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Plasmid#202585PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein with the K3A and K4A mutations in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
UseTags6x His and TEV protease cleavage siteExpressionBacterialMutationChanged ORF1p Lysine 3 to Alanine, changed ORF1p …PromoterAvailable sinceNov. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEX SYT9 C2A D197,199N
Plasmid#195704PurposeBacterial expression plasmid encoding GST-tagged SYT9 C2A domain with D197, 199N mutationsDepositorInsertSyt9 (Syt5 Mouse)
UseTagsGSTExpressionBacterialMutationencodes amino acids 124-211 of mouse SYT9 with D1…PromoterAvailable sinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
MLKP2delKASH
Plasmid#130838PurposeExpression of plant LINC fluorescent fusion protein for cell biologyDepositorInsertMLKP2
UseTagseGFP-FLAG-HAExpressionPlantMutationDeletion of KASH domain, addition of three alani…PromoterCaMV 35SAvailable sinceSept. 28, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAG-AaCas12b(Q119F/E475R/E758R)-T2A-EGFP
Plasmid#188272PurposeMammalian expression, Genome editingDepositorInsertAaCas12b(Q119F/E475R/E758R)
UseCRISPRTagsExpressionMammalianMutationnonePromoterCAGAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-C-TEV-HAT
Plasmid#202597PurposeExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site (ENLYFQG) and poly-histidine a…ExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-N-TEV-HAT
Plasmid#202598PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site (ENLYFQG) and poly-histidine a…ExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOET3-C-TEV-Twin-Strep
Plasmid#203324PurposeGenerate the baculovirus to express N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-tagged protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK) in insect cellsDepositorTypeEmpty backboneUseTagsTEV cleavable site (ENLYFQG) and Twin-strep-tagExpressionInsectMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-DIO-basic
Plasmid#196882PurposeUsed for preparation of double-floxed inverse ORF vectorsDepositorTypeEmpty backboneUseCre/Lox; Dre/roxTagsExpressionMammalianMutationPromoterAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NpM-DmPAN2_468-1241_Y
Plasmid#148095PurposeBacterial Expression of DmPAN2_468-1241DepositorInsertDmPAN2_468-1241 (PAN2 Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN2-dsRNAres_Y
Plasmid#148071PurposeInsect Expression of DmPAN2-dsRNAresDepositorInsertDmPAN2-dsRNAres (PAN2 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN3-E679LG683V-dsRNAres_Y
Plasmid#148081PurposeInsect Expression of DmPAN3-E679LG683V-dsRNAresDepositorInsertDmPAN3-E679LG683V-dsRNAres (CG11486 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN3-F658EL622EL665EW707AG711E-dsRNAres_Y
Plasmid#148082PurposeInsect Expression of DmPAN3-F658EL622EL665EW707AG711E-dsRNAresDepositorInsertDmPAN3-F658EL622EL665EW707AG711E-dsRNAres (CG11486 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN3-FLLWGYHRtoEEEAEAAA-dsRNAres_Y
Plasmid#148083PurposeInsect Expression of DmPAN3-FLLWGYHRtoEEEAEAAA-dsRNAresDepositorInsertDmPAN3-FLLWGYHRtoEEEAEAAA-dsRNAres (CG11486 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN3-FLLYHRtoEEEAAA-dsRNAres_Y
Plasmid#148084PurposeInsect Expression of DmPAN3-FLLYHRtoEEEAAA-dsRNAresDepositorInsertDmPAN3-FLLYHRtoEEEAAA-dsRNAres (CG11486 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN3-G711E-dsRNAres_Y
Plasmid#148085PurposeInsect Expression of DmPAN3-G711E-dsRNAresDepositorInsertDmPAN3-G711E-dsRNAres (CG11486 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN3-L766EV768E-dsRNAres_Y
Plasmid#148086PurposeInsect Expression of DmPAN3-L766EV768E-dsRNAresDepositorInsertDmPAN3-L766EV768E-dsRNAres (CG11486 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN3-W707AG711EV765R-dsRNAres_Y
Plasmid#148088PurposeInsect Expression of DmPAN3-W707AG711EV765R-dsRNAresDepositorInsertDmPAN3-W707AG711EV765R-dsRNAres (CG11486 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN3-W707E-dsRNAres_Y
Plasmid#148089PurposeInsect Expression of DmPAN3-W707E-dsRNAresDepositorInsertDmPAN3-W707E-dsRNAres (CG11486 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN2-L766EV758E-dsRNAres_Y
Plasmid#148047PurposeInsect Expression of DmPAN3-L766EV758E-dsRNAresDepositorInsertDmPAN3-L766EV758E-dsRNAres (CG11486 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-EGFP-DmPAN3-dsRNAres_Y
Plasmid#148050PurposeInsect Expression of DmPAN3-dsRNAresDepositorInsertDmPAN3-dsRNAres (CG11486 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only