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Plasmid#222431PurposeFRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.DepositorInsertMitochondrial outer membrane delta-selective C kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
CMV-pDisplay-SNAP[C145A]
Plasmid#223496PurposeExpression of dead-mutant SNAP-tags to outer surface in mammalian cellsDepositorInsertdead-mutant SNAP-tag
UseTagsExpressionMammalianMutationSNAP-tag C145APromoterAvailable sinceAug. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-HGM (optimized)
Plasmid#220988PurposeAdeno-associated viral vector to express HaloTag-GFP-mito fusion, a cytosolic-localized Halotag-GFP fusion constructDepositorInsertHaloTag-GFP-mito
UseAAVTagsExpressionMutationPromoterAvailable sinceAug. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4-R115A/R119A
Plasmid#80696PurposeExpresses Csy4 with R115A and R119A mutations from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4-R115A/R119A
UseAAVTagsExpressionMammalianMutationArg115 and Arg119 both mutated to AlaPromoterCBAAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CHIKV-Envelope protein
Plasmid#215700PurposeProduces the Chikungunya virus envelope protein E3-E2-6K-E1DepositorInsertCHIKV E3-E2-6K-E1
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-eGFP-NES-NFATC2IP
Plasmid#221446PurposeMammalian expression vector of eGFP tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminusDepositorInsertNFATC2IP (NFATC2IP Human)
UseTagsNuclear Export Signal (NES) and eGFPExpressionMammalianMutationNES (NLVDLQKKLEELELDEQQ) inserted, 1st Methionine…PromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-3XFLAG-NES-NFATC2IP
Plasmid#221444PurposeMammalian expression vector of triple Flag tagged NFATC2IP ORF with nuclear export signal (NES) at its N-terminusDepositorInsertNFATC2IP (NFATC2IP Human)
UseTags3XFlag and Nuclear Export Signal (NES)ExpressionMammalianMutationNES (NLVDLQKKLEELELDEQQ)inserted, 1st Methionine …PromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEN-X-mPGK-NeoR-SV40 polyA-Z
Plasmid#218909Purposegolden gate cloningDepositorInsertmPGK-Neomycine-SV40 polyA
UseTagsExpressionMammalianMutation395T>CPromoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRER-PhoCl-Insert
Plasmid#219675PurposeCaging at lumenal N-ter of POI: TMEM-PhoCl-MCS in pTREtight2DepositorInsertRER-PhoCl
UseTagsPhoClExpressionMammalianMutationPromoterTREAvailable sinceJune 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pEN-X-mPGK-PuroR-SV40 polyA-Z
Plasmid#218908Purposegolden gate cloningDepositorInsertmPGK-PuroR-SV40 polyA
UseTagsExpressionMammalianMutation297T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-BlastR-Z
Plasmid#218907Purposegolden gate cloningDepositorInserthPGK-BlastR
UseTagsExpressionMammalianMutation246T>A and 398T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-NeoR-Z
Plasmid#218906Purposegolden gate cloningDepositorInserthPGK-NeoR
UseTagsExpressionMammalianMutation246T>A and 398T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-hPGK-HygroR-Z
Plasmid#218905Purposegolden gate cloningDepositorInserthPGK-HygroR
UseTagsExpressionMammalianMutation246T>A and 398T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEN-X-mPGK-BlastR-SV40 polyA-Z
Plasmid#218903Purposegolden gate cloningDepositorInsertmPGK-BlastR-SV40 polyA
UseTagsExpressionMammalianMutation2468T>CPromoterAvailable sinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxEXPr6
Plasmid#218290PurposeIntroduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3DepositorInserthph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJQR3
Plasmid#218289PurposeIntroduction of a Zif268-hER-VP16 expression cassette and a LmrA-Mig1C expression cassette, use together with pToxEXPR6DepositorInsertHO(-253,-1)-pADH1>LmrA>MIG1(aa123-504)>tMIG1-tYGL036WUseTagsExpressionYeastMutationPromoterAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1315
Plasmid#217957PurposeExpression of human H3.1 carrying a triple point mutation to disrupt folding with histone H4. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFPExpressionMammalianMutationF84A, L92A, Y99APromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCL1314
Plasmid#217956PurposeExpression of human H3.1 carrying a triple glycine insertion to disrupt folding with H4. Expression in mammalian cells driven by the CMV promoter.DepositorInsertHistone H3.1 (H3C1 Human)
UseTagseGFPExpressionMammalianMutationGGG insertion between A95 and C96PromoterCMVAvailable sinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV/CMV-SCLM
Plasmid#216758PurposeAAV2 transfer vector with the CMV promoter for ubiquitous expression of SuperClomeleonDepositorInsertSuperClomeleon followed by WPRE and human growth hormone polyA terminator
UseAAVTagsExpressionMammalianMutationS30R and 11 AA deletion in the Cerulean CFP moiet…PromoterCMV (cytomegalovirus)Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28_human-pro-IL-18_E192K_D193K
Plasmid#214322PurposeProtein overexpression in bacteriaDepositorInsertIL18 (IL18 Human)
UseTagsHis-TEVExpressionBacterialMutationE192K_D193KPromoterT7Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only