pSG5-ERRbeta2
(Plasmid
#52186)
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PurposeExpresses human ERRbeta2 splice variant in mammalian cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52186 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepSG5
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Backbone manufacturerAgilent
- Backbone size w/o insert (bp) 4100
- Total vector size (bp) 5615
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEstrogen-related receptor beta
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SpeciesH. sapiens (human)
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Insert Size (bp)1515
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Mutationwild type sequence; codon optimized to exclude EcoRI, BamHI, and SbfI sites; made shRNA resistant
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Entrez GeneESRRB (a.k.a. DFNB35, ERR beta-2, ERR2, ERRb, ERRbeta2, ESRL2, NR3B2)
- Promoter SV40
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site BamHI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bySynthesized by Genewiz
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSG5-ERRbeta2 was a gift from Rebecca Riggins (Addgene plasmid # 52186 ; http://n2t.net/addgene:52186 ; RRID:Addgene_52186) -
For your References section:
ERRbeta Splice Variants Differentially Regulate Cell Cycle Progression. Heckler MM, Riggins RB. Cell Cycle. 2014 Dec 12:0. 10.4161/15384101.2014.972886 PubMed 25496115