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PurposeExpress CasRx
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 154004 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonePB
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin and Kanamycin, 100 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCasRx
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Alt nameNLS-RfxCas13d-NLS
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SpeciesSynthetic
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Insert Size (bp)5753
- Promoter CAG
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
INSERT: CasRx & EGFP driven by CAG promoter.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PB-CAG-CasRX-P2A-EGFP-BGH PolyA was a gift from Hui Yang (Addgene plasmid # 154004 ; http://n2t.net/addgene:154004 ; RRID:Addgene_154004) -
For your References section:
Modulation of metabolic functions through Cas13d-mediated gene knockdown in liver. He B, Peng W, Huang J, Zhang H, Zhou Y, Yang X, Liu J, Li Z, Xu C, Xue M, Yang H, Huang P. Protein Cell. 2020 Mar 18. pii: 10.1007/s13238-020-00700-2. doi: 10.1007/s13238-020-00700-2. 10.1007/s13238-020-00700-2 PubMed 32185621