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Plasmid#78743PurposeHuman expression plasmid for AsCpf1-NLS-3xHADepositorInsertmammalian codon-optimized Cpf1-NLS-3xHA
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationPromoterCAGAvailable sinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-ATXN1-2Q
Plasmid#33239DepositorInsertAtaxin-1 (ATXN1 Human)
UseTagsGFPExpressionMammalianMutationinsert contains 2Q (2 glutamines) and there isno …PromoterAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTRIPZ shTREX1
Plasmid#127700PurposeDoxycyclin inducible shRNA knockdown of both human and mouse TREX1 geneDepositorInsertshTREX1 (Trex1 Mouse, Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn5-150
Plasmid#118532PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertTagBFP2
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-MFRN2
Plasmid#50546PurposeExpression of MFRN2 gene in mammalian cellsDepositorInsertMrfn2 (SLC25A28 Human)
UseTagsV5ExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV gfaABC1D NES-jRCaMP1a
Plasmid#171120PurposeAstrocyte AAV-mediated expression of jRCaMP1a, a red fluorescent calcium sensor protein.DepositorInsertjRCaMP1a
UseAAVTagsHis Tag, T7 Tag, XpressTagExpressionMammalianMutationPromotergfaABC1DAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV-pMecp2-SaCas9-pU6-sgRNA
Plasmid#159173PurposeVector A encodes pAAV-pMecp2-SaCas9-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of SaCas9-sgRNA in neuronsDepositorInsertSaCas9
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterpMecp2Available sinceNov. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pActin-Electra2
Plasmid#184942Purposebeta-actin labelling with blue fluorescent protein Electra2DepositorInsertElectra2-beta-actin
UseTagsElectra2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMRE-Tn7-155
Plasmid#118569PurposeminiTn7 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmScarlet-I
UseMinitn7 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceOct. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
BPK2139
Plasmid#65776PurposeHuman expression vector for S aureus Cas9: CAG-humanSaCas9-NLS-3xFLAGDepositorInsertmammalian codon-optimized Staphylococcus aureus Cas9-NLS-3xFlag
UseCRISPRTagsNLS-3xFLAGExpressionMammalianMutationPromoterCAGAvailable sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Csy4-PE2
Plasmid#172667PurposeEnhanced prime editing system in mammalian cells. This plasmid is used by PE3 and PE3bDepositorInsertCsy4-PE2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
LZRS-IresGFP
Plasmid#21961DepositorInsertIres GFP
UseRetroviralTagsExpressionMammalianMutationGACAGATGACTATGCAGAGATPromoterAvailable sinceSept. 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
pUB_smHA_KDM5B_MS2
Plasmid#81085PurposeTranslation reporter: Encodes KDM5B (1544 aa) fused to smHA (Spaghetti Monster HA: 10 copies of an epitope tag HA).DepositorInsertsmHA_KDM5B_24xMS2
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-IRAlus-Lin28
Plasmid#92347PurposePlasmid for expression of GFP gene with a Lin28 gene derived IR Alu element containing 3'UTR.DepositorInsertLin28 gene derived IR Alu element containing 3'UTR (LIN28A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJune 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJCC_076 AsCas12a R1226A
Plasmid#171669PurposeFor bacterial expression of AsCas12a R1226A (RuvC-inhibiting mutation) with an N-terminal His-MBP tagDepositorInsertAsCas12a R1226A
UseTags10X His, TEV protease cleavage site, and maltose-…ExpressionBacterialMutationchanged arginine 1226 to alaninePromoterAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
GFP-rab9 DN
Plasmid#12664DepositorInsertRAB9 (Homo sapiens) (RAB9A Human)
UseTagsGFP and HAExpressionMammalianMutationchanged serine 21 to asparaginePromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only -
EGFP-Ki67-mCherry
Plasmid#183748PurposeExpresses EGFP-Ki67-mCherryDepositorInsertMKI67 (MKI67 Human)
UseTagsKi-67 with N-terminal EGFP tag and Ki-67 with C-…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro SHP2 E76A
Plasmid#8331DepositorInsertSHP2 (PTPN11 Human)
UseRetroviralTagsExpressionMammalianMutationE76A (activated mutant)PromoterAvailable sinceJune 17, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT/TO Plk4 ∆24-mCherry
Plasmid#80271Purposemammalian expression plasmid for Plk4 with multi-phospho degron deletedDepositorInsertPlk4 (PLK4 Human)
UseTagsmCherryExpressionMammalianMutationdeletion of multi-phospho degron (amino acids 282…PromoterCMVAvailable sinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT PUR mGSK3AWT
Plasmid#35159DepositorInsertGSK-3α (Gsk3a Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMay 18, 2012AvailabilityIndustry, Academic Institutions, and Nonprofits