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Showing: 9801 - 9840 of 148587 results

  2. AICSDP-25:MAP1LC3B-mEGFP

    Plasmid
    #101783
    Purpose
    Homology arms and mEGFP-linker sequence for N-terminus tagging of human MAP1LC3B
    Depositor
    Allen Institute for Cell Science
    Insert
    MAP1LC3B Homology Arms with mEGFP-linker (MAP1LC3B Human)
    Use
    CRISPR; Donor template
    Tags
    mEGFP-linker
    Expression
    Mammalian
    Mutation
    homology arms contain point mutations to disrupt …
    Promoter
    Available since
    Oct. 31, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    101783-plasmid-map-sequence-id-197671

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  3. iCas

    Plasmid
    #84232
    Purpose
    Expression of SpCas9 with 4 ERT2 fusion protein and empty gRNA cassette. The activity of Cas9 can be switched on and off in human cells with 4-hydroxytamoxifen (4-HT)
    Depositor
    Meng How Tan
    Article
    Liu et al Nat Chem Biol. 2016 Sep 12. doi: 10.1038
    Insert
    Cas9
    Use
    Tags
    2A-OFP co-expression and ERT2-ERT2
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Nov. 28, 2016
    Availability
    Academic Institutions and Nonprofits only
    84232-plasmid-map-sequence-id-323254

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  13. pJYDN

    Plasmid
    #162450
    Purpose
    Yeast display plasmid intended for expression of N-terminal fusions with Aga2p yeast agglutinin and eUnaG2 bilirubin dependent green-yellow fluorescent protein for detection of expression.
    Depositor
    Gideon Schreiber
    Article
    Zahradník et al bioRxiv Preprint 2020
    Type
    Empty backbone
    Use
    Shuttle vector bacteria/yeast
    Tags
    Aga2p, HA, myc tags, and eUnaG2 fluorescent prote…
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    162450-plasmid-map-sequence-id-321442

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  17. p5E-EF1a/b-actin

    Plasmid
    #82583
    Purpose
    5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression
    Depositor
    Kryn Stankunas
    Article
    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi
    Insert
    EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
    Use
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    Dec. 21, 2016
    Availability
    Academic Institutions and Nonprofits only
    82583-plasmid-map-sequence-id-161169

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  21. pBS31-cCARLIN

    Plasmid
    #160928
    Purpose
    Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)
    Depositor
    Fernando Camargo
    Article
    Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.
    Insert
    CARLIN gRNAs and CAG-GFP-CARLIN array
    Use
    Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 promoter for gRNAs; CAG promoter for GFP
    Available since
    March 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    160928-plasmid-map-sequence-id-421629

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  23. IDG_TMC3_OE_1

    Plasmid
    #161629
    Purpose
    Overexpresses TMC3
    Depositor
    Mike McManus
    Insert
    TMC3 (TMC3 Human)
    Use
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    161629-plasmid-map-sequence-id-319361

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  30. pcDNA3.1-Letm1

    Plasmid
    #191962
    Purpose
    Expression of Letm1 (Leucine Zipper And EF-Hand Containing Transmembrane Protein 1).
    Depositor
    Luca Scorrano
    Insert
    Letm1 (LETM1 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    191962-plasmid-map-sequence-id-379658

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  35. pFX-mito-EGFP

    Plasmid
    #159096
    Purpose
    Express EGFP with mitochondria-targeting sequence in mammalian cells
    Depositor
    Yusuke Ohba
    Article
    Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183
    Insert
    mCherry with mitochondria-targeting sequence
    Use
    Tags
    the mitochondria-targeting sequence of the cytoch…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    159096-plasmid-map-sequence-id-354796

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  38. pMN-332

    Plasmid
    #195466
    Purpose
    Lentiviral Integratable Inducible synZiFTR ZF1 + Reporter
    Depositor
    Ahmad Khalil
    Article
    Li et al Science. 2022 Dec 16;378(6625):1227-1234
    Insert
    inverted[8X ZF1 BS - ybTATA - mCherry] - pSFFV - ABI1 - ZF1 - 2A - p65 - PYL1
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    195466-plasmid-map-sequence-id-384256

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  39. pcDNA3.1+(rInsp)-Ins-GLuc

    Plasmid
    #89928
    Purpose
    Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.
    Depositor
    Melanie Cobb
    Article
    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E
    Insert
    human insulin-Gaussia-Luciferase (INS Synthetic, Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    DNA sequence for Gaussia luciferase was humanized…
    Promoter
    rat Insulin II promoter
    Available since
    Sept. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    89928-plasmid-map-sequence-id-255702

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Showing: 9801 - 9840 of 148587 results