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Plasmid#118908Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal HIS10, cleavable with 3C protease and C-terminal YBBR tagDepositorInsertNcoI-NotI-ccdB-AscI-YBBR-3C-HIS10-stop-HindIII cassette
UseTagsYBBR tag, HIS10, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCin4-3xFLAG-MKL1 D444-630
Plasmid#19840DepositorInsertMKL1 D444-630 (MRTFA Human)
UseTags3xFLAGExpressionMammalianMutationdeleted amino acids 444-630PromoterAvailable sinceMarch 16, 2009AvailabilityAcademic Institutions and Nonprofits only -
pNB695
Plasmid#60144PurposeDisintegrator plasmid encoding MET17 promoter driven green beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertGrLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNB697
Plasmid#60146PurposeDisintegrator plasmid encoding MET17 promoter driven red beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA).DepositorInsertRdLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0011
Plasmid#117530PurposeLevel0 Golden Gate part: CDS, Sp-xCas9 3.7 (no stop codon)DepositorInsertSp-xCas9 3.7 (no stop codon)
UseCRISPRTagsExpressionMutationA262T, R324L, S409I, E480K, E543D, M694I, E1219VPromoterAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5
Plasmid#52540PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pIS1 IMP-1 rc
Plasmid#21643DepositorInsertIGF-II mRNA-binding protein 1 (IGF2BP1 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterAvailable sinceJuly 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PARL-FLAG-CT S70A
Plasmid#13620DepositorUseTagsFLAGExpressionMammalianMutationchanged Serine 70 to AlaPromoterAvailable sinceJan. 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pBGO
Plasmid#62671PurposeDonor vector for mFTH1 knock-in via landing padDepositorInsertFTH1
UseCre/LoxTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5-mEKLF
Plasmid#67838PurposeExpression of murine EKLF (nucleotides 71-1215) driven by SV40 promoterDepositorInsertEKLF (Klf1 Mouse)
UseTagsnoneExpressionMammalianMutationPromoterSV40Available sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pICSL80037
Plasmid#68260PurposeLevel 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)DepositorInsertCoding sequence, neomycin phophotransferase II (Escherichia coli)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL11061
Plasmid#68255PurposeLevel 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleraceaDepositorInsertPromoter_AtU6-26 + sgRNA_BolC.GA4a-1
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pICSL11058
Plasmid#68254PurposeLevel 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barleyDepositorInsertPromoter_TaU6 + sgRNA_HvPM19_3
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL718
Plasmid#62826PurposeLim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1DepositorInsertsgfp
mCherry
UseSynthetic BiologyTagsipex leader and ompC leaderExpressionBacterialMutationPromoterpLlacO-1 and pLtetO-1Available sinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5
Plasmid#52542PurposeExpression of FRT5-flanked Neo; homologous recombination targeting vector cloningDepositorInsertsPhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
myc-actin-NES-1
Plasmid#60618PurposeEncodes MYC tagged beta-actin with a mutation in the NESDepositorInsertBeta-actin (ACTB Human)
UseTagsMYCExpressionBacterial and MammalianMutationI178A, L180APromoterAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGEX-4T3-ZO-2-1st-PDZ
Plasmid#27424DepositorInsertZonula Occludens 2 (TJP2 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceMarch 31, 2011AvailabilityAcademic Institutions and Nonprofits only