We narrowed to 15,670 results for: LEA

1. pY014 (pcDNA3.1-hMbCpf1)

   Plasmid

   #69986

   Purpose

   Expresses humanized MbCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hMbCpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pWZL N-Myc-hPOT1_deltaOB

   Plasmid

   #166408

   Purpose

   express hPOT1 ΔOB in mammalian cells

   Depositor

   Agnel Sfeir

   Article

   Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

   Insert

   POT1 ΔOB (POT1 Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   ΔOB

   Promoter

   Available Since

   May 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET21-pelB-LaG-42

   Plasmid

   #172758

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-42
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-AktAR2-NES

   Plasmid

   #175011

   Purpose

   Cytosol-targeted AktAR2.

   Depositor

   Jin Zhang

   Article

   Zhou et al Nat Commun. 2020 Nov 30;11(1):6088. doi:

   Insert

   AktAR-NES
   

   Use

   Tags

   6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

   Plasmid

   #182824

   Purpose

   Cre-dependent expression of cytosolic APEX

   Depositor

   Yevgenia Kozorovitskiy

   Article

   Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

   Insert

   APEX.NES-P2A-EGFP
   

   Use

   AAV

   Tags

   EGFP reporter (P2A)

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   May 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EPB104

   Plasmid

   #68369

   Purpose

   contains human U6 promoter

   Depositor

   Eric-Paul Bennett

   Article

   Lonowski et al Nat Protoc. 2017 Mar;12(3):581-603. doi:

   Insert

   U6 promoter (RNU6-1 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   U6

   Available Since

   Feb. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. RhoC FLARE.sc mCer, mVenus - CA

   Plasmid

   #65424

   Purpose

   RhoC CA biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC Q63L

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus, Q63L in RhoC

   Promoter

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

8. RhoC FLARE.sc mCer, mVenus - DN

   Plasmid

   #65423

   Purpose

   RhoC DN biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC T19N

   Depositor

   Klaus Hahn

   Article

   Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

   Insert

   RBD, mCerulean, mVenus, RhoC
   

   Use

   Tags

   Expression

   Bacterial, Insect, and Mamm…

   Mutation

   T153M in mVenus, T19N in RhoC

   Promoter

   Available Since

   Aug. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

9. MSP977

   Plasmid

   #65774

   Purpose

   Human expression vector for SpCas9 D1135E variant: CMV-T7-humanSpCas9(D1135E)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E)-NLS-3XFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   D1135E mutation in Cas9

   Promoter

   CMV & T7

   Available Since

   June 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET21-pelB-LaG-6

    Plasmid

    #172741

    Purpose

    Bacterial expression of HIS-tagged anti-GFP nanobody LaG-6; HIS tag preceded by HRV 3C/PreScission cleavage site.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaG-6
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. CMV/N3IC-HA

    Plasmid

    #47618

    Purpose

    in vitro transcription and translation of HA-tagged Notch3 Intracellular domain

    Depositor

    Urban Lendahl

    Article

    Lardelli et al Mech Dev. 1996 Oct;59(2):177-90.

    Insert

    Notch3 Intracellular domain (Notch3 Mouse)
    

    Use

    In vitro transcription and translation

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codons 1664 (Met) to 2318 (Ala)

    Promoter

    CMV

    Available Since

    Aug. 28, 2013

    Availability

    Academic Institutions and Nonprofits only

12. pHS0663 pET45b(+) KraIscB-1 TwinStrep

    Plasmid

    #176536

    Purpose

    Bacterial expression of KraIscB-1-TwinStrep

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

    Insert

    KraIscB-1
    

    Use

    Tags

    TwinStrep

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. WT (AF20)

    Plasmid

    #21359

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    wild-type sequence*

    Promoter

    Available Since

    Jan. 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21 E2F1

    Plasmid

    #90313

    Purpose

    Expresses human E2F1 in bacteria

    Depositor

    Maria Alvarado-Kristensson

    Article

    Hoog et al FASEB J. 2011 Nov;25(11):3815-27. doi: 1

    Insert

    E2F1 (E2F1 Human)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Aug. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pRSFDuet-1_VNp-LZ-mNeongreen

    Plasmid

    #182392

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusion

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Insert

    VNp-LZ-mNeongreen
    

    Use

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLVH-EF1A-BRD3R

    Plasmid

    #130696

    Purpose

    Lentiviral plasmids encoding human BRD3R

    Depositor

    Kejin Hu

    Article

    Shao et al Nat Commun. 2016 Mar 7;7:10869. doi: 10.

    Insert

    Human BRD3R (BRD3 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pYJ34-PB-CRISPR-BANCR-E1-gRNA

    Plasmid

    #131077

    Purpose

    long non-coding RNA BANCR knock out

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

    Insert

    BANCR Exon1 gRNA (BANCR Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLVX-EF1a-EGFP-RAMP4-IRES-Puromycin

    Plasmid

    #134863

    Purpose

    Expresses EGFP-tagged endoplasmic reticulum marker

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    RAMP4 (SERP1 Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLV U6-sgRNA UbC-tdTomato

    Plasmid

    #106949

    Purpose

    sgRNA cloning cassette using BsmBI/Esp3I enzymes with tdTomato marker

    Depositor

    Albert Edge

    Article

    Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

    Insert

    sgRNA BsmBI/Esp3I cloning site
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1 HA-rnSOX9 ΔC (1-381)

    Plasmid

    #62973

    Purpose

    Expresses HA-tagged rat SOX9 residues 1-383

    Depositor

    Michael Ruppert

    Article

    Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

    Insert

    SOX9 (Sox9 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codes for residues 1-381

    Promoter

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only