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Plasmid#115833PurposeEncodes codon optimized 3xFLAG-SIVCPZ-TAN3 Vpr (DQ374658) and puromycin resistance proteinDepositorInsertSIVCPZ-TAN3 Vpr (DQ374658)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-CPZ-LB7 vpr
Plasmid#115834PurposeEncodes codon optimized 3xFLAG-SIVCPZ-LB7 Vpr (DQ373064) and puromycin resistance proteinDepositorInsertSIVCPZ-LB7 Vpr (DQ373064)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS-SIV-GOR -vpr
Plasmid#115835PurposeEncodes codon optimized 3xFLAG-SIVGOR Vpr (FJ424871) and puromycin resistance proteinDepositorInsertSIVGOR Vpr (FJ424871)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPS- HIV2-ROD vpx
Plasmid#115816PurposeEncodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance proteinDepositorInsertHIV2ROD Vpx (M15390)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
BPK1520_puroR
Plasmid#173901PurposeSpCas9 guide cloning vector derived from BPK1520 that allows for puromycin-based selectionDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCMV/U6Available sinceNov. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICE-FLAG-NAT10-siR-G641E
Plasmid#59366PurposePlasmid for constitutive or doxycycline-inducible expression of G641E mutant of human NAT10 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertNAT10 NM_024662 (NAT10 Human)
UseTagsFLAGExpressionMammalianMutationG641E and silent mutations to render the cDNA res…PromoterCMV-tetAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLJM60 FLAG-RAB35 S22N
Plasmid#107106PurposeLentiviral vector that codes for FLAG-RAB35 S22N (human) protein. Puromycin resistance for mammalian selection and ampicillin resistance for growth in Stbl3 cells.DepositorInsertRAB35 (RAB35 Human)
UseLentiviralTagsFLAG tagExpressionMammalianMutationPromoterCMVAvailable sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRRL-gRNA(anti-cGAS)-Cas9-Puro
Plasmid#130908PurposegRNA targeting human cGAS; Cas9 insert; Puromycin selection markerDepositorInsertCas9
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-Pur-dCas9-KRAB-MeCP2_hU6-A10
Plasmid#196079PurposeConstitutive CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.DepositorInsertgRNA targeting Adam10
UseCRISPRTagsExpressionMammalianMutationCloned using SapI sites - destroyed after inserti…PromoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPyPGK-EGFP-TM-IRES-Pac
Plasmid#183604PurposeMammalian expression vector for membrane-tethered EGFP from the mouse Pgk1 promoter. Confers puromycin resistance.DepositorInsertEGFP-TM
UseTagsHuman PDGFRB transmembrane domain and Mouse IgGK …ExpressionMammalianMutationPromoterMouse Pgk1Available sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFH2.155_LV-Puro-T2A-mTagBFP2
Plasmid#205553PurposeExpress puromycin resistance marker and mTagBFP2DepositorInsertPuro-T2A-mTagBFP2
UseLentiviralTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pL-SFFV.Reporter.RFP657.PAC
Plasmid#61395PurposeLentiviral vector for RFP657 NHEJ reporter system expression. Allows insertion of target sites between the SFFV promoter and RFP657 for detection of fluorescence knockdown. Puromycin resistance.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterSFFVAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P26
Plasmid#120037PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mAID-CloverDepositorInsertmAID-Clover
UsePcr templateTagsmAID-CloverExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-CT3G-ERP2-MG-IRES2-mNeonGreen
Plasmid#175506PurposeAll-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements upstream of IRES2-mNeonGreen (hEF1a-driven rtTA and puromycin resistance)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE3G-mCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-cGASΔN-HA
Plasmid#130912PurposeExpresses human cGASΔN (aa160-522)-HA; Puromycin selection markerDepositorInsertcGASΔN
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P29
Plasmid#120040PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with Strep-IIDepositorInsertStrep-II
UsePcr templateTagsStrep-IIExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMaCTag-P11
Plasmid#120022PurposeTemplate for C-terminal PCR tagging of mammalian genes (Puromycin selection) with mScarlet-iDepositorInsertmScarlet-i
UsePcr templateTagsmScarlet-iExpressionMutationPromoterNoneAvailable sinceJan. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPyCAG-HA-EGFP-Myc-TM-IRES-Pac
Plasmid#183605PurposeMammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.DepositorInsertEGFP-TM
UseTagsHA, Human PDGFRB transmembrane domain, Mouse IgGK…ExpressionMammalianMutationPromoterCAGAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pscALPS SIV-MND2 vpx
Plasmid#115811PurposeEncodes codon optimized 3xFLAG-SIVMND2 Vpx (AY159322) and puromycin resistance proteinDepositorInsertSIVMND2 Vpx (AY159322)
UseLentiviralTags3x-FLAGExpressionMutationPromoterSFFVAvailable sinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMY-mCherry-P2A-PuroR-HA
Plasmid#163342PurposeRetroviral vector to express cytosolic mCherry and an HA-tagged puromycin selection markerDepositorInsertmCherry
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only