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Plasmid#183087Purpose2A peptide P2A cloned in yeast MoClo entry vector (pYTK001) for use as the second 2A peptide in tri-cistronic and third 2A peptide in quad-cistronic construct assemblyDepositorInsertP2A
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
FnR8-10
Plasmid#182569PurposeHuman fibronectin fragment, type-III repeats 8-10, R1379A mutantDepositorInsertHuman fibronectin fragment, type-III repeats 8-10
UseTagsHis and TEV cleavage site after His tagExpressionBacterialMutationR1379APromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-66%GC-40bp
Plasmid#169200PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert66%GC-40bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-75%GC-20bp
Plasmid#169201PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert75%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-75%GC-40bp
Plasmid#169202PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert75%GC-40bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-100%GC-20bp
Plasmid#169203PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert100%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-Left-half-120bp
Plasmid#169204PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-Left-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-Right-half-120bp
Plasmid#169205PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-Right-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50-0-50%GC-Left-half-120bp
Plasmid#169206PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50-0-50%GC-Left-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50-0-50%GC-Right-half-120bp
Plasmid#169207Purpose50-0-50%GC-Right-half-120bp-hairpinDepositorInsert50-0-50%GC-Right-half-120bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-20bp
Plasmid#169196PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-40bp
Plasmid#169197PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert50%GC-40bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-50%GC-40bp-Revbindsite
Plasmid#169198PurposeForce-activating hairpin for 5'-to-3' helicaseDepositorInsert50%GC-40bp-hairpin-binding-site-reversed
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
hp-66%GC-20bp
Plasmid#169199PurposeForce-activating hairpin for 3'-to-5' helicaseDepositorInsert66%GC-20bp-hairpin
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCRISPR_full-site
Plasmid#186277PurposeReporter plasmid for full-site selection assayDepositorInsertThiomicrospira leader repeat and modified chloramphenicol selection cassette (removed RBS and start codon))
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMpGE010-sgRNA-Target1 (MpRTN1)
Plasmid#186295PurposeBinary vector for CRISPR/Cas9 (target 1: MpRTN1) in plants (for Agrobacterium-mediated genetic transformation)DepositorInsertsgRNA-Target1
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK2-mMOV10-3'UTR
Plasmid#178905Purposedual luminescence reporter to assess miRNA responsiveness in the 3'UTR of mouse Mov10 RNA helicaseDepositorInsert3'UTR mMov10 (Mov10 Mouse)
UseLuciferaseTagsExpressionMutationPromoterSV40 promoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-T11HA-hMOV10-WT
Plasmid#178907Purposeexpression of WT hMOV10DepositorInsertcDNA WT hMOV10 (MOV10 Human)
UseTagsT11 beta strand of GFP-HAExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9355(II-1_Markerfree_BackBone)
Plasmid#161597PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site II-1, (Chr II: 341622..341641)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB9356(IV-1_Markerfree_BackBone)
Plasmid#161598PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site IV-1, (Chr IV: 132921..132940)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only