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Plasmid#193317PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4FLAG N-terminal tagging
UseTags4FLAGExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGAL1-VP1 (LEU2)
Plasmid#182480PurposeYeast integrative plasmid for expressing deltaVP1, an NLS deletion mutant of Murine polyomavirus VP1 (GAL1 promoter). Contains leucine auxotrophic marker (K. lactis LEU2).DepositorInsertdeltaVP1
UseSynthetic Biology; Metabolic engineeringTagsExpressionYeastMutationPromoterGAL1Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAV105-PPAB1-GFP-Erk1R84S-1SIM
Plasmid#190253PurposeExpression Erk1-GFP-1SIM in yeast cellsDepositorInsertERK1R84S-SIM
UseTagsGFPExpressionYeastMutationERK1R84SPromoterAvailable sinceNov. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.027
Plasmid#184964PurposeExpress -Eco1 RT and ncRNA in yeastDepositorInsertEco1: RT and ncRNA(wt), a1/a2 length: 12
UseTagsExpressionYeastMutationhuman codon optimized RTPromoterGal7Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSCL.006
Plasmid#184970PurposeExpress -Eco1 RT in yeastDepositorInsertIntegrating inducible cassette: Eco1 RT
UseTagsSV40NLSExpressionYeastMutationhuman codon optimized RTPromoterGal1-10Available sinceNov. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV492
Plasmid#177700PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available sinceJuly 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnEA-NpM-Scp20_18-49-GB1_AF
Plasmid#148720PurposeBacterial Expression of Scp20_18-49DepositorInsertScp20_18-49 (CAF20 Budding Yeast)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV491
Plasmid#177699PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.DepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterRNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDIV488
Plasmid#177696PurposePlasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast speciesDepositorInsertsCas9
Nat
UseCRISPRTagsSV40ExpressionBacterial and YeastMutationPromoterTDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…Available sinceMarch 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTL316
Plasmid#168377PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os08g40130
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL360
Plasmid#168421PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os01g70240
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL362
Plasmid#168423PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os04g32860
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL374
Plasmid#168435PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os02g48170
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL387
Plasmid#168448PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os04g39370
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL394
Plasmid#168455PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os03g02070
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL393
Plasmid#168454PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertOsaHIP05 (Pi21)
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL382
Plasmid#168443PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os01g61070
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL380
Plasmid#168441PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os02g37180
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL366
Plasmid#168427PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os04g42350
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTL355
Plasmid#168416PurposepGBKT7 yeast two-hybrid plasmidDepositorInsertLOC_ Os06g30480
UseTagsExpressionYeastMutationPromoterAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only