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pDONR221_SLC12A4
Plasmid#131932PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectorsDepositorInsertSLC12A4 (SLC12A4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA SF-HERC2 F2
Plasmid#55785PurposeExpresses N-terminal 3xFLAG/STREP tagged HERC2 (950-1750aa)DepositorInsert3xFLAG tagged HERC2 (950aa-1750aa) (HERC2 Human)
UseTags3xFLAG and STREPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-CSF1R-V5/HIS
Plasmid#201984Purposeexpression of human CSF1R receptor tyrosine kinase in mammalian cellsDepositorInsertCSF1R receptor tyrosine kinase (CSF1R Human)
UseTagsV5, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGEM-3z/601
Plasmid#26656DepositorInsert
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
myrAkt delta4-129
Plasmid#10841DepositorInsertAkt (AKT1 Human)
UseTagsHA and myrExpressionMammalianMutationDeletion of PH domain (aa4-129)PromoterSV40Available sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-hRNF216(457-784)
Plasmid#171922PurposeExpression of human RNF216 (Triad3A) codon optimized for E. coli and insect cells. Catalytic helix-RBR-helix construct suitable for activity assays.DepositorInsertE3 ubiquitin-protein ligase RNF216 (RNF216 Human)
UseTags3C protease cleavage site and 6x-His tagExpressionBacterialMutationcodon optimised for expression in E. coliPromoterT7Available sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDR111_Bgal no NLS
Plasmid#188397PurposeExpresses beta-galactosidase via Phyper-spank which is optimized for Bacillus subtilis. There is a secretion peptide (PhoD) that is fused to beta-galactosidase.DepositorInsertlacZ
UseTagsPhoD secretion peptideExpressionBacterialMutationPromoterPhyper spankAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL3Basic_ME.1/ApoEpromoter
Plasmid#51435PurposeThis plasmid is a luciferase-based reporter construct to measure the activity of the ApoE promoter fused to ME.1 enhancerDepositorUseLuciferaseTagsExpressionMammalianMutationME.1 enhancer is fused upstream of ApoE promoter …PromoterAvailable sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST14-Cys-SpyCatcher002
Plasmid#102829PurposeBacterial expression of Cysteine-modified SpyCatcher002 allowing reaction with thiol coupling reagentsDepositorInsertCys-SpyCatcher002
UseTagsGSSGS, His6, and TEV cleavage siteExpressionBacterialMutationCysteine residue in N-terminal regionPromoterT7Available sinceDec. 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pQC membrane TdTomato IX
Plasmid#37351DepositorInsertmembrane TdTomato
UseTagsPalmitoylation signal*ExpressionMutationPromoterAvailable sinceJuly 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTD103luxI_sfGFP
Plasmid#48885PurposePositive feedback plasmid from oscillatorDepositorInsertLuxI, sfGFP, and LuxR
UseSynthetic BiologyTagsssrA tag (AANDENYALAA)ExpressionBacterialMutationPromoterpLuxAvailable sinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBbB8c-GFP
Plasmid#35362DepositorInsertGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBADAvailable sinceMarch 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
FLAG-Foxo1-ADA 6KQ
Plasmid#17563DepositorInsertFoxo1 ADA 6KQ (Foxo1 Mouse)
UseTagsFlag and MycExpressionMammalianMutationK242, K245, K259, K262, K271 and K291 were replac…PromoterCMVAvailable sinceOct. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
Gi1-CASE
Plasmid#168120PurposeEncodes a BRET-based activity sensor for the heterotrimeric G protein Gi1. Composed of the subunits G alpha i1 (GNAI1) tagged with NanoLuciferase, G beta 1 (GNB1) and Venus-tagged G gamma 2 (GNG2).DepositorUseLuciferaseTagscpVenus on GNG2ExpressionMammalianMutationNLuc is inserted at A121/E122 within GNAI1PromoterAvailable sinceJuly 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-Hs4E-T_AF
Plasmid#148687PurposeMammalian Expression of Hs4E-TDepositorInsertHs4E-T (EIF4ENIF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hERalpha
Plasmid#101141PurposeMammalian expression of full-length human estrogen receptor-alphaDepositorInsertestrogen receptor-alpha (ESR1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceSept. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL2314
Plasmid#137054PurposeloxP (empty) expression cassette: c-terminal 6xHADepositorTypeEmpty backboneUseLeishmania floxed gene expressionTags6xHAExpressionMutationPromoterAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
modRNAc0-Cas9-2A-Puro
Plasmid#172855PurposeIVT template plasmid for making modified Cas9Puro RNADepositorInsertCas9-2A-Puro
UseIvt template plasmidTags2A-PuroExpressionMutationPromoterAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA HA FAK K454R
Plasmid#50501DepositorInsertprotein tyrosine kinase, mutated
UseTagsHAExpressionMammalianMutationK454RPromoterCMVAvailable sinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC19/N-Vc
Plasmid#183157PurposeBimolecular fluorescence complementation (BiFC) vector for transient expression of C-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vc.DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
myr-FLAG-AKT1-pcw107
Plasmid#64606Purposewhen used to produce lentivirus, express physiological levels of insertDepositorInsertAKT1 (AKT1 Human)
UseLentiviralTagsFLAG and myristoylatedExpressionMutationPromoterPGKAvailable sinceMay 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDx_mScarlet3-NCwt-SYFP2
Plasmid#189766PurposeDual expression vector for bacteria and mammalian cells producing mScarlet3-NCwt-SYFP2 fusion protein for FRET analysisDepositorInsertmScarlet3-NCwt-SYFP2
UseTags6xHisExpressionBacterial and MammalianMutationencodes the expected internal mutations relative …PromoterCMV & RhaAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPINS-USP21 (USP, aa 196-565)
Plasmid#61585PurposeExpresses human USP21 (USP domain) in E. coli.DepositorInsertUSP21 (Ubiquitin carboxyl-terminal hydrolase 21) (USP21 Human)
UseTagsHis6-SUMOExpressionBacterialMutationDeleted aa 1-195.PromoterT7Available sinceMarch 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMXs-puro-RFP-ATG9A
Plasmid#60609PurposeExpresses RFP-hATG9A in mammalian cellsDepositorInsertHomo sapiens autophagy related 9A (PPARG Human)
UseRetroviralTagsRFPExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
EC67842
Plasmid#209516PurposeCloning in complete guide expression cassettes, with Cas12a (D156R)DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
p426-SNR52p-gRNA.CAN1.Y-SUP4t
Plasmid#43803PurposeEncodes a gRNA that targets Can1.Y in yeast.DepositorArticleInsertCAN1.y gRNA (CAN1 Budding Yeast)
UseCRISPRTagsExpressionYeastMutationPromoterSNR52Available sinceMarch 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFastBac cloning vector with BioBrick PolyPromoter LIC Subcloning (438-A)
Plasmid#55218DepositorTypeEmpty backboneUseTagsExpressionInsectMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGWB444
Plasmid#74830PurposeGateway cloning compatible binary vector for C-terminal fusion with eCFP (CaMV35S promoter).DepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterCaMV35SAvailable sinceJuly 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSG28-J23151-AraC
Plasmid#102453PurposeExpresses AraC protein in E. coli. The expression is controlled by a constitutive promoter.DepositorInsertAraC (araC )
UseTagsExpressionBacterialMutationPromoterJ23151Available sinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
(133) pcDNA3.1-Nup62-Flag-EPEA
Plasmid#160744PurposeExpresses the nucleoporin Nup62 with C-terminal Flag and EPEA tags. Proteins of interest can be inserted by replacing Nup62 using Sgfl + Sgsl restriction enzymesDepositorInsertNup62-Flag-EPEA
UseTagsEPEA and FlagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 SARS-CoV-2 N
Plasmid#158079PurposeExpresses SARS-CoV-2 nucleocapsid protein in mammalian cellsDepositorInsertSARS-CoV-2 nucleocapsid (N )
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pscAAV-CBh-aSyn-WPRE3-enSV40pA
Plasmid#194244PurposeExpresses human alpha-Synuclein WTDepositorInsertaSynuclein(WT) (SNCA Human)
UseAAVTagsExpressionMutationPromoterCBhAvailable sinceJan. 17, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available sinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_V5-NEUROD1
Plasmid#215031PurposeMammalian expression of human NEUROD1DepositorInsertneuronal differentiation 1 (NEUROD1 Human)
UseTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIVT2
Plasmid#122140PurposeFor making cRNA appropriate for microinjectionDepositorTypeEmpty backboneUseFor in vitro transcriptionTagsExpressionMutationPromoterAvailable sinceMarch 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMV-Brainbow-2.1 R
Plasmid#18723DepositorInserthrGFPII-NLS ; EYFP ; tdimer2 ; M-mCerulean
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
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AKARdual
Plasmid#99869PurposeMeasure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrateDepositorInsertAKAR4
UseTagsLSSmOrange and mKate2ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-BFP
Plasmid#188776PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9-mTagBFP2
UseLentiviralTagsHA-2xNLS-mTagBFP2 and Zim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only
