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VGAT scFv [L118/14]

Plasmid

#206754

Purpose

Mammalian Expression of VGAT scFV. Derived from hybridoma L118/14 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

VGAT (Mus musculus) recombinant scFV (Slc32a1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hDlx-GiDREADD-dTomato-Fishell-5

Plasmid

#83896

Purpose

Gi-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

hM4Di

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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HCN4 scFv [N114/10]

Plasmid

#190526

Purpose

Mammalian Expression of HCN4 scFV. Derived from hybridoma N114/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

HCN4 (Homo sapiens) recombinant scFV (HCN4 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hDlx-Flex-GFP-Fishell_6

Plasmid

#83895

Purpose

Cre recombinase-dependent GFP expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, AAV2, AAV8, and AAV9

Insert

eGFP

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

hDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP

Plasmid

#112677

Purpose

An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

Nuclear-localized floxed-mCherry EGFP

Use

AAV

Tags

Nuclear localization signal

Expression

Mutation

Promoter

EF1a

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hDlx-GqDREADD-dTomato-Fishell-4

Plasmid

#83897

Purpose

Gq-DREADD (P2A) nuclear dTomato expression in forebrain GABA-ergic interneurons under the control of the hDlx enhancer element

Depositor

Gordon Fishell

Article

Dimidschstein et al Nat Neurosci. 2016 Oct 31. doi: 10.1038/

Has Service

AAV Retrograde, AAV1, and AAV9

Insert

hM3Dq

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hDlx

Available since

Oct. 31, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-pGK-nAChRb2(E61C)-IRES-GFP

Plasmid

#164777

Purpose

cysteine mutated nAChR beta2 subunit (E61C) for the attachment of a photoswitchable tethered ligand

Depositor

Alexandre Mourot

Article

Durand-de Cuttoli et al Elife. 2018 Sep 4;7. pii: 37487. doi: 10

Insert

nAChR b2 E61C - IRES- GFP (Chrnb2 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

E61C

Promoter

pGK

Available since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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pOTTC763 - pX458 with rat Rosa26 gRNA A

Plasmid

#113161

Purpose

A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Insert

gRNA for rat Rosa26

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-ProA1-GCaMP6s

Plasmid

#126002

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GCaMP6s

Use

AAV

Tags

Expression

Mutation

Promoter

ProA1

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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VI_pAAV-ProA7-GFP-WPRE

Plasmid

#125891

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA7

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pCDH-EF1a-hDNAJC6-T2A-copGFP

Plasmid

#170443

Purpose

Lentiviral vector expressing human DNAJC6 with copGFP reporter gene, under control of EF1alpha promoter

Depositor

Sang-Hun Lee

Article

Wulansari et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabb

Insert

human DNAJC6 (DNAJC6 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

June 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-Halo-Tag-128-425-Akap9

Plasmid

#196873

Purpose

Expression of fragment 128-425 from A-Kinase Anchoring Protein 9 (Akap9) fused to Halo-Tag. Used to displace endogenous Akap9 from Golgi

Depositor

Frank Bradke

Article

Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

Insert

Halo-Tag-Akap9 (128-425) (Akap9 Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Quimeric CAG

Available since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO {mCAR}off-{ChETA-mRuby2}on-W3SL

Plasmid

#111390

Purpose

AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection), Cre-ON ChETA-mRuby2 (for optogenetic activation), and W3SL regulatory cassette (for maximize cloning capacity)

Depositor

Adam Kepecs

Article

Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

Inserts

mCAR (Cxadr Mouse)
ChETA-mRuby2

Use

AAV

Tags

Myc and mRuby2

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV2-ProC3-Cre/mCherry

Plasmid

#126006

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

Cre-2A-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

ProC3

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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123_pAAV-ProC1-CatCh-GFP-WPRE

Plasmid

#125937

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProC1

Available since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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LRP4 (extracellular) scFv [N207/27]

Plasmid

#190537

Purpose

Mammalian Expression of LRP4 (extracellular) scFV. Derived from hybridoma N207/27.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

LRP4 (extracellular) (Mus musculus) recombinant scFV (Lrp4 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
206_pAAV-ProD17-CatCh-GFP-WPRE

Plasmid

#125993

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProD17

Available since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
136_pAAV-ProA1-CatCh-GFP-WPRE

Plasmid

#125886

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA1

Available since

Sept. 16, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Gephyrin scFv [L106/23]

Plasmid

#206775

Purpose

Mammalian Expression of Gephyrin scFV. Derived from hybridoma L106/23 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Gephyrin (Homo sapiens) recombinant scFV (GPHN Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
57_pAAV-ProA14-CatCh-GFP-WPRE

Plasmid

#125897

Purpose

Targeting expression to neuronal and glial cell types in mice, non-human primates, and human retina

Depositor

Botond Roska

Article

Juttner et al Nat Neurosci. 2019 Jul 8. pii: 10.1038/s

Insert

CatCh-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

ProA14

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge