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pBT307_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PhiC31o-pA-FRT5

Plasmid

#52540

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

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pIS1 IMP-1 rc

Plasmid

#21643

Depositor

David Bartel

Article

Mayr et al Cell. 2009 Aug 21. 138(4):673-84.

Insert

IGF-II mRNA-binding protein 1 (IGF2BP1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

July 28, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT S70A

Plasmid

#13620

Depositor

Luca Pellegrini

Article

Jeyaraju et al Proc Natl Acad Sci U S A. 2006 Dec 5. 10

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

changed Serine 70 to Ala

Promoter

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pKG427

Plasmid

#69754

Purpose

Retroviral expression of DSN1

Depositor

Iain Cheeseman

Article

Gascoigne et al J Cell Biol. 2013 Apr 1;201(1):23-32. do

Insert

DSN1 (DSN1 Human)

Use

Retroviral

Tags

eGFP-TEV-S

Expression

Mammalian

Mutation

all K to R mutations

Promoter

Available since

Nov. 5, 2015

Availability

Academic Institutions and Nonprofits only
JW616

Plasmid

#64805

Purpose

CUC2 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

CUC2 (CUC2 Mustard Weed)

Use

Tags

GAL4 Activation Domain, HA, and SV40 NLS

Expression

Mutation

Promoter

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pBGO

Plasmid

#62671

Purpose

Donor vector for mFTH1 knock-in via landing pad

Depositor

Masato Ohtsuka

Article

Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

Insert

FTH1

Use

Cre/Lox

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2015

Availability

Academic Institutions and Nonprofits only

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pSG5-mEKLF

Plasmid

#67838

Purpose

Expression of murine EKLF (nucleotides 71-1215) driven by SV40 promoter

Depositor

James Bieker

Article

Miller et al Mol Cell Biol. 1993 May;13(5):2776-86.

Insert

EKLF (Klf1 Mouse)

Use

Tags

none

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
JW630

Plasmid

#64807

Purpose

TCP4 expression in yeast

Depositor

Detlef Weigel

Article

Rubio-Somoza et al Curr Biol. 2014 Nov 17;24(22):2714-9. do

Insert

TCP4 (TCP4 Mustard Weed)

Use

Tags

GAL4 Activation domain and HA

Expression

Mutation

Promoter

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL80037

Plasmid

#68260

Purpose

Level 0 Golden Gate Part: Coding sequence, neomycin phophotransferase II (Escherichia coli)

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Coding sequence, neomycin phophotransferase II (Escherichia coli)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL11061

Plasmid

#68255

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting BolC.GA4a-1 in Brassica oleracea

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_AtU6-26 + sgRNA_BolC.GA4a-1

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pICSL11058

Plasmid

#68254

Purpose

Level 1 Golden Gate Cassette: sgRNA cassette targeting PM19_3 in barley

Depositor

Nicola Patron

Article

Lawrenson et al Genome Biol. 2015 Nov 30;16(1):258. doi:

Insert

Promoter_TaU6 + sgRNA_HvPM19_3

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pHL718

Plasmid

#62826

Purpose

Lim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1

Depositor

Han Lim

Article

Sagawa et al Nucleic Acids Res. 2015 Aug 10. pii: gkv

Inserts

gfp
mCherry

Use

Synthetic Biology

Tags

ipex leader and ompC leader

Expression

Bacterial

Mutation

Promoter

pLlacO-1 and pLtetO-1

Available since

Aug. 14, 2015

Availability

Academic Institutions and Nonprofits only
pBT309a_pBS-U-attPx3-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52542

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo
PhiC31o integrase

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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myc-actin-NES-1

Plasmid

#60618

Purpose

Encodes MYC tagged beta-actin with a mutation in the NES

Depositor

Primal de Lanerolle

Article

Hofmann et al J Cell Biol. 2009 Jul 27;186(2):193-200.

Insert

Beta-actin (ACTB Human)

Use

Tags

MYC

Expression

Bacterial and Mammalian

Mutation

I178A, L180A

Promoter

Available since

Nov. 21, 2014

Availability

Academic Institutions and Nonprofits only
pGEX-4T3-ZO-2-1st-PDZ

Plasmid

#27424

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

March 31, 2011

Availability

Academic Institutions and Nonprofits only
pEBB-GFP-hBEN-deltaNLS3

Plasmid

#22158

Depositor

Ananda Roy

Article

Tussie-Luna et al Proc Natl Acad Sci U S A. 2001 Jul 3. 98

Insert

hBEN (GTF2IRD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Deletion of amino acids 883-889

Promoter

Available since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only
pcDNA4/HisMaxB-YAP2-deltaC

Plasmid

#21124

Depositor

Marius Sudol

Article

Tsutomu Oka & Marius et al

Insert

Yes-kinase associated protein (YAP1 Human)

Use

Tags

His

Expression

Mammalian

Mutation

5 Carboxy-terminal amino acids -FLTWL are deleted

Promoter

Available since

July 1, 2009

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1CH2A

Plasmid

#13473

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2A (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 1-46

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pWay21-MKP-1CH2B

Plasmid

#13474

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2B (Dusp1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 47-136

Promoter

Available since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pCAGGS-Twin-strep-TEV-DGKδ2

Plasmid

#223720

Purpose

Express human diacylglycerol kinase delta 2 protein (N-terminal Tobacco Etch Virus (TEV) protease cleavable Twin-Strep-fusion protein (ENLYFQGS-WSHPQFEK-(GGGS)2-GGSA-WSHPQFEK)) in mammalian cell

Depositor

Chiaki Murakami

Insert

DGKD (DGKD Human)

Use

Tags

TEV cleavable site and Twin-Strep-tag

Expression

Mammalian

Mutation

deleted start codon

Promoter

CAG and chicken β-actin promoter

Available since

Jan. 8, 2025

Availability

Academic Institutions and Nonprofits only

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