We narrowed to 12,207 results for: LEA

Showing: 4961 - 4980 of 12207 results

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pEGFP/golgin-160/3DE

Plasmid

#180631

Purpose

Expression of caspase-resistant golgin-160

Depositor

Carolyn Machamer

Publication

Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

golgin-160/3DE (GOLGA3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D59E, D139E, D311E

Available since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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pNOC_dCas9_sgRNA NC

Plasmid

#176259

Purpose

pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and spacer sequence on sgRNA is replaced by the type IIS restriction site for endonuclease BaeI and

Depositor

John van der Oost

Publication

Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

dead spCas9

Use

CRISPR, Luciferase, and Synthetic Biology ; Expre…

Tags

luciferase

Mutation

H840A and D10A mutations on spCas9 to inactivate …

Available since

Oct. 28, 2021

Availability

Academic Institutions and Nonprofits only

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pCjedCas9

Plasmid

#172216

Purpose

Expresses catalytically inactive Campylobacter jejuni Cas9 (CjedCas9) in bacterial cells

Depositor

John van der Oost

Publication

Saha et al Sci Adv. 2020 Jun 17;6(25):eaaz4849. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

catalytic mutant of Cas9 endonuclease from the Campylobacter jejuni Type II CRISPR/Cas system

Tags

Histag

Mutation

changed Asp 8 to Ala and His 559 to Ala

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_RBMX-eA3A-UdgX-HF-nCas9

Plasmid

#163548

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Publication

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

RBMX-eA3A-UdgX-HF-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pRG714_lexO-AscI_LexA-TF_URA3MX

Plasmid

#154822

Purpose

Inducible expression of AscI endonuclease in S. cerevisiae

Depositor

Lorraine Symington

Publication

Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

Inserts

AscI endonuclease
LexA-ER-B112

Tags

FLAG and NLS

Expression

Yeast

Promoter

ACT1 and lexO_4-CYC1_core

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pGBW-m4133607

Plasmid

#152942

Purpose

Mammalian expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133721

Plasmid

#152946

Purpose

Mammalian expression plasmid for SARS-CoV-2 3C-like proteinase

Depositor

Ginkgo Bioworks, Benjie Chen

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 3C-like proteinase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133554

Plasmid

#152860

Purpose

Mammalian expression plasmid for SARS-CoV-2 M (membrane)

Depositor

Ginkgo Bioworks, Benjie Chen

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4133371

Plasmid

#152780

Purpose

Mammalian expression plasmid for SARS-CoV-2 helicase

Depositor

Ginkgo Bioworks, Benjie Chen

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 helicase (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134293

Plasmid

#152658

Purpose

Mammalian expression plasmid for SARS-CoV-2 ORF3a protein

Depositor

Ginkgo Bioworks, Benjie Chen

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134114

Plasmid

#152632

Purpose

Mammalian expression plasmid for SARS-CoV-2 nsp9

Depositor

Ginkgo Bioworks, Benjie Chen

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 nsp9 (ORF1ab Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134375

Plasmid

#152788

Purpose

Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;HA

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 30, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4134545

Plasmid

#152594

Purpose

Mammalian expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;V5

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

July 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pGBW-m4134342

Plasmid

#152111

Purpose

Mammalian expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2)

Tags

Cleavable TEV;3xFlag

Expression

Mammalian

Mutation

wt

Promoter

CMV

Available since

May 20, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046418

Plasmid

#149029

Purpose

Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;3xFLAG

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

May 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046475

Plasmid

#145531

Purpose

Yeast Expression plasmid for SARS-CoV-2 N (nucleocapsid)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 N (nucleocapsid) (N Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;StrepII

Expression

Yeast

Mutation

wt

Promoter

pGAL1

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGBW-m4046476

Plasmid

#145512

Purpose

Yeast Expression plasmid for SARS-CoV-2 E (envelope)

Depositor

Ginkgo Bioworks, Benjie Chen

Insert

SARS-CoV-2 E (envelope) (E Severe acute respiratory syndrome coronavirus 2, Budding Yeast)

Tags

Cleavable thrombin;6xHIS

Expression

Yeast

Mutation

wt

Promoter

pTEF1

Available since

April 29, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2+8 Flag2AUF1(p40)

Plasmid

#135945

Purpose

Expresses AUF1p40 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Publication

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Tags

Flag2

Expression

Mammalian

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2+8 Flag2AUF1(p42)

Plasmid

#135946

Purpose

Expresses AUF1p42 in mammalian cells. Can also be used to generate mRNA for zebrafish expression.

Depositor

Yimon Aye

Publication

Poganik et al FASEB J. 2019 Nov 1:fj201901930R. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

heterogeneous nuclear ribonucleoprotein D (HNRNPD Human)

Tags

Flag2

Expression

Mammalian

Promoter

CMV and SP6

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-mIRFP670nuc-TEV-Xph20-ETWV

Plasmid

#133044

Purpose

Expresses Xph20-ETWV in mammalian cells with nuclear miRFP670 as reporter

Depositor

Matthieu Sainlos

Publication

Rimbault et al Nat Commun. 2019 Oct 4;10(1):4521. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Xph20-ETWV

Tags

NLS, TEV protease + TEV cleavage site, and miRFP6…

Expression

Mammalian

Mutation

S63K

Promoter

CAG

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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