a cDNA clone of human GSK3β.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBabe-puro GSK3 beta (S9A) was a gift from Brendan Manning (Addgene plasmid # 14128 ; http://n2t.net/addgene:14128 ; RRID:Addgene_14128) -
For your References section:
S6K1 regulates GSK3 under conditions of mTOR-dependent feedback inhibition of Akt. Zhang HH, Lipovsky AI, Dibble CC, Sahin M, Manning BD. Mol Cell. 2006 Oct 20. 24(2):185-97. 10.1016/j.molcel.2006.09.019 PubMed 17052453