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Showing: 5021 - 5040 of 5230 results

  1. Recruited polymerase expression - pCMV-T7-Phi29(+exo)-CC(N5) (LM2761)

    Plasmid
    #208970
    Purpose
    Recruited Phi29(+exo) DNA polymerase with a C-terminal N5 coiled coil (CC) domain, expressed from CMV or T7 promoters.
    Depositor
    Benjamin Kleinstiver
    Article
    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103
    Insert
    Phi29(+exo)-BPNLS-CC(N5)
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and T7
    Available since
    Nov. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    208970-plasmid-map-sequence-id-415821

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  8. TKIT NFL gRNA1 gRNA2

    Plasmid
    #182681
    Purpose
    Expression of spCas9, gRNA targeting intron 3 and gRNA targeting 3'UTR of the mouse Nefl gene. Can be used for the tagging of endogenous NFL via Targeted Knock-In with Two guides (TKIT) approach.
    Depositor
    Ivana Nikić-Spiegel
    Article
    Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:
    Insert
    two Nefl-targeting gRNAs, one targets intron 3 and the other 3'UTR of the Nefl gene (Nefl Mouse)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 and chicken beta-actin promoter
    Available since
    May 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182681-plasmid-map-sequence-id-363020

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  11. PEA1-Nuclease

    Plasmid
    #176528
    Purpose
    Delivers all prime editing nuclease components in a single plasmid
    Depositor
    Paul Thomas
    Article
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Insert
    CbH-Cas9-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    GC to CA point mutation in SpCas9 to restore nucl…
    Promoter
    CbH for Cas9, hU6 for gRNAs
    Available since
    Dec. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    176528-plasmid-map-sequence-id-350557

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  12. pDONR207-MUM2sp-Citrine-ADPG2

    Plasmid
    #170725
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the Citrine and the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)
    Depositor
    George Haughn
    Article
    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629
    Insert
    ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
    Use
    Gateway donor vector / entry clone
    Tags
    Citrine tag (714bp)
    Expression
    Mutation
    Promoter
    Available since
    Nov. 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    170725-plasmid-map-sequence-id-345738

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  13. pDONR207-MUM2sp-ADPG2

    Plasmid
    #170723
    Purpose
    Gateway (Invitrogen) entry clone (pDONR207) containing the MUM2 (At5g63800) signal peptide (84bp) fused in-frame to the coding sequence of the HG degrading enzyme ADPG2 (At2g41850.1)
    Depositor
    George Haughn
    Article
    McGee et al Plant Cell Physiol. 2021 Jun 1. pii: 629
    Insert
    ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE 2 (PGAZAT Mustard Weed)
    Use
    Gateway donor vector / entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 11, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    170723-plasmid-map-sequence-id-345619

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  14. pEGB_3alpha2 OplacI:mini35S:RDF:Tnos (GB1534)

    Plasmid
    #160617
    Purpose
    TU for the regulated expression of the PhiC31 phage recombination directionality factor (RDF) gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    RDF
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    mini35s
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160617-plasmid-map-sequence-id-322019

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  16. pEGB3 alpha2 OplexA:mini35S:PhiC31int:Tnos (GB1529)

    Plasmid
    #160614
    Purpose
    TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LexA operator fused to the minimal 35S promoter.
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    PhiC31
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    mini35s
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160614-plasmid-map-sequence-id-322024

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  17. pEGB_3alpha1 OplacI:mini35S:phiC31:Tnos (GB1530)

    Plasmid
    #160615
    Purpose
    TU for the regulated expression of the PhiC31 phage integrase gene driven by a synthetic promoter consisting of the LacI operator fused to the minimal 35S promoter.
    Depositor
    Diego Orzaez
    Article
    Bernabe-Orts et al Nucleic Acids Res. 2020 Apr 6;48(6):3379
    Insert
    PhiC31
    Use
    Synthetic Biology
    Tags
    Expression
    Plant
    Mutation
    BsaI and BsmBI sites removed
    Promoter
    mini35s
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    160615-plasmid-map-sequence-id-322022

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  18. BE3-YE1

    Plasmid
    #132943
    Purpose
    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.
    Depositor
    Hui Yang
    Article
    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:
    Insert
    BE3-(W90Y-R126E)
    Use
    CRISPR; Base editor
    Tags
    Expression
    Mammalian
    Mutation
    BE3-(W90Y-R126E)
    Promoter
    Available since
    Nov. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    132943-plasmid-map-sequence-id-314816

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Showing: 5021 - 5040 of 5230 results