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Plasmid#84140Purposetarget miRNA771 for destruction in Arabidopsis thalianaDepositorInsertSTTM771
UseTagsExpressionPlantMutationPromoter2x35SAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB432
Plasmid#68697PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterMpHSP17.8A1 promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTBL3428 MTK234-LacO-BtoApegRNA
Plasmid#226669PurposeMTK234 part containing lac-inducible variant of the hU6 promoter driving a B to A pegRNADepositorInserthU6-LacOS-BtoApegRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-LacOSAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTBL3331 MTK234-TetO-AtoBpegRNA
Plasmid#226668PurposeMTK234 part containing tet-inducible variant of the hU6 promoter driving an A to B pegRNADepositorInserthU6-TetO-AtoBpegRNA
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterhU6-TetOAvailable sinceNov. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet-1:ChrA-C63S
Plasmid#225080PurposeExpresses precursor peptide ChrA-C63S in E.coliDepositorInsertChrA-C63S
UseTagsHis6ExpressionBacterialMutationC63SPromoterT7Available sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-yhaM
Plasmid#207650PurposeOverexpression of native L-cysteine desulfidase in E. coli MG1655DepositorInsertyhaM
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC-decR
Plasmid#207653PurposeOverexpression of L-cysteine desulfidase transcriptional regulator in E. coli MG1655DepositorInsertdecR
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTE5418_pMBP-GlcR
Plasmid#221192PurposeExpression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.DepositorInsertglcR (pden4400 from Paracoccus denitrificans)
UseTags10x His tag with E. coli maltose binding protein …ExpressionBacterialMutationPromoterT7 promoter and T7 promoter-lacOAvailable sinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB402SC
Plasmid#224552PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes C-terminal Twin-Strep-HA tags. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneUseTagsTwin-Strep-HAExpressionPlantMutationPromoter2 X 35SAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGWB402SN
Plasmid#224551PurposeEmpty, Gateway-compatible expression vector for transient transformation of N. benthamiana leaves. Vector backbone encodes an N-terminal HA-Twin-Strep tag. Duplicated 35S promoter and 35S terminator.DepositorTypeEmpty backboneUseTagsHA-Twin-StrepExpressionPlantMutationPromoter2 X 35SAvailable sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC2861 (pSpy1C-PgyrA_ffluc)
Plasmid#218511Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseTagsExpressionMutationdeletion of Plac_mrfp cassettePromoterPgyrAAvailable sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEC3105 (pSpy1C-PxylS2_ffluc)
Plasmid#218512Purposereplicative E. coli-S. pyogenes shuttle plasmid, constitutive expression of firefly luciferase reporterDepositorInsertffluc
UseLuciferaseTagsExpressionMutationdeletion of Plac_mrfp cassettePromoterPxylS2Available sinceSept. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNP1830
Plasmid#222728PurposepNP561 - T5 Target with T7 Inducible IS621 RecombinaseDepositorInsertIS621 Recombinase
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr
Plasmid#218766Purposeencodes EcTyrRS-VSMA* and EcTyr-tRNA-TAGDepositorInsertEcTyrRS-VSMA*
UseTagsExpressionBacterialMutationY37V, D167G, D182S, F183M, L186A, D265RPromotertacIAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB3474
Plasmid#215370PurposeCDS of CPH from N. nambi codon optimized for N.benthamiana. This gene is the final step and recycling of the bioluminiscente pathwayDepositorInsertCPH
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB3473
Plasmid#215246PurposeCDS of Luz gene from N. nambi codon optimized for N.benthamiana. This gene generate the luminiscence of the bioluminiscente pathwayDepositorInsertLuz
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
GB3472
Plasmid#215245PurposeCDS of H3H from N. nambi codon optimized for N.benthamiana. This gene is the second step of the bioluminiscente pathwayDepositorInsertH3H
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceJune 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-h.Rhno1 Plk1mut7A-C-PAMmut-Myc-DDK-P2A-Puro
Plasmid#211616PurposeRhno1 Plk1mut7ADepositorInserthRHNO1 (RHNO1 Human)
UseTagsmycExpressionMammalianMutationS51A, T52A, S141A, S162A, S163A, S164A, S178APromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLP-pTac-Tn5-chlorR
Plasmid#199994PurposeLanding Pad with T7 RNAP circuit variant T15. No seed promoter. pTac-Tn5 transposase. In dap auxotrophic conjugation donor. This material contains 2 plasmids. Look below for more information.DepositorInsertLanding Pads
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACYC Destination
Plasmid#207675PurposeTrio “destination vectors” which harbor an operon for constitutive expression of the repressors for each inducible promoter (CymR, PhlF, and VanR for cumate, DAPG and vanillate, respectively).DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterCymRC, PhlF, VanCCAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only