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Plasmid#69579PurposeExpresses p53 tagged with mCeruleanDepositorInsertp53 (TP53 Human)
UseLentiviralTagsmCeruleanExpressionMammalianMutation7 silent mutations in p53 DNA to prevent targetin…PromoterEF1alphaAvailable sinceOct. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOpen-Endonuclease VIII E. coli
Plasmid#165561PurposeBifunctional DNA glycosylase with DNA N-glycosylase and AP lyase activities; The N-glycosylase activity releases damaged pyrimidines, including thymine glycol and uracil glycol. The AP lyase activity cleaves DNA phosphodiester backbone at AP sites via beta and delta-elimination, creating a 1 nucleotide DNA gap with 5' and 3' phosphate termini.DepositorInsertEndonuclease VIII E. coli
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 16, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDpy-7::oxCerulean-oxVenus::Lgg-1
Plasmid#68042PurposeLgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in hypodermis, for monitoring autophagyDepositorInsertpDpy-7::oxCerulean-oxVenus::Lgg-1
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
LoxP3-HA-H2B-Cerulean-2A (SO104)
Plasmid#99618PurposeTo clone gene of interest downstream of LoxP3-HA-H2B-Cerulean-2ADepositorInsertHA-H2B-Cerulean
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVha-6::oxCerulean-oxVenus::Lgg-1
Plasmid#68041PurposeLgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in intestines, for monitoring autophagyDepositorInsertpVha-6::oxCerulean-oxVenus::Lgg-1
UseTagsExpressionWormMutationPromoterAvailable sinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-yECerulean-CaUra3
Plasmid#44648DepositorInsertyECerulean
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 9, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMyo-2::oxCerulean-oxVenus::Lgg-1
Plasmid#68043PurposeLgg-1 tagged with oxCerulean-oxVenus (dFP, or double fluorescent protein), expressed in the pharynx, for monitoring autophagyDepositorInsertpMyo-2::oxCerulean-oxVenus::Lgg-1
UseTagsExpressionWormMutationPromoterAvailable sinceAug. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-Avi-Cerulean-Rpl10a
Plasmid#79880PurposeBeta-actin2 promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)
UseTagsAviExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMTB2-RanGAP-Cerulean-Avi
Plasmid#80068PurposeBeta-actin2 promoter driving C-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequencesDepositorInsertCerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
UseZebrafish biotaggingTagsAviExpressionBacterialMutationPromoterBeta-actin2Available sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
Cerulean(1-158) in pcDNAI/Amp
Plasmid#60897PurposeThis vector attaches Cerulean(1-158) to the N-terminus of a protein.DepositorInsertCerulean(1-158)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
FRT3-HA-H2B-Cerulean-2A (IG158))
Plasmid#99624PurposeTo clone gene of interest downstream of FRT3-HA-H2B-Cerulean-2A cassetteDepositorInsertHA-H2B-Cerulean
UseTagsT2AExpressionMammalianMutationPromoterAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
mTALEAD 16S rRNA-1139T8C
Plasmid#210837PurposemTALEAD expression vectorDepositorInsertFor specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
mTALEAD 16S rRNA-1131T10C
Plasmid#210836PurposemTALEAD expression vectorDepositorInsertFor specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
mTALEAD 16S rRNA-1131T8C
Plasmid#210835PurposemTALEAD expression vectorDepositorInsertFor specific A-to-G base editing to introduce targeted A-to-G mutation in 16S rRNA in chloroplast
UseTagsExpressionPlantMutationPromoterAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
p21(dPCNA)-mCerulean-NeoR
Plasmid#215082PurposeExpressesp21(dPCNA)-mCerulean in mammalian cells.DepositorInsertCDKN1A (CDKN1A Human)
UseTagsmCeruleanExpressionMammalianMutationp21(dPCNA) denotes mutations M147A, D149A, F150A.PromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p21(RxL)-mCerulean-NeoR
Plasmid#215081PurposeExpresses p21(RxL)-mCerulean in mammalian cells.DepositorInsertCDKN1A (CDKN1A Human)
UseTagsmCeruleanExpressionMammalianMutationp21(RxL) denotes mutations: R19A, R20A, L21A, F22APromoterCMVAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
p21(RxL)-mCerulean-PuroR
Plasmid#215078PurposeExpresses p21(RxL)-mCerulean in mammalian cells from a retroviral vector.DepositorInsertCDKN1A (CDKN1A Human)
UseRetroviralTagsmCeruleanExpressionMammalianMutationp21(RxL) denotes mutations: R19A, R20A, L21A, F22APromoterLTRAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p21(dPCNA)-mCerulean-PuroR
Plasmid#215079PurposeExpresses p21(dPCNA)-mCerulean in mammalian cells from a retroviral vector.DepositorInsertCDKN1A (CDKN1A Human)
UseRetroviralTagsmCeruleanExpressionMammalianMutationp21(dPCNA) denotes mutations M147A, D149A, F150A.PromoterLTRAvailable sinceApril 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
MsACR1-mCerulean3-pAAV2-hSyn
Plasmid#204965PurposePanneuronal expression of MsACR1 fused to mCerulean3DepositorInsertMsACR1
UseAAVTagsmCerulean3ExpressionMutationPromoterhSynAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
mCerulean3-BclXL-ActA-s2193
Plasmid#177411PurposeTo express mCerulean3 fused to the N-terminus of the Bcl-2 family chimera protein, Bcl-XL-ActA: Bcl-XL with its membrane binding region swapped for ActA (mitochondrial targeting sequence).DepositorInsertBcl-XL-ActA, BclX-acta
UseTagsmCerulean3ExpressionMammalianMutationPromoterHuman ferritinAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only