Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Showing: 5001 - 5020 of 13935 results

  1. pCL1314

    Plasmid
    #217956
    Purpose
    Expression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3.1 (H3C1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    GGG insertion between A95 and C96
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    217956-plasmid-map-sequence-id-430210

    Enlarge

  2. pCL1315

    Plasmid
    #217957
    Purpose
    Expression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.
    Depositor
    Andrew Bowman
    Article
    Pardal et al Elife. 2022 Sep 6;11:e81755. doi: 10.755
    Insert
    Histone H3.1 (H3C1 Human)
    Use
    Tags
    eGFP
    Expression
    Mammalian
    Mutation
    F84A, L92A, Y99A
    Promoter
    CMV
    Availability
    Academic Institutions and Nonprofits only
    217957-plasmid-map-sequence-id-430211

    Enlarge

  5. pJQR3

    Plasmid
    #218289
    Purpose
    Introduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    HO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036W
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    218289-plasmid-map-sequence-id-433691

    Enlarge

  6. pToxEXPr6

    Plasmid
    #218290
    Purpose
    Introduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3
    Depositor
    Claudia Vickers
    Article
    McDonnell et al bioRxiv 2024.03.26.586389
    Insert
    hph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    218290-plasmid-map-sequence-id-433735

    Enlarge

  13. pHLmMBP-8

    Plasmid
    #72347
    Purpose
    Mammalian expression of cytoplasmic mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Availability
    Academic Institutions and Nonprofits only
    72347-plasmid-map-sequence-id-133755

    Enlarge

  14. pHLmMBP-11

    Plasmid
    #72349
    Purpose
    Mammalian expression of secreted mMBP TEV cleavage site-linked fusion proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, HA-tag, Strep-tag II, and mMBP with C-t…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Availability
    Academic Institutions and Nonprofits only
    72349-plasmid-map-sequence-id-133760

    Enlarge

  15. pBS/U6/RNAi gamma-tubulin shRNA mouse

    Plasmid
    #70666
    Purpose
    Reduced the expression of gamma-tubulin in murine cells
    Depositor
    Maria Alvarado-Kristensson
    Article
    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1
    Insert
    5´-GGCTCATGATGGCCAACCACCAAGCTTGGTGGTTGGCCATCATGAGCCCTTTTTC-3´ 5´-CCGAGTACTACCGGTTGGTGGTTCGAACCACCAACCGGTAGTACTCGGGAAAAAGTTAA-3´ (Tubg1, Tubg2 Mouse)
    Use
    U6 promoter
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Availability
    Academic Institutions and Nonprofits only
    70666-plasmid-map-sequence-id-133021

    Enlarge

Showing: 5001 - 5020 of 13935 results