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Plasmid#204747PurposeExpression of Cas9 and human H2AX with the K5R mutationDepositorInsertH2AX K5R (H2AX Human)
UseTagsExpressionMammalianMutationH2AX with the K5R mutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_S139D-GS3
Plasmid#204748PurposeExpression of Cas9 and human H2AX with the S139D mutationDepositorInsertH2AX S139D (H2AX Human)
UseTagsExpressionMammalianMutationH2AX with the S139D mutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_S139A-GS3
Plasmid#204749PurposeExpression of Cas9 and human H2AX with the S139A mutationDepositorInsertH2AX S139A (H2AX Human)
UseTagsExpressionMammalianMutationH2AX with the S139A mutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134L-GS3
Plasmid#204750PurposeExpression of Cas9 and human H2AX with the K134L mutationDepositorInsertH2AX K134L (H2AX Human)
UseTagsExpressionMammalianMutationH2AX with the K134L mutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134M-GS3
Plasmid#204751PurposeExpression of Cas9 and human H2AX with the K134M mutationDepositorInsertH2AX K134M (H2AX Human)
UseTagsExpressionMammalianMutationH2AX with the K134M mutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCPH2A.X_K134A-GS3
Plasmid#204752PurposeExpression of Cas9 and human H2AX with the K134A mutationDepositorInsertH2AX K134A (H2AX Human)
UseTagsExpressionMammalianMutationH2AX with the K134A mutationPromoterAvailable sinceJune 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
VIPR2-DuET
Plasmid#213387PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorInsertVIPR2 (VIPR2 Human)
UseTags1D4 and FLAGExpressionMammalianMutationPromoterCMV, T7Available sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGMC00033 (aka pMC0224)
Plasmid#210750PurposeGuide RNA targeting SERPINB3DepositorInsertSERBPINB3 guide RNA
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
UAS-dsarm(E,K450R)
Plasmid#187881PurposeGal4/UAS expression of dSarm(isoE,K450R)DepositorInsertdSarm isoform E (Sarm )
UseTagsExpressionMutationK450RPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE_NFL linker-3xFLAG KI
Plasmid#182679PurposeExpression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker-3xFLAG. Can be used for C-terminal tagging of endogenous NFL with a linker-3xFLAG tag.DepositorInsertNefl-targeting gRNA and linker-3xFLAG donor sequence (Nefl Mouse)
UseTagsExpressionMammalianMutationPromoterU6 and chicken beta-actin promoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pORANGE_NFL linker(A6TAG)-3xFLAG KI
Plasmid#182680PurposeExpression of spCas9, gRNA targeting the end of mouse Nefl gene and donor linker(A6TAG)-3xFLAG. Can be used for amber codon suppression and click chemistry labeling of endogenous NFL.DepositorInsertNefl-targeting gRNA and linker(A6TAG)-3xFLAG donor sequence (Nefl Mouse)
UseTagsExpressionMammalianMutationPromoterU6 and chicken beta-actin promoterAvailable sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL6-Citrine:NOSt
Plasmid#175568PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-6DepositorInsertGOXL6
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX330 NLC-FKBP-Cas9
Plasmid#162726PurposeMammalian expressionDepositorInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330 C-FKBP-Cas9
Plasmid#162729PurposeMammalian expressionDepositorInsertFKBP F36V
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBhAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL5-Citrine:NOSt
Plasmid#175567PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-5DepositorInsertGOXL5
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL4-Citrine:NOSt
Plasmid#175566PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-4DepositorInsertGOXL4
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL3-Citrine:NOSt
Plasmid#175565PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-3DepositorInsertGOXL3
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGreenII 0229/ProRUBY:GOXL2-Citrine:NOSt
Plasmid#175564PurposepSOUP helper plasmid needed for Agrobacterium-mediated plant transformation - Expression of GOXL genes tagged with Citrine under RUBY promoter for transgene complementation of ruby-2DepositorInsertGOXL2
UseTagsCitrineExpressionPlantMutationPromoterRUBYAvailable sinceOct. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA™5/FRT/CMV_promoter-ciCas9-E2A-mRFP
Plasmid#164132PurposeExpresses ciCas9 and mRFP in mammalian cells (an insertion vector to generate ciCas9-knockin cell lines)DepositorInsertciCas9
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGB3 alpha2 P35S:RDF:T35S (GB1508)
Plasmid#160613PurposeTU for the constitutive expression of Streptomyces phage PhiC31-encoded recombination directionality factor (RDF) (PhiC31 gp3)DepositorInsertRDF
UseSynthetic BiologyTagsExpressionPlantMutationBsaI and BsmBI sites removedPromoter35SAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only