Tol2-LyzC-GFP-U6ac-ctrl-guides
(Plasmid
#168240)
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Purpose"neutrophil specific GFP with ubiquitous ctrl sgRNAs"
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 168240 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneR4R3 pDest
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Vector typeCRISPR
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Selectable markersLyzC-GFP
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecontrol sgRNAs
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gRNA/shRNA sequenceagagacggtcgacagtctgcagtgtcgtctctc
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Reference for p3e-U6a-U6c-control-guides used for gateway cloning:
Neutrophil-specific knockout demonstrates a role for mitochondria in regulating neutrophil motility in zebrafish. Zhou W, Cao L, Jeffries J, Zhu X, Staiger CJ, Deng Q. Dis Model Mech. 2018 Mar 28;11(3). pii: dmm.033027. doi: 10.1242/dmm.033027. 10.1242/dmm.033027
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Tol2-LyzC-GFP-U6ac-ctrl-guides was a gift from Qing Deng (Addgene plasmid # 168240 ; http://n2t.net/addgene:168240 ; RRID:Addgene_168240) -
For your References section:
A robust and flexible CRISPR/Cas9-based system for neutrophil-specific gene inactivation in zebrafish. Wang Y, Hsu AY, Walton EM, Park SJ, Syahirah R, Wang T, Zhou W, Ding C, Lemke AP, Zhang G, Tobin DM, Deng Q. J Cell Sci. 2021 Apr 15;134(8). pii: 237799. doi: 10.1242/jcs.258574. Epub 2021 Apr 22. 10.1242/jcs.258574 PubMed 33722979