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pCIneo-lambdaN-HA-HsTNRC6Biso1_1250-1723-F1370A_K

Plasmid

#146745

Purpose

Mammalian Expression of HsTNRC6Biso1_1250-1723-F1370A

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6Biso1_1250-1723-F1370A (TNRC6B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6C_1248-1690-F1389A_K

Plasmid

#146746

Purpose

Mammalian Expression of HsTNRC6C_1248-1690-F1389A

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6C_1248-1690-F1389A (TNRC6C Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso1-siRNAres_I

Plasmid

#146543

Purpose

Mammalian Expression of HsTNRC6Biso1-siRNAres

Depositor

Elisa Izaurralde

Article

Huntzinger et al EMBO J. 2010 Dec 15;29(24):4146-60. doi:

Insert

HsTNRC6Biso1-siRNAres (TNRC6B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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K202R-short-UBASH3A-V5-6xHis

Plasmid

#195406

Purpose

Mammalian expression of human K202R short-form UBASH3A with V5-6xHis tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Tags

V5-6xHis

Expression

Mammalian

Mutation

K202R

Promoter

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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W279A-short-UBASH3A-V5-6xHis

Plasmid

#195409

Purpose

Mammalian expression of human W279A short-form UBASH3A with V5-6xHis tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Tags

V5-6xHis

Expression

Mammalian

Mutation

W279A

Promoter

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcna2

Plasmid

#192796

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcna2

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcna2

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcna6

Plasmid

#192797

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcna6

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcna6

Use

AAV and CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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V5-K202R-short-UBASH3A

Plasmid

#192084

Purpose

Mammalian expression of human K202R short-form UBASH3A with V5 tag

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Tags

V5

Expression

Mammalian

Mutation

K202R

Promoter

Available since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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phU6 NFATC2

Plasmid

#188708

Purpose

sgRNA plasmid encoding hU6 promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pmU6 NFATC2

Plasmid

#188709

Purpose

sgRNA plasmid encoding mU6 promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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p7SK NFATC2

Plasmid

#188710

Purpose

sgRNA plasmid encoding 7SK promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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phH1 NFATC2

Plasmid

#188711

Purpose

sgRNA plasmid encoding hH1 promoter driving an anti-NFATC2 sgRNA

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

NFATC2 sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-W279A-short-UBASH3A-Ntag

Plasmid

#192096

Purpose

Human W279A short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Tags

Expression

Mammalian

Mutation

W279A

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-K202R-short-UBASH3A-Ntag

Plasmid

#192094

Purpose

Human K202R short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Tags

Expression

Mammalian

Mutation

K202R

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-WT-short-UBASH3A-Ctag

Plasmid

#192099

Purpose

Human wild-type short-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-WT-short-UBASH3A-Ntag

Plasmid

#192100

Purpose

Human wild-type short-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

Depositor

Patrick Concannon

Article

Ge et al J Immunol. 2019 Dec 1;203(11):2827-2836.

Insert

UBASH3A (UBASH3A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMpGE_En03-sgRNA-Target1 (MpRTN1)

Plasmid

#186291

Purpose

Gateway entry vector for sgRNA (target 1: MpRTN1). Transient expression of sgRNA (Target 1: MpRTN1) for MpRTN1 in plant cells

Depositor

Yutaka Kodama

Article

Ishikawa et al Plant J. 2022 Apr 27. doi: 10.1111/tpj.1

Insert

sgRNA-Target1

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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PALI1_PIR_pFB1

Plasmid

#191249

Purpose

Expresses PALI1 from insect cells

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LCoR

Use

Tags

MBP

Expression

Insect

Mutation

NA

Promoter

Polyhedrin

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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LacZ_pHIVEGFP

Plasmid

#191251

Purpose

Expresses LacZ in mammalian cells (used as a control)

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

LacZ

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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E42_gControl_dTet_IRES_mTurquoise2

Plasmid

#189799

Purpose

Retroviral delivery of control guide RNA

Depositor

Ellen Rothenberg

Article

Hosokawa et al Immunity. 2018 Jun 19;48(6):1119-1134.e7

Insert

Luciferase gRNA

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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