EGFP-INPP5E-CAAX
(Plasmid
#202746)
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Purposeexpress plasma membrane targeted fluorescent protein-tagged enzyme
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 202746 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP-C1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.06.30.498262v3 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
EGFP-INPP5E-CAAX was a gift from Gerry Hammond (Addgene plasmid # 202746 ; http://n2t.net/addgene:202746 ; RRID:Addgene_202746) -
For your References section:
A novel homeostatic mechanism tunes PI(4,5)P2-dependent signaling at the plasma membrane. Wills RC, Doyle CP, Zewe JP, Pacheco J, Hansen SD, Hammond GRV. J Cell Sci. 2023 Aug 3:jcs.261494. doi: 10.1242/jcs.261494. 10.1242/jcs.261494 PubMed 37534432