We narrowed to 12,176 results for: LEA
-
Plasmid#112289PurposeFluorescently labels human YAP1-2gamma isoform (504 aa) delta5C mutant lacking the last five residues (FLTWL) in the PDZ binding domain at the N-terminus of the proteinDepositorInsertEYFP-YAP1 (YAP1 Human)
UseLentiviralMutationHuman YAP1-2gamma lacking the last 5 Amino acids …PromoterEF-1 alphaAvailable sinceMay 13, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3.1(+)-blueAKAR(T/A)
Plasmid#118471PurposeNegative-control mutant for blueAKAR biosensor.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertblueAKAR(T/A)
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceApril 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR
Plasmid#113162PurposeA donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable markerDepositorInsertSpCas9
TagsFLAGExpressionMammalianMutationD10APromoterCAGAvailable sinceMarch 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-blueCKAR(T/A)
Plasmid#118473PurposeNegative-control mutant for blueCKAR biosensor.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertblueCKAR(T/A)
Tags6xHIS, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluf
Plasmid#106121PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiGluSnFR E25D variant
TagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pNIC-ZB-SPRTN-E112A-FL
Plasmid#110218PurposeExpression in E. coli of full-length SPRTN protein carrying E112A mutation, a catalytically-dead mutant, with His and ZB tags at N-terminus.DepositorInsertSPRTN (SPRTN Human)
TagsHis6 and ZBExpressionBacterialMutationE112A, P296L (see depositor comments below)Available sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FKBP-Venus-173-C
Plasmid#87617PurposeFKBP-tagged C-terminal Venus (YFP) fragment for BiFC.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFKBP-Venus173-C
Tags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianPromoterCMVAvailable sinceMay 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
MAP1LC3A-V1
Plasmid#98569PurposeExpresses MAP1LC3A-V1DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertMAP1LC3A (MAP1LC3A Human)
ExpressionMammalianPromoterCMVAvailable sinceSept. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
stathmin-CFP
Plasmid#86783PurposeMammalian expression of stathmin-CFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertstathmin (STMN1 Human)
TagsECFPExpressionMammalianPromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEM-NLS-BirA-2A-mCherry-SV40pA-FKF
Plasmid#79889PurposeDonor cassette containing HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection geneDepositorInsertHA-tagged NLS-BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene
ExpressionBacterialAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/FLAG-hPMR1-Tev-Bio
Plasmid#80125PurposeExpresses human PMR1 endonuclease with N-terminal FLAG and C-terminal biotinylation tagsDepositorInsertPXDNL-003 (PXDNL Human)
TagsFLAG and biotinylationExpressionMammalianPromoterCMV promoterAvailable sinceAug. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
STT3A pmRFP (663)
Plasmid#61997Purposemammalian expression of nuclear envelope transmembrane proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSTT3A (STT3A Human)
TagsmRFPExpressionMammalianPromoterCMVAvailable sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-FRT-FAK-kinase0
Plasmid#59122PurposeFAK fret control sensor without FERM domain with no linkerDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFocal Adhesion Kinase
TagsFLAG, mCerulean, and mCitrineExpressionMammalianPromoterCMVAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA6/ERGIC2-WT
Plasmid#51479PurposeExpresses ERGIC2-WT in mammalian cellsDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertERGIC2-WT (ERGIC2 Human)
TagsnoneExpressionMammalianMutationnonePromoterCMVAvailable sinceMarch 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEMS1385
Plasmid#29184PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748).DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPle186 (S100B Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSAvailable sinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
PSUPER-1660s(hPot1)
Plasmid#11132DepositorInsertRNAi vector for human protection of telomeres 1(control for 1660) (POT1 Human)
UseRNAiExpressionMammalianMutationN/AAvailable sinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
PSUPER-344s(hPot1)
Plasmid#11134DepositorInsertRNAi vector for human protection of telomeres 1(control for 344) (POT1 Human)
UseRNAiExpressionMammalianMutationN/AAvailable sinceMarch 3, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCAG-MCP-SRSF1-V5-mCherry
Plasmid#235088PurposeSRSF1 mCherry fusion protein with MCP domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpCAG-MCP-SRSF1-V5-mCherry (SRSF1 Human)
ExpressionMammalianPromoterCAGAvailable sinceMay 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-BsmBI_epegRNA_entry-mpknot_(LM1140)
Plasmid#228253PurposepU6 entry plasmid for cloning prime editor mpknot epegRNAs (requires BsmBI or Esp3I digest and ligation of duplexed oligos)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmpknot entry plasmid backbone for epegRNA expression via human U6 promoter
ExpressionMammalianMutationn/aPromoterU6Available sinceApril 23, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLVX--DmrB-DmrB-Ripk3(deltaC)-2A-mCherry-puro
Plasmid#231973PurposeTet inducible expression of DmrB-Ripk3 with mCherry to induce Ripk3 necroptosisDepositorInsertRipk3-deltaC dimerizing construct with bi-cistronic mCherry (Ripk3 Mouse)
UseLentiviralAvailable sinceFeb. 5, 2025AvailabilityAcademic Institutions and Nonprofits onlyCited by