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Plasmid#111902PurposessAAV genome with Ple346 (NEFM MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle346 (NEFM Human)
UseAAVTagsExpressionMutationPromoterPle346 (NEFM MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEMS2242
Plasmid#111897PurposessAAV genome with Ple321 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle321 (TUBB3 Human)
UseAAVTagsExpressionMutationPromoterPle321 (TUBB3 MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
mDlx-BiPOLES-mCerulean
Plasmid#154946PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterminimal DlxAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
hSyn-DIO-BiPOLES-mCerulean
Plasmid#154950PurposeOptogenetic tool for blue-light inhibition and red-light excitation of neuronsDepositorInsertBiPOLES
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsinAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
px551-mSncg-0.27kb-HA-SpCas9
Plasmid#153208PurposeTruncated Mouse gamma-synuclein (mSncg-0.27kb) promoter-mediates HA and Cas9 expression in retinal ganglion cellsDepositorInsertCas9
UseAAV and CRISPRTagsHAExpressionMammalianMutationPromoterTruncated mSncg (0.27kb)Available sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV302
Plasmid#119943PurposeExpresses N-terminal Npu DnaE Intein-SaKKH-BE3 in mammalian cellsDepositorInsertN-terminal SaKKH-BE3(739) - N-Intein (Npu DnaE)
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS2274
Plasmid#111900PurposessAAV genome with Ple344 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle344 (TUBB3 Human)
UseAAVTagsExpressionMutationPromoterPle344 (TUBB3 MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV312.3
Plasmid#119944PurposeExpresses C-terminal Npu DnaE Intein-SaKKH-BE3, tagRFP, and sgRNA in mammalian cellsDepositorInsertC-Intein (Npu DnaE) - C-terminal (740)SaKKH-BE3
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMS2273
Plasmid#111899PurposessAAV genome with Ple343 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle343 (TUBB3 Human)
UseAAVTagsExpressionMutationPromoterPle343 (TUBB3 MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
mOrange-P2A-hKvb1
Plasmid#154084PurposeIndependent expression of mOrange and human Kvbeta 1 under human synapsin 1 promoterDepositorInsertmOrange-P2A-human Kvb1 (KCNAB1 Synthetic, Human)
UseAAVTagsExpressionMammalianMutationPromoterhuman synapsin 1Available sinceNov. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
DNMT3ACD-CRY2-EGFP
Plasmid#82556PurposeEncodes DMNT3 under LITE2.0 systemDepositorUseAAVTags2A, NLS(alpha-imp), and NLS-VP64ExpressionMammalianMutationCRY2PHR (N-terminal photolyase homology region do…PromoterEF-1alphaAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEMS2272
Plasmid#111898PurposessAAV genome with Ple342 (TUBB3 MiniPromoter) driving an emerald GFP (EmGFP) reporter. Contains WPREDepositorInsertPle342 (TUBB3 Human)
UseAAVTagsExpressionMutationPromoterPle342 (TUBB3 MiniPromoter)Available sinceAug. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEJS1553 pLenti-U1a-SpCas9-miniU6-tracrRNA
Plasmid#211819Purposelentiviral vector expressing SpCas9 and tracrRNA for stable cell line extablishmentDepositorInsertSpCas9, tracrRNA and blasticidin resistance gene
UseLentiviralTagsExpressionMammalianMutationPromoterU1a promoter and miniU6 promoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2194-RP_PiggyBac_SpCas9ABE8e-tracrRNA-Puro
Plasmid#211821PurposePiggyBac vector expressing SpCas9-ABE8e with tracrRNA for stable cell line establishmentDepositorInsertSpCas9-ABE8e, tracrRNA and Puromycin resistance gene
UseTagsExpressionMammalianMutationPromoterchicken β-actin promoter, U6 promoter and hPGK pr…Available sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS-HZ03-pLenti-U1a-mSpCas9-U6-tracrRNA-PuroR
Plasmid#211815Purposelentiviral vector expressing SpCas9 and tracrRNA for stable cell line establishmentDepositorInsertSpCas9, tracrRNA and puromycin selection gene
UseLentiviralTagsExpressionMammalianMutationPromoterU1a promoter, U6 promoter, and hPGK promoterAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Donor-CD55-NeoR
Plasmid#153551PurposeUsed as a donor vector for the targeted knock-in of a CD55 mutation. This plasmid carries a neomycin resistance gene cassetteDepositorInsertmutant CD55 (a 1415-bp fragment from intron 3, exon 4 and intron 4, with a 1-bp mutation in exon 4), divided into 5' and 3' arms
UseAAV and Cre/Lox; Donor plasmid/targeting vectorTagsN/AExpressionMutationa 1-bp mutation in exon 4PromoterNo promoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
Donor-PIGA-NeoR
Plasmid#153549PurposeUsed as a donor vector for the targeted knock-in of a PIGA mutation. This plasmid carries a neomycin resistance gene cassetteDepositorInsertmutant PIGA (a 1,991-bp fragment from intron 5 and exon 6 with a 3-bp mutation in exon 6), divided into 5' and 3' arms
UseAAV and Cre/Lox; Donor plasmid/targeting vectorTagsN/AExpressionMutationa 3-bp mutation in exon 6PromoterNo promoterAvailable sinceDec. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
sgCRY1
Plasmid#176511PurposeExpresses sgRNA in mammalian cellsDepositorUseAAVTagsmCherry (K14E, N210D, E249G)ExpressionMutationPromoterU6, hSynAvailable sinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
px552-U6-gRNA1-U6-gRNA2-CMV-eGFP
Plasmid#211760PurposePaired gRNAs (sgRNA1 and 2) targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.DepositorInsertgRNA1 and gRNA2 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
px552-U6-gRNA1-CMV-eGFP
Plasmid#211758PurposesgRNA1 targeting Exon 1 of VEGFA gene conserved across mouse, rhesus macaque, and human.DepositorInsertgRNA1 targeting VEGF-A (VEGFA Human, Mouse, M. mulatta (rhesus macaque))
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 1, 2024AvailabilityAcademic Institutions and Nonprofits only