Showing: 4881 - 4900 of 12974 results
-
Plasmid#47872PurposeFLAG tagged NmCas9 with no NLS.DepositorInsertNmCas9
UseCRISPRTagsFLAGExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
SP6-hCas9-Ce-mRNA
Plasmid#47911PurposeIn vitro transcription of a mRNA encoding humanized Cas9 nuclease, with 3' UTRs suitable for germline expression in C. elegans, for doing CRISPR-Cas by RNA injection. Uses SP6 RNA polymerase.DepositorInserthCas9 with C. elegans 5' and 3' UTRs
UseCRISPRTagsExpressionMutationPromoterSP6AvailabilityAcademic Institutions and Nonprofits only -
SP6-sgRNA-scaffold
Plasmid#47912PurposeScaffold into which clone a 20 bp targeting sequence to generate a plasmid for in vitro transcription of an sgRNA using SP6 RNA polymerase for use in CRISPR-Cas by RNA injectionDepositorTypeEmpty backboneUseCRISPRTagssgRNA 3' endExpressionWormMutationPromoterSP6AvailabilityAcademic Institutions and Nonprofits only -
pCS2-nCas9n
Plasmid#47929Purposeexpression of an optimized Cas9 for genome-editing in zebrafishDepositorInsertnls-zcas9-nls
UseCRISPRTagsExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pT7goldRNA
Plasmid#47930PurposeIn vitro transcription of golden gRNADepositorInsertgolden gRNA (slc24a5 Zebrafish)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pT7mitfagRNA
Plasmid#47931Purposein vitro transcription of mitfa gRNADepositorInsertmitfa gRNA (mitfa Zebrafish)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pT7ddx19gRNA
Plasmid#47932Purposein vitro transcription of ddx19 gRNADepositorInsertddx19 gRNA (ddx19 Zebrafish)
UseCRISPRTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pIK86
Plasmid#47933PurposeC. elegans codon-optimized Cas9 with 2xSV40 NLS sequences. It has a T7 promoter for in vitro transcription, germline-compatible 5'UTR, tbb-2 3'UTR and a polyA sequence.DepositorInsertCas9
UseCRISPR and UnspecifiedTagsExpressionMutationPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pMB60
Plasmid#47941PurposeCan be used to generate synthetic guide in vitro from T7 promotorDepositorInsertsynthetic guide RNA
UseCRISPRTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMB70
Plasmid#47943PurposeCan be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequencesDepositorInsertguide RNA
UseCRISPRTagsExpressionWormMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pMB62
Plasmid#47944PurposeExpresses C. elegans optimized Cas9::mEGFP from the eef-1A.1 (eft-3) promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, egl-13 NLS, and mEGFPExpressionWormMutationPromotereef-1A.1 (eft-3)AvailabilityAcademic Institutions and Nonprofits only -
pMB63
Plasmid#47945PurposeExpresses C. elegans optimized Cas9 from the eef-1A.1 (eft-3) promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, and egl-13 NLSExpressionWormMutationPromotereef-1A.1 (eft-3)AvailabilityAcademic Institutions and Nonprofits only -
pMB66
Plasmid#47946PurposeExpresses C. elegans optimized Cas9::mEGFP from the hsp-16.48 promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, egl-13 NLS, and mEGFPExpressionWormMutationPromoterhsp-16.48AvailabilityAcademic Institutions and Nonprofits only -
pMB67
Plasmid#47947PurposeExpresses C. elegans optimized Cas9 from the hsp-16.48 promoterDepositorInsertCas9
UseCRISPRTags3xFLAG, SV40 NLS, and egl-13 NLSExpressionWormMutationPromoterhsp-16.48AvailabilityAcademic Institutions and Nonprofits only -
3xFLAG-dCas9/pCMV-7.1
Plasmid#47948PurposeExpresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.DepositorInsert3xFLAG-dCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationhuman codon-optimized, D10A + H840A (catalyticall…PromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9 (PX165)
Plasmid#48137PurposeHuman codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)DepositorInserthSpCas9
UseCRISPRTags3XFLAGExpressionMammalianMutationPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP (PX458)
Plasmid#48138PurposeCas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorHas ServiceCloning Grade DNAInserthSpCas9
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459)
Plasmid#48139PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.DepositorInserthSpCas9-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-GFP (PX461)
Plasmid#48140PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorInserthSpCas9n
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462)
Plasmid#48141PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62987DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailabilityAcademic Institutions and Nonprofits only
Showing: 4881 - 4900 of 12974 results