Addgene

We narrowed to 16,240 results for: multi

Showing: 5101 - 5120 of 16240 results

Results per page:

Sort results by:

pDGE332

Plasmid

#153241

Purpose

M1 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pEU3-NII-GLICNot-C-His

Plasmid

#140187

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

6xHis and GST

Expression

Mutation

Promoter

T7

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pClathrin-miRFP670nano

Plasmid

#127430

Purpose

Clathrin labeling with near-infrared fluorescent protein miRFP670nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Commun. 2019 Jan 17;10(1):279. doi:

Insert

miRFP670nano-clathrin light chain

Use

Tags

miRFP670nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pYPQ134-tRNA2.0

Plasmid

#158396

Purpose

Golden Gate entry vector to express the 4th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Article

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Without promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLifeAct-miRFP713

Plasmid

#136562

Purpose

F-actin labeling with a near-infrared fluorescent protein miRFP713

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

LifeAct-miRFP713

Use

Tags

miRFP713

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pDGE333

Plasmid

#153242

Purpose

M2 shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ePPE-V223A

Plasmid

#205243

Purpose

For plant prime editing in wheat plants or monocotyledons protoplasts

Depositor

Zong Yuan

Article

Ni et al Genome Biol. 2023 Jun 29;24(1):156. doi:

Insert

SV40_NLS-nCas9-NC-MLV-SV40_NLS

Use

CRISPR

Tags

Expression

Plant

Mutation

Detailed in manuscript

Promoter

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBBK

Plasmid

#62670

Purpose

Vector for PhiC31o mRNA synthesis

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

PhiC31

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pvimentin-miRFP713

Plasmid

#136569

Purpose

Vimentin labeling with near-infrared fluorescent protein miRFP713

Depositor

Vladislav Verkhusha

Article

Matlashov et al Nat Commun. 2020 Jan 13;11(1):239. doi:

Insert

vimentin-miRFP713

Use

Tags

miRFP713

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG-eGtACR1-mRuby2-ST

Plasmid

#109136

Purpose

Anion channelrhodopsin GtACR1 fused to ER export signal, mRuby2 and targeted to the neuronal soma and proximal dendrites

Depositor

Hillel Adesnik

Article

Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

Insert

eGtACR1-mRuby2-ST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
p3XP3-WhiteA2

Plasmid

#165875

Purpose

Basic transgenesis 3xP3 white CDS physical eye marker cassette in Alpha2, lacks attB site

Depositor

Koen Venken

Article

Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

Insert

Eye Marker Cassette

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR221-CAG.Pro

Plasmid

#79490

Purpose

MultiSite Gateway entry clones, first fragment, (attL1, attR5) CAG promoter

Depositor

Peter Jon Nelson

Article

Jackel et al Sci Rep. 2016 Oct 3;6:34365. doi: 10.103

Insert

CAG Promoter

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

CAG Promoter

Available since

May 16, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEU3-NII-FLAGLICNot

Plasmid

#140184

Purpose

Used for producing recombinant proteins by wheat germ-based cell-free translation, contains T7 promoter, FLAG-tag and NotI restriction site

Depositor

Tamás Mészáros

Article

Nagy et al BMC Biotechnol. 2020 Mar 14;20(1):17. do

Type

Empty backbone

Use

Expression vector for a cell-free system

Tags

FLAG

Expression

Mutation

Promoter

T7

Available since

June 23, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pMN-018

Plasmid

#195422

Purpose

AAVS1 Integratable mCherry Reporter for synZiFTR ZF10

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF10 BS - minCMV - mCherry - RbGpA - bGHpA - pGK - PuroR - P2A - BFP - SV40pA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-hsRPA70 (MSW#473)

Plasmid

#208067

Purpose

Expression of GFP-tagged hsRPA1 in human cells

Depositor

Marc Wold

Article

Haring et al J Biol Chem. 2008 Jul 4;283(27):19095-11

Insert

RPA1 (RPA1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV/3' Box_(GLuc)_TOP1

Plasmid

#68434

Purpose

Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

Depositor

John Rinn

Article

Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

Insert

TOP1 construct

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pMB34

Plasmid

#122245

Purpose

Lentivirus delivery for stable expression of LbCas12a-VPR

Depositor

Darjus Tschaharganeh

Article

Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

Insert

mammalian codon-optimized Lachnospiraceae bacterium Cas12a fused to VPR activator

Use

Lentiviral

Tags

NLS and VPR activator

Expression

Mutation

Promoter

EFs

Available since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pEE393

Plasmid

#149283

Purpose

T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbPDS3

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbPDS3sgRNA_TrunFT:tNos

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

July 20, 2020

Availability

Academic Institutions and Nonprofits only
rTetR-GFP-VPR

Plasmid

#183918

Purpose

VPR activation domain coupled to reverse Tet repressor; labeled with GFP; binds tetO sites upon doxycycline addition

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

rTetR-GFP-VPR

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMA-SpCas9-g5

Plasmid

#80788

Purpose

BsaI-based cloning of SpCas9 gRNA guide sequence, position 5/15/25 in the array

Depositor

Yonglun Luo

Article

Vad-Nielsen et al Cell Mol Life Sci. 2016 May 13.

Insert

CRISPR gRNA expression cassette (for SpCas9)

Use

CRISPR

Tags

na

Expression

Mammalian

Mutation

Promoter

U6

Available since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge