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Plasmid#52656PurposeExpresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.DepositorInsertTEM-1 β-Lactamase
UseTagsSpyCatcher and SpyTagExpressionBacterialMutationPromoterT7 promoterAvailable sinceMay 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Flag-RPA2
Plasmid#22893DepositorInsertRPA2 (RPA2 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceJan. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
pAB1
Plasmid#62547Purposebroad host range plasmid for IPTG-inducible expression of eGFP. Designed for imaging Pseudomonas aeruginosa activity in biofilms.DepositorInserteGFP
UseBroad host range plasmidTagsExpressionBacterialMutationPromoterpTrcAvailable sinceApril 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 blast tBID-flag
Plasmid#211533PurposeExpresses truncated BID upon doxycycline addition to trigger apoptosisDepositorInserttBID-flag (BID Human)
UseRetroviralTagsFLAGExpressionMammalianMutationTruncated (CASP8) form (p15)PromoterAvailable sinceJan. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSB1K3_pBen-sfGFP_J23101-mRFP
Plasmid#128126PurposeExpressing sfGFP under control of benzoate responsive promoter (pBen) and expressing mRFP under constitutive promoter J23101, and RBS B0032DepositorInsertsfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpBen (benzoate responsive promoter)Available sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pegfp-N1-TFEB-S3A,R4A
Plasmid#44446DepositorInsertTFEB (TFEB Human)
UseTagsGFPExpressionMammalianMutationChanged both Serine 3 and Arginine 4 to AlaninePromoterCMVAvailable sinceApril 26, 2013AvailabilityAcademic Institutions and Nonprofits only -
GfaABC1D-cyto-Ruby3-iATPSnFR1.0
Plasmid#102554Purposeexpresses cytoplasmic ATP sensor in astrocytes with red reference proteinDepositorInsertmRuby3-iATPSnFR1.0
UseAAVTagsExpressionMutationPromoterAvailable sinceJan. 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-U6-DR-SgRNA-DR-EF1a-mCherry-SV40
Plasmid#154002PurposeEmpty SgRNA expression system matching CasRxDepositorInsertSgRNA expression system
UseCRISPRTagsExpressionMutationPromoterU6, EF1aAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_25bpLinker
Plasmid#157788PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 25 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_25bp linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_193 NRL + TetO
Plasmid#157812PurposeEcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_tetO
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
Rc/CMV cyclin A
Plasmid#8959DepositorInsertcyclin A (CCNA2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pAAV Syn intron Psam4GlyR m3m4 HA
Plasmid#196041PurposeAAV for Chemogenetic plasmidsDepositorInsertPsam4GlyR m3m4 HA
UseAAVTagsHAExpressionMammalianMutationPromoterSynapsinAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPCK
Plasmid#133128Purposea recombinant plasmid, expressing pckA PEP-carboxykinase) geneDepositorInsertPEP-carboxykinase gene
UseTagsExpressionBacterialMutationPromoterPEP-carboxykinase promoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWM_12x601_45bpLinker
Plasmid#157791PurposeEcoRV digestion produces 12x601 chromatin assembly construct with 45 bp linker lengths in addition to carrier DNA that prevents overassembly of nucleosomesDepositorInsert12x601_45bp linker
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
HP138-neo
Plasmid#134247PurposeCas9 in a transposon array under a Tet inducible promoter. For making inducible Cas9 cell lines. Neo/G418 selectionDepositorInsertContains Cas9
UseCRISPRTagsExpressionMutationPromoterAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDAS12125_PEAR-GFP
Plasmid#177178PurposePEAR-GFP target plasmid with split EGFP and disrupted 5' splice siteDepositorInsertEGFP split with an intron between amino acids 95-96
UseCRISPRTagsExpressionMammalianMutationdisrupted 5' splice sitePromoterCMVAvailable sinceFeb. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
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GFP SMG1 WT
Plasmid#199571PurposeExpresses WT SMG1 fused to GFPDepositorInsertSMG1 WT (SMG1 Human)
UseTagsGFP fusion proteinExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSS9
Plasmid#71655PurposeContains 600 bp homology arms to SS9 for one-step markerless genome integration in E. coliDepositorInsertgfpuv
UseSynthetic BiologyTagsExpressionMutationPromoterT7A1Available sinceJan. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-CasRX-P2A-EGFP-BGH PolyA
Plasmid#154004PurposeExpress CasRxDepositorInsertCasRx
UseCRISPRTagsExpressionMutationPromoterCAGAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only