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Plasmid#70223PurposeExpresses VMA intein with C-terminal portion of alpha-sarcinDepositorInsertalpha Sarcin (C-terminal portion)
UseTagsFKBP-rapamycin binding (FRB) domain of the mammal…ExpressionMammalianMutationChanged Asp 28 to Cys, deleted amino acids 1-27PromoterCMVAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEBB-II-I-GFP gamma isoform
Plasmid#22148DepositorInsertTFII-I (GTF2I Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_EBFP2
Plasmid#167857PurposePiggyBac compatible plasmid expressing spCas9DepositorInsertCas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2-krt4-GFP
Plasmid#97151Purposelabel apical epithelial cells in zebrafishDepositorInsertAcGFP
UseZebrafishTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBXNH3CA_MBP
Plasmid#132700Purposeexpresses Avi-tagged MBP in E.coli, to use as positive control in ELISADepositorInsertMaltose binding Protein
UseTagsAvi and HisExpressionBacterialMutationPromoterpBADAvailable sinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
RabV CVS-N2c(deltaG)-FlpO
Plasmid#73473PurposeExpresses FlpO for circuit mapping and genetic manipulationDepositorInsertFlpO
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRSV-p65S276A
Plasmid#106455PurposeNF-kB p65, kinase site 276 mutatedDepositorInsertNF-kB p65, kinase site 276 mutated (RELA Human)
UseLentiviralTagsExpressionMammalianMutationkinase site inactivatedPromoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAS-hActin-luc2
Plasmid#48081PurposeLuciferase Reporter with Gal4 DNA binding site upstream of hACTB promoterDepositorInsertLuciferase
UseTagsExpressionMammalianMutationremoved hsp70 promoter, inserted amplified 615 bp…PromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFGAMS-mMaple3
Plasmid#154912PurposeExpresses human PFAS-mMaple3 fusion in mammalian cellsDepositorInsertPFAS (PFAS Human)
UseTagsmMaple3ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQTEV-PTD004
Plasmid#34798DepositorInsertGTP-binding protein PTD004 (OLA1 Human)
UseTagsHis and TEVExpressionBacterialMutationPromoterAvailable sinceMarch 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioE
Plasmid#65351Purposesysthesis of vioE gene in the violacein pathwayDepositorInsertvioE
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEBG-TFII-I alpha
Plasmid#22154DepositorInsertTFII-I (GTF2I Human)
UseTagsGST and HisExpressionMammalianMutationPromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUC19-Split-Broccoli-Toehold-Switch
Plasmid#87316PurposeExpresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.DepositorInsertSplit-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterP70aAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pT7-Venus-Emi1 (914)
Plasmid#39854DepositorInsertEmi1 (FBXO5 Human)
UseIn vitro transcriptionTagsVenusExpressionMutationPromoterT7Available sinceOct. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNS3
Plasmid#131770PurposePADH1_FUSN_Fusion Red_Cry2Olig_TACT1 for Integration into δ-sitesDepositorInsertPADH1_FUSN_Fusion Red_Cry2Olig_TACT1
UseIntegration into δ-sitesTagsExpressionYeastMutationE490G to make Cry2 into Cry2OligPromoterPADH1Available sinceSept. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioB
Plasmid#65348Purposesysthesis of vioB gene in the violacein pathwayDepositorInsertvioB
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKMV-VioA
Plasmid#65347Purposesysthesis of vioA gene in the violacein pathwayDepositorInsertvioA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNS1
Plasmid#131769PurposePADH1_FUSN_Fusion Red_Cry2WT_TACT1 for Integration into δ-sitesDepositorInsertFUSN_Fusion Red_Cry2WT
UseIntegration into δ-sitesTagsExpressionYeastMutationPromoterPADH1Available sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLEX_970_puro_DEST_YFP
Plasmid#45295DepositorInsertYFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
CAG-RARa-VP16
Plasmid#185559PurposeUbiquitous expression of constitutively active Retinoic Acid Receptor alphaDepositorInsertRARa-VP16 (Rara Mouse)
UseTagsVP16ExpressionMammalianMutationPromoterCAGAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only