Addgene

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pZA53

Plasmid

#174766

Purpose

Entry clone for Golden Gate assembly

Depositor

Sophien Kamoun

Article

Harant et al Plant Physiol. 2022 Jan 20;188(1):70-80.

Insert

GG_NbZAR1syn without STOP codon

Use

Unspecified

Tags

Expression

Mutation

WT

Promoter

Available since

Oct. 5, 2021

Availability

Academic Institutions and Nonprofits only

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cisRAJ31-GFP-LAA

Plasmid

#69928

Purpose

Expresses cisRAJ31-GFP-LAA, a cis-repressed mRNA regulated by RAJ31.

Depositor

Alfonso Jaramillo

Article

Rostain et al Biodes Res. 2020 Sep 12;2020:1916789. do

Insert

PllacO1-cisRAJ31-GFP-LAA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

PlLacO1

Available since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only

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C413-E31: attB4-TRE3Gp>-attB1r

Plasmid

#162924

Purpose

Gateway attB4/attB1r entry clone for inducible mammalian expression using the TRE3G promoter

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

Tet-regulated TRE3G minimal promoter

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 20, 2021

Availability

Academic Institutions and Nonprofits only

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pMM0617

Plasmid

#128993

Purpose

loxP-KIURA3-loxP preassembled entry vector

Depositor

Megan McClean

Article

An-Adirekkun et al Biotechnol Bioeng. 2019 Dec 2. doi: 10.1

Insert

KanR-ColE1 URA 5' homology-ConLS'-sfGFP dropout-ConRE'-loxP-KIURA3-loxP-URA3 5' homology

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ159

Plasmid

#69308

Purpose

Gateway entry vector with hSpCas9D10A

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9D10A (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Expression

Mutation

D10A mutation; NLS fusion

Promoter

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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Wnt7A STOP

Plasmid

#35877

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt7A (WNT7A Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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p3E-mCherry-HA no-pA

Plasmid

#82592

Purpose

3' entry vector with HA epitope fused to C-terminus of mCherry without pA for imaging, epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

mCherry-HA (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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776 pME-miR-126

Plasmid

#26033

Depositor

Nathan Lawson

Article

Nicoli et al Nature. 2010 Apr 22. 464(7292):1196-200.

Insert

miR-126 (mir126a Zebrafish)

Use

Entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 26, 2010

Availability

Academic Institutions and Nonprofits only
pPTK007-3a-αMFΔ

Plasmid

#84966

Purpose

Type 3a, α-mating factor pre-sequence shortened - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor, pre-sequence shortened secretion tag

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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p3E-HA no-pA

Plasmid

#82593

Purpose

3' entry vector containing HA epitope fused to C-terminus of mCherry without pA for epitope labeling/purification

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

HA tag (no pA)

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pPTK008-3a-αMFΔ no Kex

Plasmid

#84967

Purpose

Type 3a, α-mating factor no Kex recognition site - secretion signal

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

α-mating factor no Kex recognition site secretion tag

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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p5EsheE1

Plasmid

#90116

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of She

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

sheE1 (she Zebrafish)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA4/HisMaxB-YAP2-1st WW mutant

Plasmid

#18998

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Tryptophan 199 changed to Alanine, and Proline 2…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
pYPQ158

Plasmid

#69307

Purpose

Gateway entry vector with hSpCas9 (Human codon optimized)

Depositor

Yiping Qi

Article

Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

Insert

hSpCas9 (Human codon optimized)

Use

CRISPR

Tags

3XFLAG

Expression

Mutation

NLS fusion

Promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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Wnt10B STOP

Plasmid

#35884

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt10B (WNT10B Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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Wnt5A Non-STOP

Plasmid

#35893

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt5A (WNT5A Human)

Use

Entry vector

Tags

Expression

Mutation

None

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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pSB3C5-proC-B0032-E0051

Plasmid

#107242

Purpose

Expresses lacZa-GFP fusion from constitutive proC promoter

Depositor

Robert Sauer, Joseph Davis

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProC-RBS(B0032)-Gemini (E0051)

Use

Tags

Expression

Mutation

Promoter

proC

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pSB3C5-proB-B0032-E0051

Plasmid

#107243

Purpose

Expresses lacZa-GFP fusion from constitutive proB promoter

Depositor

Robert Sauer, Joseph Davis

Article

Davis et al Nucleic Acids Res. 2011 Feb;39(3):1131-4

Insert

ProB-RBS(B0032)-Gemini (E0051)

Use

Tags

Expression

Mutation

Promoter

proB

Available since

Aug. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4/HisMaxB-YAP2-2nd WW mutant

Plasmid

#18999

Depositor

Marius Sudol

Article

Oka et al J Biol Chem. 2008 Jul 17. ():.

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

Tryptophan 258 changed to Alanine, and Proline 2…

Promoter

Available since

Sept. 8, 2008

Availability

Academic Institutions and Nonprofits only
Wnt2 STOP

Plasmid

#35869

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2 (WNT2 Human)

Use

Entry vector

Tags

Expression

Mutation

Silent Mutation G (816) --> A

Promoter

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

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