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Plasmid#175547PurposeCo-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cellsDepositorInsertH2B-HaloTag7 and Tomm20-HaloTag9
UseTagsExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Beta4Gal-HaloTag7-T2A-Lyn11-SNAPf
Plasmid#175524PurposeCo-expression of HaloTag7 (gogli) and SNAPf-tag (membrane inner leaflet) in mammalian cellsDepositorInsertBeta4Gal-HaloTag7 and Lyn11-SNAPf
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
UPB-duodenum (UPB6)
Plasmid#180785PurposeUnique Projection Barcode (UPB6)-Duodenum for Projection-seqDepositorInserthM4D(Gi) (partial )
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceAug. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoB-ATP
Plasmid#193819PurposeCMV overexpression of ChemoB-ATP in mammalian cell linesDepositorInsertChemoB-ATP
UseTagsExpressionMammalianMutationEBFP2:N39Y, A206K; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
NLS-tdPCP-GFP
Plasmid#183934PurposePCP tandem dimer binding to PP7 RNA stem loops; labeled with GFPDepositorInserttdPCP-GFP
UseTagsNLSExpressionMammalianMutationnonePromoterCMV (TATA box removed)Available sinceJune 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMA-SpCas9-g4
Plasmid#80787PurposeBsaI-based cloning of SpCas9 gRNA guide sequence, position 4/14/24 in the arrayDepositorInsertCRISPR gRNA expression cassette (for SpCas9)
UseCRISPRTagsnaExpressionMammalianMutationPromoterU6Available sinceSept. 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJFRC201-10XUAS-FRT>STOP>FRT-myr::smGFP-HA
Plasmid#63166PurposeGAL4-responsive UAS "flp-On" spaghetti monster GFP-HA reporterDepositorInsert"spaghetti monster GFP-HA"
UseTagsN-myristoylationExpressionInsectMutationPromoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abc
Plasmid#80617PurposepBIG2abc - Step2 vector abc (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoL-ATP
Plasmid#193831PurposeCMV overexpression of ChemoL-ATP in mammalian cell linesDepositorInsertChemoL-ATP
UseTagsExpressionMammalianMutationEGFP:A206K; HT7: L271EPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abcde
Plasmid#80619PurposepBIG2abcde - Step2 vector abcde (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG2abcd
Plasmid#80618PurposepBIG2abcd - Step2 vector abcd (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1c
Plasmid#80613PurposepBIG1c - Step1 vector c (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1d
Plasmid#80614PurposepBIG1d - Step1 vector d (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBIG1e
Plasmid#80615PurposepBIG1e - Step1 vector e (biGBac system for expression of protein complexes in insect cells)DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11274:pHR-hU6-crTet-HSP-hyperdCas12a-miniVPR-CMV-mCherry
Plasmid#210603Purposeheat shock promoter - activated hyperdCas12a-based transcriptional activatorDepositorInserthyperdCas12a-miniVPR
UseCRISPR and LentiviralTagsExpressionMutationPromotertruncated hsp70Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
P1-EYFP-pA (Construct 5)
Plasmid#55197PurposeSynthetic promoter responsive to gRNA1DepositorInsertEYFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterMinimal Ade MLPAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT-ChemoR-ATP
Plasmid#193820PurposeCMV overexpression of ChemR-ATP in mammalian cell linesDepositorInsertChemoR-ATP
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lifeact-HaloTag11-T2A-Tomm20-HaloTag7
Plasmid#175526PurposeCo-expression of HaloTag11 (actin) and 7 (mitochondrial surface) in mammalian cellsDepositorInsertLifeact-HaloTag11 and Tomm20-HaloTag7
UseTagsExpressionMammalianMutationHaloTag11 = HaloTag7-M175WPromoterAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
8xNFAT-ZsG-mCD8
Plasmid#162747PurposeNFAT-driven ZsGreen-1 reporter gene with mouse CD8 geneDepositorInsert8x of NFAT binding motif followed by ZsGreen-1 fluorescent protein gene
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYL-kasOp-FnCas12a3
Plasmid#177875PurposeExpresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.DepositorInsertStreptomyces codon optimized the FnCas12a mutant EP16
UseCRISPRTagsExpressionMutationPromoterkasOp*Available sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only