Addgene

We narrowed to 1,269 results for: MEO;

Showing: 961 - 1000 of 1269 results

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pEGFP-NDUFV2-mVenus

Plasmid

#185653

Purpose

NDUFV2 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFV2 (NDUFV2 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-Nix-delta TM

Plasmid

#100755

Purpose

Mammalian expression of Nix dominant negative

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Nix-_TM (BNIP3L Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

TM deletion

Promoter

CMV

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFS6-mVenus

Plasmid

#185806

Purpose

NDUFS6 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFS6 (NDUFS6 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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TagBFP2-FKBP-INPP5ED477N

Plasmid

#202738

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

TagBFP2-FKBP-INPP5ED477N (INPP5E, FKBP1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA7-mVenus

Plasmid

#185647

Purpose

NDUFA7 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA7 (NDUFA7 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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Myc-Nix-MaoB

Plasmid

#100757

Purpose

Mammalian expression of mitochondria targetted Nix

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Nix-MaoB (BNIP3L Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

TM domain replaced by MaoB

Promoter

CMV

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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Myc-Nix-CytoB5

Plasmid

#100756

Purpose

Mammalian expression of ER targeted Nix

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Nix-CytoB5 (BNIP3L Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

TM domain replaced by CytoB

Promoter

CMV

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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HOXB6 (human) FLAG pMSCV

Plasmid

#8534

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB6 (HOXB6 Human)

Use

Retroviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

contains improved ribosomal binding site and FLAG…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
EGFP-Mm_MyoGEF(WT)

Plasmid

#136325

Purpose

Mammalian expression of mouse MyoGEF-WT with N-terminal EGFP

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

PLEKHG6 (Plekhg6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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TFORF3278

Plasmid

#144754

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

MEOX1 (MEOX1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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mouse ZNG1

Plasmid

#197399

Purpose

expresses tagless mouse ZNG1 in the pHIS vector (inserted at NdeI-BamHI)

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

ZNG1 (Cbwd1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pSUMO mouse METAP1

Plasmid

#197395

Purpose

Expresses SUMO-tagged mouse METAP1

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

METAP1 (Metap1 Mouse)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA10-mVenus

Plasmid

#185648

Purpose

NDUFA10 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA10 (NDUFA10 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-NDUFB6-mVenus

Plasmid

#185652

Purpose

NDUFB6 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFB6 (NDUFB6 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEGFP-NDUFS3-mVenus

Plasmid

#185649

Purpose

NDUFS3 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFS3 (NDUFS3 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-NDUFA2-mVenus

Plasmid

#185645

Purpose

NDUFA2 Complex I sub-unit with C-terminus mVenus tag

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA2 (NDUFA2 Human)

Use

Tags

mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-NDUFA10-FKBP-HA

Plasmid

#185810

Purpose

NDUFA10 subunit of Complex I fused with C-terminal FKBP and HA epitope

Depositor

Dipayan Chaudhuri

Article

Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

Insert

NDUFA10 (NDUFA10 Human)

Use

Lentiviral

Tags

FKBP and HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-Mm_MyoGEF(T575A)

Plasmid

#136328

Purpose

Mouse MyoGEF-T575A mutant in Gateway pENTR

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

PLEKHG6 (Plekhg6 Mouse)

Use

Gateway entry vector

Tags

Expression

Mutation

T575A

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV5b HA Irx5 deltaIrot

Plasmid

#24995

Depositor

Benoit Bruneau

Article

Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

Insert

Irx5 (Irx5 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletion of Iro box (aa 314-327), 19 aa upstream …

Promoter

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pCMV5b HA Irx5 deltaC

Plasmid

#24996

Depositor

Benoit Bruneau

Article

Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

Insert

Irx5 (Irx5 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Deletes everything after Hox Domain (aa 112-177).…

Promoter

Available since

Aug. 2, 2010

Availability

Academic Institutions and Nonprofits only
pLenti-Mito-Car-GECO

Plasmid

#100765

Purpose

Lentiviral tet-inducible expression of mito-targeted genetically encoded Ca2+-indicators for optical imaging

Depositor

Joseph Gordon

Article

Mughal et al Cell Death Differ. 2018 Mar 6. pii: 10.1

Insert

Lenti-Mito-Car-GECO

Use

Lentiviral

Tags

a duplex of the mitochondrial targeting signal of…

Expression

Mammalian

Mutation

R-GECO1: E163V/I166V/V174T/M176I/F222I/A302P

Promoter

CMV

Available since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pGS_128

Plasmid

#160651

Purpose

Expresses Human APOE3 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e3 (APOE Human)

Use

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e3 allele

Promoter

pZ estradiol inducible promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR-BLINK2

Plasmid

#117075

Purpose

BLINK2 gene in pDONR vector for Gateway cloning system

Depositor

Anna Moroni

Article

Alberio et al Nat Methods. 2018 Nov;15(11):969-976. do

Insert

BLINK2

Use

Gateway cloning system

Tags

Expression

Mutation

glutamine 112 changed into aspartate (original LO…

Promoter

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGS_101

Plasmid

#160541

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Use

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

GAL1

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGS_107

Plasmid

#160650

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of the yeast Gal promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Use

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

GAL1

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGS_129

Plasmid

#160652

Purpose

Expresses Human APOE4 with a yeast secretory signal peptide under the control of a beta-estradiol inducible promoter

Depositor

Susan Lindquist, Li-Huei Tsai

Article

Sienski et al Sci Transl Med. 2021 Mar 3;13(583). pii:

Insert

Apolipoprotein E e4 (APOE Human)

Use

Tags

Kar2 signal sequence

Expression

Yeast

Mutation

e4 allele

Promoter

pZ estradiol inducible promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-INPP5E-CAAX

Plasmid

#202746

Purpose

express plasma membrane targeted fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:INPP5E(214-644):HRAS(172-189) (HRAS, INPP5E Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCW57.1 blast OFF 3xflag IREB2

Plasmid

#169889

Purpose

Express 3x flag IREB2, suppressible by doxycycline addition

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

IREB2 (IREB2 Human)

Use

Lentiviral; Doxycycline mediated suppression

Tags

3x Flag

Expression

Mammalian

Mutation

Silent mutations in sgIREB2_1 and sgIREB2_2 targe…

Promoter

TRE

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pInducer10b-EGFP-KRAS-WT

Plasmid

#164935

Purpose

Expresses EGFP cDNA and KRAS wild type cDNA in mammalian cells

Depositor

Ji Luo

Article

Carver et al PLoS One. 2014 Aug 5;9(8):e103836. doi:

Inserts

Enhanced green fluorescent protein
KRAS proto-oncogene, GTPase (KRAS) (KRAS Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Ubc Promoter and Ubc promoter

Available since

May 27, 2021

Availability

Academic Institutions and Nonprofits only

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His-PHD2-181-426

Plasmid

#223551

Purpose

His-tagged human PHD2 catalytic domain with TEV cleavage site between His and PHD2 for PHD2 purification

Depositor

Somshuvra Mukhopadhyay

Article

Gurol et al Proc Natl Acad Sci U S A. 2024 Jun 25;12

Insert

PHD2 (EGLN1 Human)

Use

Tags

6x His

Expression

Bacterial

Mutation

Deleted amino acids 1-180.

Promoter

trc

Available since

Sept. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1 ISCU D71A

Plasmid

#169880

Purpose

Expresses ISCU iron-sulfur cluster trapping mutant (dominant negative)

Depositor

Richard Possemato

Article

Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

Insert

ISCU (ISCU Human, Synthetic)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Codon Optimized, D71A iron-sulfur cluster trappin…

Promoter

TRE

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI_Hu_ECT2(WT)

Plasmid

#136330

Purpose

Lentiviral expression of human ECT2 WT

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ECT2 (ECT2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEFL GAL4dbd-KLF4 1-329

Plasmid

#140146

Purpose

GAL4-DNA binding domain fusion protein with the 329 N-terminal amino acids of human KLF4

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 Kruppel factor (KLF4 Human)

Use

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 330 to 479

Promoter

EF1a

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pQCXIP GFP-MOSPD2 W201E

Plasmid

#186471

Purpose

Expression of human MOSPD2 (mutant W201E, unable to bind lipid droplets) fused to EGFP in mammalian cells

Depositor

Catherine Tomasetto

Article

Zouiouich et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

W201E (no more binding to lipid droplets)

Promoter

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCEFL GAL4dbd-KLF4 1-156

Plasmid

#140147

Purpose

GAL4-DNA binding domain fusion protein with the 156 N-terminal amino acids of human KLF4 (activation domain)

Depositor

Ramiro Iglesias-Bartolome

Article

Yuan et al Nat Commun. 2020 Mar 19;11(1):1472. doi:

Insert

KLF4 aa 1-156 (KLF4 Human)

Use

Tags

GAL4dbd

Expression

Mammalian

Mutation

Deleted amino acids 157 to 479

Promoter

EF1a

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUMO mouse METAP1 1-79

Plasmid

#197397

Purpose

Expresses SUMO-tagged mouse METAP1, residues 1-79

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

METAP1 1-79 (Metap1 Mouse)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pSUMO mouse METAP1 1-59

Plasmid

#197396

Purpose

Expresses SUMO-tagged mouse METAP1, residues 1-59

Depositor

David Giedroc

Article

Weiss et al Cell. 2022 Jun 9;185(12):2148-2163.e27.

Insert

METAP1 1-59 (Metap1 Mouse)

Use

Tags

SUMO

Expression

Bacterial

Mutation

Promoter

Available since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pLENTI_Hu_ECT2(T327D)

Plasmid

#136332

Purpose

Lentiviral expression of human ECT2_T327D

Depositor

Marcos Malumbres, Guillermo de Cárcer

Article

de Carcer et al Nat Med. 2017 Aug;23(8):964-974. doi: 10

Insert

ECT2 (ECT2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

T327D

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-2TKN-bp1

Plasmid

#133428

Purpose

Human BP1 fragment of SREBF1 in vector for bacterial expression of GST fusion protein.

Depositor

Gerhard Wagner

Article

Yang et al Nature. 2006 Aug 10;442(7103):700-4. doi

Insert

SREBF1 (SREBF1 Human)

Use

Tags

Expression

Bacterial

Mutation

SREBP1a amino acids 1-50 fused to GST

Promoter

Available since

Jan. 6, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSFDuet-FBXL5-SKP1

Plasmid

#226620

Purpose

Co-expression of FBXL5-SKP1 complex in E.coli

Depositor

Ning Zheng

Article

Wang et al Mol Cell. 2020 Apr 2;78(1):31-41.e5. doi

Inserts

FBXL5 (FBXL5 Human)
SKP1 (SKP1 Human)

Use

Tags

GB1

Expression

Bacterial

Mutation

Amino acids 1-198 was deleted, and 420-596 was re…

Promoter

T7

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

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