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Plasmid#16840DepositorInsertpt beta-catenin-myc (ctnnb1.L Frog)
UseXenopus expressionTags6x mycExpressionMutationfour point mutations introduced into the GSK-3 ph…PromoterAvailable sinceJune 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptII[attB/Ins1]
Plasmid#74100Purpose"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)DepositorInsertsPhi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)
Plasmid#55199PurposeCFP reporter along with FF4 miRNA binding sites and 28 nt sequencesDepositorInsertECFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGL2-200bp
Plasmid#16293DepositorInsertp53 promoter fragment (TP53 Human)
UseLuciferaseTagsExpressionMammalianMutationfragment -188 to +12 relative to first transcript…PromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
His-AgdNK
Plasmid#203226PurposeFor protein expression. A. gambiae deoxynucleoside kinase. Phosphorylates a wide variety of nucleosides at the 5-hydroxyl position. Pet28a plasmid, KAN resistant, N-terminal TEV-cleavable 6xHis tag.DepositorInsertdeoxynucleoside kinase (AgaP_AGAP001585 Anopheles gambiae)
UseTagsN-terminal TEV cleavable 6xHis tagExpressionBacterialMutationPromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNAIIIB rac1 QL
Plasmid#61633Purposemammalian expression of rac1 constitutively active mutantDepositorInsertRac1 (RAC1 Human)
UseTagsExpressionMammalianMutationQ61L, constitutively activePromoterCMVAvailable sinceJan. 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGL2-100bp
Plasmid#16294DepositorInsertp53 promoter fragment (TP53 Human)
UseLuciferaseTagsExpressionMammalianMutationfragment -88 to +12 relative to first transcripti…PromoterAvailable sinceDec. 14, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pSG5-ERRbeta2
Plasmid#52186PurposeExpresses human ERRbeta2 splice variant in mammalian cellsDepositorInsertEstrogen-related receptor beta (ESRRB Human)
UseTagsExpressionMammalianMutationwild type sequence; codon optimized to exclude Ec…PromoterSV40Available sinceApril 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag NtDRMcd (no NLS) SUP
Plasmid#115490PurposeCRISPR-Cas9 SunTag system to target NtDRMcd (without an NLS) to the SUPERMAN locus with two guide RNAsDepositorInsertg2_U6_g1_U6_NOS_NLS_GB1_noNLS_linker_DRMcd_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceMarch 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
PXPR007 sgGFP-2
Plasmid#74961PurposeCas9 + sgGFP-2 with blasticidin selectionDepositorInsertGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJZC519
Plasmid#62278PurposeYeast dCas9-VP64 expression plasmidDepositorInsertdCas9-VP64
UseTagsHas a VP64 fusionExpressionYeastMutationHas mutations in the RuvC1 and HNH domains to ren…PromoterpTdh3Available sinceMay 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEG302 22aa SunTag VP64 gRNA4 (FWA)
Plasmid#120249PurposeCRISPR-Cas9 SunTag system to target VP64 to the FWA locus with a single guide RNADepositorInsertg4_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_3xNLS_linker_10xGCN4_OCS
UseCRISPR and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFAB822
Plasmid#47856PurposeBIOFAB reporter plasmid for measuring BBa_B1006 termination efficiencyDepositorInsertBBa_B1006 terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO11Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB803
Plasmid#47847PurposeBIOFAB reporter plasmid for measuring T7 early termination efficiencyDepositorInsertT7 early terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO2Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK2
Plasmid#118092PurposeExpresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorInsertHomo sapiens Sphingosine Kinase 2 (SPHK2 Human)
UseTagsFLAGExpressionYeastMutationPromoterGAL1, 10Available sinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSV human p55 alpha (HA tag)
Plasmid#11498DepositorInsertp55 alpha (PIK3R1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_hH1
Plasmid#102847PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_hH1
UsePcr template for stagr insertsTagsExpressionMutationPromoterU6Available sinceAug. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_hU6
Plasmid#102843PurposeCan be used as PCR template for a STAgR reactionDepositorInsertgRNAScaffold_hU6promoter
UseAs pcr templateTagsExpressionMutationPromoterAvailable sinceJan. 25, 2019AvailabilityAcademic Institutions and Nonprofits only