-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 10841 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepECE
-
Backbone manufacturerWilliam Rutter
- Backbone size w/o insert (bp) 2924
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameAkt
-
Alt namemyr Akt deltaPH
-
SpeciesH. sapiens (human)
-
Insert Size (bp)1500
-
MutationDeletion of PH domain (aa4-129)
-
Entrez GeneAKT1 (a.k.a. AKT, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA)
- Promoter SV40
-
Tags
/ Fusion Proteins
- myr (N terminal on insert)
- HA (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer SV40pro-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Constitutively active Akt. 14 aa src myristoylation signal sequence fused to N terminus of Akt delta4-129.
Note: Addgene NGS results detected an 8 bp deletion in the 5' end of the SV40 promoter. Plasmid should function as described in the associated publication.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
myrAkt delta4-129 was a gift from Richard Roth (Addgene plasmid # 10841 ; http://n2t.net/addgene:10841 ; RRID:Addgene_10841) -
For your References section:
Akt, a pleckstrin homology domain containing kinase, is activated primarily by phosphorylation. Kohn AD, Takeuchi F, Roth RA. J Biol Chem. 1996 Sep 6. 271(36):21920-6. 10.1074/jbc.271.36.21920 PubMed 8702995