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Plasmid#118868Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal tagRFPDepositorInsertNcoI-HIS6-3C-tagRFP-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease, tagRFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC171
Plasmid#118888Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal SNAP tagDepositorInsertNcoI-HIS6-MBP-3C-SNAP-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease; SNAP tagExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC202
Plasmid#118880Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mKate2, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mKate2-3C-HIS6-stop-HindIII cassette
UseTagsmKate2, HIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOCC139
Plasmid#118872Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and C-terminal monomeric GFPDepositorInsertNcoI-HIS6-3C--NotI-ccdB-AscI-mGFP-stop-HindIII cassette
UseTagsHIS6, cleavable with 3C protease and mGFPExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0077
Plasmid#117644PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_Protein kinase superfamily protein {AT1G73460}(pEPOR1CB0099)and sgRNA_Protein kinase superfamily protein {AT1G73460}(pEPOR1CB0110)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR2KN0075
Plasmid#117642PurposeLevel2 MoClo construct, containing level1 plant expression cassettes for SaCas9(pEPOR1CB0015) and sgRNA_Flavin-binding monooxygenase family protein {AT1G62600}(pEPOR1CB0098)and sgRNA_Flavin-binding monooxygenase family protein {AT1G62590}(pEPOR1CB0109)DepositorInsert[35S:SaCas9(pEPOR1CB0015) ] +[AtU6-26:sgRNA]
UseTagsExpressionPlantMutationPromoterAvailable sinceNov. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0109
Plasmid#117571PurposeLevel1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Flavin-binding monooxygenase family protein {AT1G62590} in A. thalianaDepositorInsertPromoter_AtU6-26 + sa_gRNA_Flavin-binding monooxygenase family protein {AT1G62590} A. thaliana
UseTagsExpressionPlantMutationPromoterAtU6-26 (pICSL90002)Available sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0098
Plasmid#117570PurposeLevel1 Golden Gate Cassette: sgRNA cassette for spCas9, targeting Flavin-binding monooxygenase family protein {AT1G62600} in A. thalianaDepositorInsertPromoter_AtU6-26 + sa_gRNA_Flavin-binding monooxygenase family protein {AT1G62600} A. thaliana
UseTagsExpressionPlantMutationPromoterAtU6-26 (pICSL90002)Available sinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0014
Plasmid#117550PurposeLevel 1 Golden Gate Cassette: AsCas12a expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + AsCas12a (with stop codon)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0011
Plasmid#117547PurposeLevel 1 Golden Gate Cassette: eCas9 1.1 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + Sp-eCas9 1.1 (no stop codon) (pEPOR0SP0019 + YFP (pICSL50005) + Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0010
Plasmid#117546PurposeLevel 1 Golden Gate Cassette: eCas9 1.0 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + Sp-eCas9 1.0 (no stop codon) (pEPOR0SP0017) + YFP (pICSL50005) + Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0009
Plasmid#117545PurposeLevel 1 Golden Gate Cassette: Cas9-K855A expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-KA (no stop codon)(pEPOR0SP0015) + YFP (pICSL50005)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0008
Plasmid#117544PurposeLevel 1 Golden Gate Cassette: Cas9-DE expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-DE (no stop codon)(pEPOR0SP0001) + YFP (pICSL50005)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0002
Plasmid#117543PurposeLevel 1 Golden Gate Cassette: Cas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + SpCas9-p (no stop codon)(pEPOR0SP0009) + YFP (pICSL50005)+ Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSKB3-SaGGR-F219L (JBEI-7186)
Plasmid#110151PurposepSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-F219L mutant geneDepositorInsertGGR
UseTagsTEV-cleavable His-tagExpressionBacterialMutationSaGGR-F219L mutant genePromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati MCS Exon
Plasmid#110119PurposeExpression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicingDepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMT dati Exons 1-3
Plasmid#110113PurposeDrosophila dati minigene plasmid that expresses both a linear RNA and a circular RNADepositorInsertdati (dati Fly)
UseTagsExpressionInsectMutationPromoterMetallothionein Promoter (pMT)Available sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD2024
Plasmid#101368PurposeName: pCBG68-RBPjkΔA. RBPjk binding site A mutation in pCBG68-LANApi (pDD2002).DepositorInsertRBPjk binding site A mutation in pCBG68-LANApi (pDD2002).
UseLuciferaseTagsExpressionMutationRBPjk binding site A mutation in pCBG68-LANApi (p…PromoterAvailable sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2015
Plasmid#101365PurposeName: pCBR-K14ΔTATA. K14 TATA box mutation in pCBR-K14 (pDD2005).DepositorInsertK14 TATA box mutation in pCBR-K14 (pDD2005)
UseLuciferaseTagsExpressionMutationK14 TATA box mutation in pCBR-K14 (pDD2005)PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDD2023
Plasmid#101367PurposeName: pCBR-RBPjkΔB. RBPjk binding site B mutation in pCBR-K14 (pDD2005).DepositorInsertRBPjk binding site B mutation in pCBR-K14 (pDD2005).
UseLuciferaseTagsExpressionMutationRBPjk binding site B mutation in pCBR-K14 (pDD200…PromoterAvailable sinceOct. 31, 2017AvailabilityAcademic Institutions and Nonprofits only