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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 13,938 results for: LEA

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Showing: 4961 - 4980 of 13938 results

  3. pIA123

    Plasmid
    #234038
    Purpose
    E. coli-C. difficile shuttle vector for CRISPR editing in C. difficile. Pxyl::Cas9-opt Pgdh::sgRNA-cdr_0985. PstI site to add DNA for homology directed repair. MscI and NotI for replacement of sgRNA.
    Depositor
    Craig Ellermeier
    Publication
    Bollinger et al Proc Natl Acad Sci U S A. 2024 Aug 20;12
    Inserts
    Cas9
    sgRNA
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    Pgdh and Pxyl
    Available since
    March 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    234038-plasmid-map-sequence-id-465702

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  4. p15a_AcCas12n

    Plasmid
    #203806
    Purpose
    p15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.
    Depositor
    Quanjiang Ji
    Publication
    Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AcCas12n
    Use
    CRISPR
    Promoter
    rpsl
    Available since
    Aug. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    203806-plasmid-map-sequence-id-405078

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  12. pEF1-His-NEDD8

    Plasmid
    #159322
    Purpose
    Mammalian cell expression of mouse His-NEDD8
    Depositor
    Jianjun Zhao
    Insert
    His-NEDD8 (Nedd8 Mouse)
    Expression
    Mammalian
    Available since
    Sept. 24, 2020
    Availability
    Academic Institutions and Nonprofits only
    159322-plasmid-map-sequence-id-311864

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  17. pOCC7

    Plasmid
    #118876
    Purpose
    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS6 tag, cleavable with 3C
    Depositor
    Marc Bickle, PEPC MPI CBG
    Publication
    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do
    Insert
    NcoI-NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
    Tags
    HIS6, cleavable with 3C protease
    Expression
    Insect
    Promoter
    polH
    Available since
    Jan. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    118876-plasmid-map-sequence-id-232237

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Showing: 4961 - 4980 of 13938 results