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pcDNA3.1-2xFLAG-2xSTREP-CCND1(P287L)

Plasmid

#172646

Purpose

Expresses 2xFLAG-2xSTREP-tagged cyclin D1(P287L) in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Pro287Leu

Promoter

CMV

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-2xFLAG-2xSTREP-CCND1-Puro

Plasmid

#172634

Purpose

Retroviral vector for the constitutive, near-physiological expression of 2xFLAG-2xSTREP-tagged cyclin D1 and a puromycin resistance marker in mammalian cells

Depositor

Michele Pagano

Article

Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

Insert

CCND1 (CCND1 Human)

Use

Retroviral

Tags

2xFLAG-2xSTREP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDY153: Flag(SUZ12) WT HDR plasmid

Plasmid

#170793

Purpose

WT homology directed repair plasmid for performing genome-editing on the SUZ12 Locus

Depositor

Thomas Cech

Article

Youmans et al Mol Cell. 2021 Feb 4;81(3):488-501.e9. d

Insert

SUZ12 (SUZ12 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1b_nsp10-6His-3xFlag (SARS-CoV-2)

Plasmid

#169162

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp10 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

nsp10-6His-3xFlag (ORF1ab Synthetic, SARS-CoV-2)

Use

Tags

6His-3xFlag

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pBIG1a_3xFlag-6His-nsp14 (SARS-CoV-2)

Plasmid

#169163

Purpose

Baculoviral transfer vector to express SARS-CoV-2 nsp14 in insect cells

Depositor

John Diffley

Article

Canal et al Biochem J. 2021 Jul 16;478(13):2445-2464

Insert

3xFlag-6His-nsp14 (ORF1ab Synthetic, SARS-CoV-2)

Use

Tags

3xFlag-6His

Expression

Insect

Mutation

Codon optimised for insect cells (Spodoptera frug…

Promoter

Polyhedrin

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT I261A

Plasmid

#153476

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT I261A

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, I261A

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT S333A

Plasmid

#153477

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT S333A

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, S333A

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT deltaALAS

Plasmid

#153471

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT deltaALAS

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, deletion of amino aci…

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT delta24-36

Plasmid

#153472

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT delta24-36

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, deletion of amino aci…

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT E102A

Plasmid

#153473

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT E102A

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, E102A

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT M122A

Plasmid

#153474

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT M122A

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, M122A

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pCEP4-FLAG-TAPBPR-tapasinCT G212A

Plasmid

#153475

Purpose

Mammalian expression of FLAG-tagged TAPBPR with tapasin CT G212A

Depositor

Erik Procko

Article

McShan et al Nat Commun. 2021 May 26;12(1):3174. doi:

Insert

TAPBPR (TAPBPL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

C terminal tail of tapasin, G212A

Promoter

CMV

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pNIA-CEN-FLAG-LANS1-Set2

Plasmid

#122002

Purpose

FLAG-LANS-Set2: FLAG-tagged LANS1-Set2 for light control of Set2 localization in yeast

Depositor

Brian Kuhlman

Article

Lerner et al Genome Res. 2020 Nov;30(11):1605-1617. d

Insert

histone methyltransferase SET2 (SET2 Budding Yeast)

Use

Synthetic Biology

Tags

FLAG

Expression

Yeast

Mutation

Set2 point mutations K538G, K539S, R549G, K550S, …

Promoter

ADH1

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 T538N

Plasmid

#167181

Purpose

puromycin resistant lentiviral vector for expression of PAK5 T538N

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 T538N

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-3xFLAG-Puro PAK5 V593I

Plasmid

#167179

Purpose

puromycin resistant lentiviral vector for expression of PAK5 V593I

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 V593I

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti--Puro-3xFLAG PAK5 S364L

Plasmid

#167168

Purpose

puromycin resistant lentiviral vector for expression of PAK5 S364L

Depositor

Christin Burd

Article

LaPak et al Oncotarget. 2018 May 22;9(39):25386-2540

Insert

PAK5 S364L

Use

Lentiviral

Tags

3x flag

Expression

Mammalian

Mutation

Promoter

UBC promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-EGFP-T2A-RPL22-1xFlag

Plasmid

#170323

Purpose

Expresses FLAG and EGFP tagged RPL22

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG R394E

Plasmid

#169954

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (R394E)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

R394E

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8 Ub-S297-FLAG

Plasmid

#169958

Purpose

Mammalian expression of the CARD8 C-terminus fused to ubiquitin (co-translationally processed)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG and Ub

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 LIC 6A CARD8-FLAG L368G

Plasmid

#169952

Purpose

Mammalian expression of CARD8-FLAG with a site III mutation (L368G)

Depositor

Hao Wu

Article

Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

Insert

CARD8 (CARD8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

L368G

Promoter

CMV

Available since

May 11, 2021

Availability

Academic Institutions and Nonprofits only

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