Addgene

We narrowed to 14,528 results for: LEA

Showing: 4961 - 4980 of 14528 results

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GLRX-roGFP2 in pT3TS-Dest

Plasmid

#194297

Purpose

In vitro transcription of the redox sensor GLRX-roGFP2 from the T3 promoter. Parton lab clone KRK

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

GLRX-roGFP2

Use

In vitro transcription of mrna

Tags

Expression

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-LZ_CydAB-mNeongreen

Plasmid

#182393

Purpose

Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper and cydAB--mNeongreen fusions

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Inserts

VNp-LZ
CydAB-mNeongreen

Use

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pME-p3E-GLRX-roGFP2

Plasmid

#194288

Purpose

Multisite gateway entry clone for expression of the redox probe GLRX-roGFP2. Parton lab clone KQF

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

GLRX-roGFP2

Use

Multisite gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLV sgRNA-Hic1 GFP

Plasmid

#106953

Purpose

Lentivirus encoding sgRNA targeting murine Hic1. Includes GFP marker.

Depositor

Albert Edge

Article

Lenz et al Front Cell Dev Biol. 2019 Feb 19;7:14. d

Insert

anti-Hic1 sgRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pPBpuro-mCherry-HRasCT

Plasmid

#186704

Purpose

Encoding membrane-targeted mCherry

Depositor

Michiyuki Matsuda

Article

Hino et al Dev Cell. 2022 Oct 10;57(19):2290-2304.e

Insert

mCherry-HRasCT

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.040

Plasmid

#184974

Purpose

Test effect of extending a1/a2 on ADE2 editing rates in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v2

Use

Tags

Expression

Yeast

Mutation

ADE2 donor P272stop, a1/a2 length extended to 27 …

Promoter

Gal7

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.108

Plasmid

#184977

Purpose

Express -Eco1 LYP1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 12

Use

Tags

Expression

Yeast

Mutation

LYP1 donor E27stop

Promoter

Gal7

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.109

Plasmid

#184978

Purpose

Test effect of extending a1/a2 on LYP1 editing rates in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, LYP1 E27X, a1/a2 length: 27 v1

Use

Tags

Expression

Yeast

Mutation

LYP1 donor E27stop, a1/a2 length extended to 27 bp

Promoter

Gal7

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.107

Plasmid

#184976

Purpose

Test effect of extending a1/a2 on CAN1 editing rates in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 27 v1

Use

Tags

Expression

Yeast

Mutation

CAN1 donor G444stop, a1/a2 length extended to 27 …

Promoter

Gal7

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.106

Plasmid

#184975

Purpose

Express -Eco1 CAN1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, CAN1 G444X, a1/a2 length: 12

Use

Tags

Expression

Yeast

Mutation

CAN1 donor G444stop

Promoter

Gal7

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.111

Plasmid

#184980

Purpose

Test effect of extending a1/a2 on TRP2 editing rates in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 27 v1

Use

Tags

Expression

Yeast

Mutation

TRP2 donor E64stop, a1/a2 length extended to 27 bp

Promoter

Gal7

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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PRE-Hsp70BbCas9_1.0

Plasmid

#190795

Purpose

PRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning process

Depositor

Omar Akbari

Article

Warsinger-Pepe et al G3 (Bethesda). 2023 Apr 11;13(4):jkad024

Insert

PRE-Hsp70Bb-Cas9-T2A-eGFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.037

Plasmid

#184966

Purpose

Express -Eco1 dead RT and ncRNA in yeasst

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT

Use

Tags

Expression

Yeast

Mutation

Human codon optimized RT, YXDD catalytic region m…

Promoter

Gal7

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.112

Plasmid

#184981

Purpose

Express -Eco1 FAA1 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 12

Use

Tags

Expression

Yeast

Mutation

FAA1 donor P233stop

Promoter

Gal7

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.113

Plasmid

#184982

Purpose

Test effect of extending a1/a2 on FAA1 editing rates in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, FAA1 P233X, a1/a2 length: 27 v1

Use

Tags

Expression

Yeast

Mutation

FAA1 donor P233stop, a1/a2 length extended to 27 …

Promoter

Gal7

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.002

Plasmid

#184972

Purpose

Express -Eco1 ADE2 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 12

Use

Tags

Expression

Yeast

Mutation

ADE2 donor P272stop

Promoter

Gal7

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.110

Plasmid

#184979

Purpose

Express -Eco1 TRP2 editing ncRNA and gRNA

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, TRP2 E64X, a1/a2 length: 12

Use

Tags

Expression

Yeast

Mutation

TRP2 donor E64stop

Promoter

Gal7

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.039

Plasmid

#184973

Purpose

Test effect of extending a1/a2 on ADE2 editing rates in yeast

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1 editing ncRNA and gRNA, ADE2 P272X, a1/a2 length: 27 v1

Use

Tags

Expression

Yeast

Mutation

ADE2 donor P272stop, a1/a2 length extended to 27 …

Promoter

Gal7

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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hsLEMD2-pX330

Plasmid

#179511

Purpose

Encodes gRNA for human LEMD2

Depositor

Yasushi Hiraoka

Article

Haraguchi et al Commun Biol. 2022 Jan 20;5(1):78. doi: 1

Insert

gRNA for LEMD2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pNHT7

Plasmid

#186753

Purpose

A basic cloning vector for inserting genes with 5' and 3' UTRs for later subcloning via Golden Gate assembly.

Depositor

Bo Wang

Article

Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

Type

Empty backbone

Use

Synthetic Biology; Cloning backbone

Tags

Expression

Mutation

Promoter

T7

Available since

Oct. 24, 2022

Availability

Academic Institutions and Nonprofits only

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