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Plasmid#101371PurposeName: pCBR-RBPjkΔA. RBPjk binding site A mutation in pCBR-K14 (pDD2005).DepositorInsertRBPjk binding site A mutation in pCBR-K14 (pDD2005).
UseLuciferaseTagsExpressionMutationRBPjk binding site A mutation in pCBR-K14 (pDD200…PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2029
Plasmid#101372PurposeName: pBluescript-LANApiΔTATA. LANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000).DepositorInsertLANApi TATA box mutation in pBluescript-LANApi,K14 (pDD2000).
UseUnspecifiedTagsExpressionMutationLANApi TATA box mutation in pBluescript-LANApi,K1…PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2030
Plasmid#101373PurposeName: pBluescript-RBPjkΔB. RBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).DepositorInsertRBPjk binding site B mutation in pBluescript-LANApi,K14 (pDD2000).
UseUnspecifiedTagsExpressionMutationRBPjk binding site B mutation in pBluescript-LANA…PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2031
Plasmid#101374PurposeName: pBluescript-RBPjkΔA. RBPjk binding site A mutation in pBluescript-LANApi,K14 (pDD2000).DepositorInsertRBPjk binding site A mutation in pBluescript-LANApi,K14 (pDD2000).
UseUnspecifiedTagsExpressionMutationRBPjk binding site A mutation in pBluescript-LANA…PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pWN10042
Plasmid#89052PurposeGF-ori-FP plasmid for GFxFP assayDepositorInsertsEGFP part 1
EGFP part 2
UseCRISPRTagsExpressionMammalianMutationPromoterCAG and noneAvailable sinceApril 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSI HA Nup53xl
Plasmid#86000Purposemammalian expression of HA tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
UseTagsHAExpressionMammalianMutationcodon optimzed for bacterial expressionPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSI myc Nup53xl
Plasmid#86001Purposemammalian expression of myc tagged Xenopus laevis Nup53DepositorInsertXenopus laevis Nup53 (nup35.L Frog)
UseTagsmycExpressionMammalianMutationcodon optimzed for bacterial expressionPromoterAvailable sinceMarch 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR-IAA10_no stop
Plasmid#83848PurposeGateway donor vector for IAA10 with no stop codonDepositorInsertindoleacetic acid-induced protein 10 (IAA10 Mustard Weed)
UseGateway donor vectorTagsExpressionMutationno stop codonPromoterAvailable sinceOct. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-myc-mCE NLS
Plasmid#82465PurposeExpresses myc tag and mRNA capping enzyme without Nuclear Localization Sequence (NLS) from C terminusDepositorInsertCapping Enzyme (Rngtt Mouse)
UseTagsMycExpressionMammalianMutationdeleted amino acids Nuclear Localization SignalPromoterCMVAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pICSL11062
Plasmid#68256PurposeLevel 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-2 in Brassica oleraceaDepositorInsertPromoter_AtU6-26 + sgRNA_BolC.GA4a-2
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL391
Plasmid#62821PurposeLim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::RBS (st7) hfq::mCherry::T1 terminator + ColE1DepositorInsertsgfp
mCherry
UseSynthetic BiologyTagshfp leader, ompC leader, and st7 RBSExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL518
Plasmid#62823PurposeLim Lab plasmid: AmpR + PLlacO-1::micC::mCherry::T1 terminator + PLtetO-1m9::OmpC::gfp::T1T2 terminator + ColE1DepositorInsertsmCherry
gfp
UseSynthetic BiologyTagsm9::ompC leader and micC leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2021
Plasmid#62833PurposeLim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::sodB::gfp::T1T2 terminator + ColE1DepositorInsertsT710 hfq leader
gfp
UseSynthetic BiologyTagsm9::sodB leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2026
Plasmid#62834PurposeLim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsT710 hfq leader
gfp
UseSynthetic BiologyTagsm9::glmS leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2027
Plasmid#62835PurposeLim Lab plasmid: AmpR + PLlacO-1::T710 hfq::T1terminator + PLtetO-1::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsT710 hfq leader
gfp
UseSynthetic BiologyTagsglmS leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2053
Plasmid#62836PurposeLim Lab plasmid: AmpR + PLlacO-1::T710::hfq::T1 termintor + PLtetO-1m9::ompC::gfp::T1T2 terminator + ColE1DepositorInsertsT710 hfq leader
gfp
UseSynthetic BiologyTagsm9::ompC leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL2073
Plasmid#62837PurposeLim Lab plasmid: AmpR + PconShortNoHind::glmZ::Asp terminator + PLlacO-1::T710 hfq::T1 terminator + PLtetO-1m9::glmS::gfp::T1T2 terminator + ColE1DepositorInsertsglmZ leader
T710 hfq leader
gfp
UseSynthetic BiologyTagsm9::glmSExpressionBacterialMutationPromoterpConShortNoHind, pLlacO-1, and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only