We narrowed to 15,836 results for: LEA

1. pVSL10

   Plasmid

   #201182

   Purpose

   Bst polymerase large fragment (BF) F710Y/D598A, His6-tagged, HRV 3C-cleavable

   Depositor

   Jack Szostak

   Article

   Lelyveld et al Science. 2023 Oct 27;382(6669):423-429.

   Insert

   Bst polymerase large fragment (BF) F710Y/D598A
   

   Tags

   HRV 3C protease cleavage site and His6

   Expression

   Bacterial

   Mutation

   F710Y D598A

   Promoter

   T5-lacO

   Available Since

   Sept. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBT313_pR26-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

   Plasmid

   #52546

   Purpose

   Targeting vector for homologous recombination in mouse ES cells; Rosa26 locus

   Depositor

   Liqun Luo

   Article

   Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

   Inserts

   PhiC31o attPx3 (70 bp each)
   Neo
   PhiC31o integrase
   

   Expression

   Mammalian

   Available Since

   Aug. 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pEF Synaptophysin-GCamp6s

   Plasmid

   #188981

   Purpose

   Presynaptic fluorescent Ca2+ indicator

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Synaptophysin (Syp Rat, Synthetic)
   

   Expression

   Mammalian

   Available Since

   Dec. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pcDNA3.1V5-His Snk/Plk2 T239A

   Plasmid

   #22219

   Depositor

   Wafik El-Deiry

   Article

   Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

   Insert

   Snk/Plk2 (PLK2 Human)
   

   Expression

   Mammalian

   Mutation

   single amino acid substitution mutant, threonine …

   Available Since

   Aug. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

5. p2T-CAG-REV1-p2A-GFP-PuroR

   Plasmid

   #153001

   Purpose

   Overexpression of human REV1

   Depositor

   David Liu

   Article

   Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

   Insert

   REV1 (REV1 Human)
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pRSFDuet-1_VNp6-DARPinOFF7

   Plasmid

   #182398

   Purpose

   Bacterial expression of Vesicle Nucleating peptide6-DARPinOFF7 fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp6-DARPinOFF7
   

   Tags

   Vesicle Nucleating peptide 6 (VNp6)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pDR-GFPuniv

   Plasmid

   #46085

   Purpose

   In vivo recombination assay fluorescent reporter

   Depositor

   Raymond Monnat

   Article

   Li et al Nucleic Acids Res. 2012 Mar;40(6):2587-9

   Insert

   DR-GFPuniv reporter
   

   Use

   Recombination reporter plasmid

   Expression

   Mammalian

   Mutation

   E5G and K163R mutations in EGFP relative to wild-…

   Available Since

   May 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMSCV-human-pro-IL-18

   Plasmid

   #214317

   Purpose

   Expression of gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

   Insert

   IL18 (IL18 Human)
   

   Use

   Retroviral

   Tags

   Flag and Myc

   Promoter

   MESV

   Available Since

   Feb. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pY011 (pcDNA3.1-hLb2Cpf1)

   Plasmid

   #69983

   Purpose

   Expresses humanized Lb2Cpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hLb2Cpf1
   

   Use

   CRISPR

   Tags

   NLS-3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. 3×Cherry Dam AID Smash

    Plasmid

    #227374

    Purpose

    For inducible expression and cytosolic localization of Dam

    Depositor

    David Pellman

    Article

    Papathanasiou et al Nature. 2023 Jul;619(7968):184-192. doi:

    Insert

    Dam DNA-adenine methyltransferase
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pZS*11_4enh

    Plasmid

    #149423

    Purpose

    Plasmid harboring four plant enhancers (35S, AB80, Cab-1, and rbcS-E9).

    Depositor

    Stan Fields

    Article

    Jores et al Plant Cell. 2020 May 14. pii: tpc.20.001

    Inserts

    rbcS-E9 enhancer
    35S core enhancer
    Cab-1 enhancer
    AB80 enhancer
    

    Tags

    T120>A

    Expression

    Bacterial

    Available Since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET15-His-∆NGlpG

    Plasmid

    #155131

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    insert codes for GlpG residues 87-276

    Promoter

    T7

    Available Since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Cas12j-8-puro

    Plasmid

    #194966

    Purpose

    The plasmid expresses human codon-optimized Cas12j-8 and puromycin resistence gene.

    Depositor

    Yongming Wang

    Article

    Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. RhoC FLARE.sc mCer, mVenus - wt

    Plasmid

    #65071

    Purpose

    RhoC biosensor single chain; mCerulean-tagged ROCK-RBD, mVenus-tagged RhoC

    Depositor

    Klaus Hahn

    Article

    Zawistowski et al PLoS One. 2013 Nov 5;8(11):e79877. doi:

    Insert

    RBD, mCerulean, mVenus, RhoC
    

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    T153M in mVenus

    Promoter

    CMV, p10

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

15. FRB_LD 0_CS G_tTA-BFP

    Plasmid

    #58876

    Purpose

    MESA target chain with Rapamycin FRB ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusion

    Depositor

    Joshua Leonard

    Article

    Daringer et al ACS Synth Biol. 2014 Mar 11.

    Insert

    MESA target chain with FRB ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
    

    Use

    AAV

    Tags

    EBFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTU424

    Plasmid

    #178983

    Purpose

    pACYC containing Candidatus “Scalindua brodae” TPR-CHAT (codon-optimized)

    Depositor

    Stan Brouns

    Article

    van Beljouw et al Science. 2021 Sep 17;373(6561):1349-1353

    Insert

    TPR-CHAT
    

    Tags

    His-tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pETDuet-1_VNp-mNeongreen_CydAB-mCherry

    Plasmid

    #182391

    Purpose

    Bacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusions

    Depositor

    Dan Mulvihill

    Article

    Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

    Inserts

    VNp-mNeongreen-His
    cydAB-mCherry
    

    Tags

    Vesicle Nucleating peptide (VNp)

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21-pelB-LaM-3

    Plasmid

    #172769

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-3
    

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPPEM

    Plasmid

    #162467

    Purpose

    Destination Expression vector for monocot plant primer editing

    Depositor

    Adam Bogdanove

    Article

    Wang et al Front Genome Ed. 2021 Mar 4;3:617553. do

    Insert

    Cas9H840A
    

    Tags

    MLV reverse transcriptase

    Expression

    Plant

    Mutation

    Change histidine 840 to Alanine

    Promoter

    maize Ubi

    Available Since

    June 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNAintron-CHIKV-Envelope protein

    Plasmid

    #215700

    Purpose

    Produces the Chikungunya virus envelope protein E3-E2-6K-E1

    Depositor

    Melinda Brindley

    Article

    Reyes Ballista et al J Virol. 2024 Jul 15:e0077524. doi: 10.1

    Insert

    CHIKV E3-E2-6K-E1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 1, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge