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Plasmid#117686PurposeThe plasmids pSpCas9 (BB)-2A-Puro (Addgene, #48139) and eSpCas9 (1.1) (Addgene, #71814) were combined to express both “enhanced specificity” Cas9 and puromycin resistance protein.DepositorInserteSpCas9(1.1)
UseCRISPRTagsPuroR (R166H)ExpressionMammalianMutationK848A, K1003A, R1060APromoterU6Available sinceFeb. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-SialT
Plasmid#87324PurposeTo express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of β-galactosamide α-2,6-sialyltransferaseDepositorInsertST 6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR221 UBR5 ∆UBR
Plasmid#81063PurposeGateway entry vector encoding full length UBR5 W1235L (∆UBR)DepositorInsertUbiquitin protein ligase E3 component N-recognin 5 (UBR5) ∆UBR (UBR5 Human)
UseCloningTagsExpressionMutationW1235L (bp g3704t)PromoterN/AAvailable sinceJan. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC91-pmax-dCas9VP64
Plasmid#48223PurposedCas9VP64 on pmax expression vectorDepositorInsertdCas9(D10A;H840A) fusion with VP64 activation domain
UseCRISPRTagsHA Tag and VP64ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem161a-V5
Plasmid#175149PurposeLentiviral expression of mouse Tmem161a-V5DepositorInsertTmem161a (Tmem161a Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Reep3-V5
Plasmid#175119PurposeLentiviral expression of mouse Reep3-V5DepositorInsertReep3 (Reep3 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV Tmem9-V5
Plasmid#175151PurposeLentiviral expression of mouse Tmem9-V5DepositorInsertTmem9 (Tmem9 Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC84-pCR8-dCas9
Plasmid#48218PurposedCas9 on Gateway donor vector pCR8/GW/TOPO. Note: This is not for expression. It has to be transferred to a gateway destination vector for expressionDepositorInsertdCas9(D10A;H840A)
UseCRISPR; Gateway donorTagsHA TagExpressionMutationD10A;H840APromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHC01
Plasmid#106900PurposeRatiometric gas biosensor plasmid for 3-oxo-C12-HSL sensingDepositorInsertsLasR
Methyl Halide Transferase
Ethylene Forming Enzyme
Red Fluorescent Protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pB2-neo-TET
Plasmid#51287Purposeexpresses the rodent SINE, B2, using the pol III enhancer sequence of the 7SL gene and tagged with the self splicing intron neo cassetteDepositorInsertsine B2
UseTagsneoTET cassette with a tetrahymena self-splicing …ExpressionMammalianMutationPromoterenhancer from 7SL geneAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-hALDOA
Plasmid#210809PurposeFor transient transfectionDepositorInsertALDOA (ALDOA Human)
UseTagsHAExpressionMammalianMutationPromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-GFP-U6ac-ctrl-guides
Plasmid#168240Purpose"neutrophil specific GFP with ubiquitous ctrl sgRNAs"DepositorInsertcontrol sgRNAs
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
J-HARS-4
Plasmid#166529PurposescFv of a human scaffold targeting Histidyl-tRNA synthetase. Antigen coverage aa 1-509 of 509, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-SARS-7
Plasmid#166527PurposescFv of a human scaffold targeting Seryl-tRNA synthetase. Antigen coverage aa 1-514 of 514, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceApril 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tol2-LyzC-RacFRET-U6ac-ctrl guides
Plasmid#168247Purpose"label active Rac; control for neutrophil-specific knockout"DepositorInsertRacFRET
UseZebrafish expressionTagsExpressionMutationPromoterLyzCAvailable sinceMarch 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV V5-LAP2beta
Plasmid#175112PurposeLentiviral expression of V5-tagged mouse TmpoDepositorInsertTmpo (Tmpo Mouse)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEM-NLS-Cre-2A-mCherry-FKF
Plasmid#119868PurposeDonor cassette containing NLS-tagged Cre, a Thosea asigna virus peptide (2A), mCherry and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertNLS-Cre, 2A, mCherry and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
UseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
RSV/hGATA3
Plasmid#83814PurposeMammalian expression of human GATA3DepositorInsertGATA3 (GATA3 Human)
UseTagsExpressionMammalianMutationPromoterRSVAvailable sinceOct. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAsCpf1(TATV)(BB) (pY221)
Plasmid#89354PurposeExpresses humanized AsCpf1 TATV PAM variant and crRNA guideDepositorInsertCpf1 RVR variant
UseCRISPRTagsNLS and NLS-3xHAExpressionMammalianMutationS542R/K548V/N552RPromoterCBhAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAdEasy-hALDOA
Plasmid#210812PurposeTo package adenovirus expressing wild-type aldolaseADepositorInsertALDOA (ALDOA Human)
UseAdenoviralTagsExpressionMutationPromoterCMVAvailable sinceDec. 22, 2023AvailabilityAcademic Institutions and Nonprofits only