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PurposeCan be used to express a CRISPR guide RNA in C. elegans from U6 regulatory sequences
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 47943 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepMK
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Backbone manufacturerGeneart
- Total vector size (bp) 3655
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Vector typeWorm Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameguide RNA
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Alt namesgRNA
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SpeciesSynthetic
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Insert Size (bp)80
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For more information on Boxem Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/boxem/
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMB70 was a gift from Mike Boxem (Addgene plasmid # 47943 ; http://n2t.net/addgene:47943 ; RRID:Addgene_47943) -
For your References section:
CRISPR/Cas9-Targeted Mutagenesis in Caenorhabditis elegans. Waaijers S, Portegijs V, Kerver J, Lemmens BB, Tijsterman M, van den Heuvel S, Boxem M. Genetics. 2013 Aug 26. 10.1534/genetics.113.156299 PubMed 23979586