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Plasmid#222015PurposeExpress human PHOSPHO1 protein (C-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cellDepositorUseTagsTEV cleavable site and Twin-Strep-tagExpressionMammalianMutationstop codon (TGA) is deletedPromoterchicken β-actin promoterAvailable sinceJan. 8, 2025AvailabilityAcademic Institutions and Nonprofits only
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pLentiCRISPRRv2_sgTom
Plasmid#218346PurposeCRISPR KO plasmid for tdTomato in human cell linesDepositorInsertInserted sgRNA targetting tdTomato
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFastBac SOX 318-320A
Plasmid#192060PurposeExpression vector for SOX (ORF37) 318-320ADepositorInsertSOX (ORF37)
UseTagsExpressionInsectMutation318-320APromoterPolyhedrinAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
SOX D221A/E244A/K246A pACEmam1
Plasmid#192050PurposeExpresses a 3X catalytic mutant of SOX (ORF37)DepositorInsertSOX (3X catalytic mutant)
UseUnspecifiedTagsExpressionMutation3X catalytic mutant D221A/E244A/K246APromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACE SOX (ORF37) K246A
Plasmid#192051PurposeExpresses a catalytic mutant of SOX (K246A)DepositorInsertSOX (K246A)
UseUnspecifiedTagsExpressionMutationCatalytic mutant K246APromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACE SOX (ORF37) K250A
Plasmid#192052PurposeExpresses a DNA binding mutant K250A (ORF37)DepositorInsertSOX (K250A)
UseUnspecifiedTagsExpressionMutationDNA binding mutant K250APromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACE SOX (ORF37) 318-320A
Plasmid#192054PurposeExpresses a mutant of SOX 318-320ADepositorInsertSOX (318-320A)
UseUnspecifiedTagsExpressionMutationSOX 318-320APromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMDC Renilla Luc SRE
Plasmid#191657PurposeExpressed Renilla Luciferase with SOX resistance elementDepositorInsertRenilla Luciferase SRE
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
mRFP-LAP2β
Plasmid#228029Purposelentiviral construct for mRFP-LAP2β expressionDepositorInsertLAP2b (Tmpo Rat)
UseLentiviralTagsmRFPExpressionMammalianMutationPromoterAvailable sinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2705-CMV-Nme2Smu-ABE8e-i8-L10-D56R
Plasmid#228726PurposeExpresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2Smu nCas9 (D16A)-i8-L10-D56R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D16A D56RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2704-CMV-Nme2Smu-ABE8e-i8-L10-E932R
Plasmid#228725PurposeExpresses domain inlaid Nme2Smu nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2Smu nCas9 (D16A)-i8-L10-E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2708-CMV-Nme2Smu-ABE8e-i1-L10-E932R
Plasmid#228729PurposeExpresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2Smu nCas9 (D16A)-i1-L10-E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2706-CMV-Nme2-ABE8e-i8-L10-E932R
Plasmid#228727PurposeExpresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and E932R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2 nCas9 (D16A)-i8-L10-E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2703-CMV-iNme2Smu-ABE8e-nt
Plasmid#228724PurposeExpresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cellsDepositorInsertN-terminal ABE8e with iNme2Smu nCas9 (D16A)
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2707-CMV-Nme2-ABE8e-i8-L10-D56R
Plasmid#228728PurposeExpresses domain inlaid Nme2 nCas9 (D16A)-i8-ABE8e base editor with linker-10 and D56R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2 nCas9 (D16A)-i8-L10-D56R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A D56RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2709-CMV-Nme2Smu-ABE8e-i1-L10-D56R
Plasmid#228730PurposeExpresses domain inlaid Nme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2Smu nCas9 (D16A)-i1-L10-D56R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D16A D56RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R
Plasmid#228732PurposeExpresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2 nCas9 (D16A)-i1-L10-D56R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A D56RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10
Plasmid#228735PurposeExpresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cellsDepositorInsertDomain inlaid iNme2Smu nCas9 (D16A)-i1-L10
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R
Plasmid#228731PurposeExpresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2713-CMV-iNme2-ABE8e-i1-L10
Plasmid#228734PurposeExpresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cellsDepositorInsertDomain inlaid iNme2 nCas9 (D16A)-i1-L10
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A D844G E868K K870R D873A D911G K929…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only