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Plasmid#140760PurposeTargeting vector to Sox1 gene locus for induction of P2A-fused Cre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(WT) sequence.DepositorInsertCre
UseMouse TargetingTagsExpressionMutationPromoterEndogenous Sox1Available sinceJune 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Chronos(M140T)-mRuby2-ST
Plasmid#109129PurposeModified cation channelrhodopsin Chronos fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChronos(M140T)-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChroME(F243Y)-mRuby2-ST
Plasmid#109127PurposeModified cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChroME(F243Y)-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBW1997_pExpr-hU6-CRISPR-DECODER
Plasmid#87549PurposeExpresses NGN2, MIAT, ACTC1, and TTN gRNAs in response to combinations of Cre and Flp recombinasesDepositorInsertgRNAs targeting NGN2, MIAT, ACTC1, TTN
UseCRISPR, Cre/Lox, and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G12345 mutant
Plasmid#231920PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1134, N1158, N1173, N1194 by implementing Asn-to-Gln mutationDepositorInsertspike G12345 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1134Q, N1158Q, N1173Q, N1194QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G145 mutant
Plasmid#231918PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1173, N1194 by implementing Asn-to-Gln mutationDepositorInsertspike G145 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1173Q, N1194QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
SARS-CoV-2 spike G1345 mutant
Plasmid#231919PurposeFor expression of SARS-CoV-2 spike protein with glycan deletion at N1098, N1158, N1173, N1194 by implementing Asn-to-Gln mutationDepositorInsertspike G1345 (S SARS-CoV-2)
UseTagsFLAGExpressionMammalianMutationN1098Q, N1158Q, N1173Q, N1194QPromoterCMVAvailable sinceJan. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSCL758
Plasmid#219504PurposeExpress Eco3RT from a CAG promoter; also express an msr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequencesDepositorInsertmsr and HEK3, FANCF and EMX1 retron msd donor-gRNAs from a single H1 promoter, separated by tRNA-Cys-GCA sequences
UseTagsExpressionMammalianMutationPromoterH1Available sinceMay 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-mEGFP-HO-G
Plasmid#164163PurposeExpresses mEGFP (monomeric EGFP with the A206K mutation) under the control of CAG promoter, codon-optimized for Homo sapiens, basic vector, multiclonig site at C terminalDepositorInsertmEGFP A236K
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ePsuACR-mRuby2-ST
Plasmid#109133PurposeAnion channelrhodopsin PsuACR fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendritesDepositorInsertePsuACR-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-eiC++-mRuby2-ST
Plasmid#109132PurposeArtificial anion channelrhodopsin iC++ fused to ER export signal and fluorophore mRuby2 and targeted to the neuronal soma and proximal dendritesDepositorInserteiC++-mRuby2-ST
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pWN10042
Plasmid#89052PurposeGF-ori-FP plasmid for GFxFP assayDepositorInsertsEGFP part 1
EGFP part 2
UseCRISPRTagsExpressionMammalianMutationPromoterCAG and noneAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tg 2 kb Violet opsin GFP
Plasmid#72921Purposeviolet opsin promoter from zebra finch (Taeniopygia guttata) gDNA (nucleotides21,946 to 145, corresponding to chr1A_ran-dom:206453–208444 in taeGut1) driving GFPDepositorInsertviolet opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
Tg 2 kb Violet opsin DsRed
Plasmid#72920Purposeviolet opsin promoter from zebra finch (Taeniopygia guttata) gDNA (nucleotides21,946 to 145, corresponding to chr1A_ran-dom:206453–208444 in taeGut1) driving DsRedDepositorInsertviolet opsin promoter
UseTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWN_U6-B2M-miniGag-Cas9
Plasmid#228958PurposeminiEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)DepositorInsertminiGag-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJRH029
Plasmid#171060PurposePlasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in betweenDepositorInsertGag-spyCas9
UseCRISPR and LentiviralTags2x SV40 NLS and 3x FLAG tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWN_U6-TRAC-miniGag-Cas9
Plasmid#228959PurposeminiEDV production plasmid. Expresses the miniGag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter)DepositorInsertminiGag-Cas9
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEX-PK-hLC3
Plasmid#61458PurposeExpress pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagyDepositorInsertPK-hLC3 (MAP1LC3B Human, Fission Yeast)
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG dCas9-KRAB-2A-EGFP
Plasmid#92396PurposeCAG-driven ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. Contains 2A-EGFP reporter. For targeted enhancer inactivation in chicken embryos.DepositorInsertdCas9_KRAB_2A_EGFP
UseCRISPRTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
CANE-LV envelope
Plasmid#86666PurposepCAGGS/ES-M21EnvA-VSVg-WPRE, used to package pseudotyped lentivirusDepositorInsertCANE-LV envelope
UseLentiviral, Mouse Targeting, and Synthetic Biolog…TagsExpressionMammalianMutationPromoterCAGAvailable sinceFeb. 13, 2017AvailabilityAcademic Institutions and Nonprofits only