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pSpCas9(BB)-2A-GFP-G3BP1(2)

Plasmid

#136059

Purpose

G3BP1 gRNA (#2) inserted into the pSpCas9(BB)-2A-GFP plasmid (GTATTACACACTGCTGAACC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-HPGD-FLAGC

Plasmid

#161915

Purpose

Expresses human HPGD with a C-terminal FLAG-GFP tag. Confers G418 resistance.

Depositor

Sébastien Britton

Article

Demange et al Elife. 2022 May 10;11. pii: 73913. doi:

Insert

HPGD (HPGD Human)

Use

Tags

FLAG-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pENeGFP PCNAL2 pc653

Plasmid

#167564

Purpose

The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.

Depositor

Cristina Cardoso

Article

Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.

Insert

Proliferating Cell Nuclear Antigen (PCNA Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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EGFP-Septin 11 isoform II

Plasmid

#120317

Purpose

Septin 11 isoform II mammalian expression (EGFP-tag at the N-terminus)

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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ABCG1_pcDNA6.2/EmGFP-Bsd

Plasmid

#176944

Purpose

Mammalian expression vector encoding ABCG1 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

ABCG1 (ABCG1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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5K-tails-->R GLUT1-GFP

Plasmid

#200099

Purpose

human GLUT1 with K-->R mutation of 5 lysines on the N- and C-terminal tails with a C-terminal tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 451, 456,…

Promoter

CMV

Available since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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1K245 GLUT1-GFP

Plasmid

#200097

Purpose

human GLUT1 with a single cytosolic lysine (K245) and C-terminal GFP tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

K-->R mutations of amino acids 6, 7, 225, 229,…

Promoter

CMV

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJH625_pJH_LldR_PlldR-sfGFP.0TAG

Plasmid

#163724

Purpose

Lactate-inducible expression of green fluorescent protein encoding zero amber codons

Depositor

Matthew Bennett

Article

Ho et al Nucleic Acids Res. 2021 Mar 18;49(5):e25

Insert

lldr gfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV:GFP(FRT)DsRed

Plasmid

#31372

Depositor

Gerhart Ryffel

Article

Werdien et al Nucleic Acids Res. 2001 Jun 1. 29(11):E5

Insert

GFP(FRT)DsRed

Use

Flp/frt

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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pg-proPHD2-PHD2-EGFP

Plasmid

#87210

Purpose

PHD2-EGFP expressed under the control of the PHD2 promoter

Depositor

Violaine Sée

Article

Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

Insert

PHD2 (EGLN1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

H313N

Promoter

PHD2

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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AICSDP-41: ATP2A2-mEGFP

Plasmid

#114405

Purpose

Homology arms and mEGFP-linker sequence for N-terminus tagging of human ATP2A2

Depositor

Allen Institute for Cell Science

Insert

ATP2A2 Homology Arms with mEGFP-linker (ATP2A2 Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mammalian

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

Sept. 4, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pRSETB-roGFP1-R12

Plasmid

#83311

Purpose

Inducible expression of redox sensitive GFP in bacteria

Depositor

S. James Remington

Article

Cannon et al Protein Sci. 2006 Jan;15(1):45-57. Epub

Insert

roGFP1-R12

Use

Tags

6xHIS

Expression

Bacterial

Mutation

C48S/Q80R/S147C/Q204C/N149K/F223R/S202K

Promoter

T7

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only
FCK-Mac-GFP

Plasmid

#22223

Purpose

Lentiviral expression of Mac-GFP for optogenetic inhibition

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

Mac

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

NO

Promoter

CaMKII

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta2

Plasmid

#101290

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

1-727 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-ninein C-ter

Plasmid

#73523

Purpose

Encodes the C-terminus of mouse ninein (residues 1874-2113) with an EGFP tag at its N-terminus

Depositor

Michel Bornens

Article

Delgehyr et al J Cell Sci. 2005 Apr 15;118(Pt 8):1565-7

Insert

ninein C-ter (residues 1874-2113) (Nin Mouse)

Use

Tags

EGFP tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 29, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA5-GFP-PKHD1

Plasmid

#108408

Purpose

Can be used to generate stable Flp-In cells with tetracycline-inducible human polyduction/fibrocystin expression.

Depositor

Gregory Germino

Article

Kaimori et al Hum Mol Genet. 2007 Apr 15. 16(8):942-56

Insert

PKHD1 (PKHD1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pATZ-BFP-HA-GFP11

Plasmid

#177721

Purpose

ER protein alpha-1-antitrypsin containing E342K mutation (ATZ mutant) fused to blue fluorescence protein (BFP) tag protein, an HA tag and the eleventh β-strand of GFP

Depositor

Erik Böttger

Article

Cortes Sanchon et al Commun Biol. 2021 Dec 2;4(1):1350. doi:

Insert

alpha-1-antitrypsin (SERPINA1 Human)

Use

Tags

GFP11, HA, and TagBFP

Expression

Bacterial and Mammalian

Mutation

E342K (ATZ mutant)

Promoter

Available since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pBoxCDGC-KMet-EGFP

Plasmid

#140272

Purpose

L7Ae-dependent translational repression

Depositor

Hirohide Saito

Article

Saito et al Nat Chem Biol. 2010 Jan;6(1):71-8. doi:

Insert

BoxCD

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-p97-E578Q

Plasmid

#85672

Purpose

Expression of human p97 E578Q mutant with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

p97 (Vcp Rat)

Use

Tags

GFP

Expression

Mammalian

Mutation

E578Q

Promoter

CMV

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmFMR1_B

Plasmid

#145920

Purpose

Insect Expression of DmFMR1

Depositor

Elisa Izaurralde

Insert

DmFMR1 (Fmr1 Fly)

Use

Tags

Expression

Insect

Mutation

Two amino acid mutations (T214I and N501S) and th…

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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