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MMK

Plasmid

#64121

Purpose

a modified episomal (EBNA1/OriP) vector expressing human Myc and KLF4 genes

Depositor

Linzhao Cheng

Article

Chou et al Stem Cells Transl Med. 2015 Mar 5. pii:

Insert

MYC-2A-KLF4 (MYC, KLF4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TSC2

Plasmid

#15478

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

TSC2 shRNA (TSC2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pGL3-mArg1 promoter/enhancer -31/-3810

Plasmid

#34571

Depositor

Peter Murray

Article

Pauleau et al J Immunol. 2004 Jun 15;172(12):7565-73.

Insert

Arg1 promoter/enhancer -31/-3810 (Arg1 Mouse)

Use

Luciferase

Tags

Firefly Luciferase

Expression

Mutation

Promoter

Available since

Jan. 17, 2012

Availability

Academic Institutions and Nonprofits only

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pCMVHA EED wt

Plasmid

#24231

Purpose

Mammalian expression of human EED with HA tag

Depositor

Kristian Helin

Article

Bracken et al EMBO J. 2003 Oct 15. 22(20):5323-35.

Insert

EED (EED Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2011

Availability

Academic Institutions and Nonprofits only

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pUbC-OsTIR1-myc-IRES-scFv-sfGFP

Plasmid

#84563

Purpose

Bicistronic vector for expression of O. sativa E3 ubiquitin ligase and single chain variable fragment of GCN4 antibody fused to superfolder GFP

Depositor

Robert Singer

Article

Wu et al Science. 2016 Jun 17;352(6292):1430-5. d

Insert

Transport inhibitor response 1 (E3 ligase domain), IRES, single chain variable fragment-superfolder GFP

Use

Lentiviral

Tags

myc tag (9x)

Expression

Mutation

Promoter

Available since

Dec. 20, 2016

Availability

Academic Institutions and Nonprofits only

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1478 pcDNA3 flag HA 14-3-3 beta

Plasmid

#8999

Depositor

William Sellers

Insert

14-3-3 beta (YWHAB Human)

Use

Tags

HA and flag

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
AnkyrinB-2XHA

Plasmid

#31057

Depositor

Vann Bennett

Article

Ayalon et al J Biol Chem. 2011 Mar 4. 286(9):7370-8.

Insert

Ankyrin B (ANK2 Human)

Use

Tags

2XHA

Expression

Mammalian

Mutation

Promoter

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 iTEV

Plasmid

#64277

Purpose

Expresses infrared fluorescent tobacco etch virus protease sensor (iTEV)

Depositor

Xiaokun Shu

Article

To et al Proc Natl Acad Sci U S A. 2015 Mar 17;11

Insert

Infrared fluorescent tobacco etch virus protease sensor (iTEV)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pWJ66

Plasmid

#46570

Purpose

Same as pdCas9, but with the dCas9-w fusion (w is fused at the C-terminal end of dCas9)

Depositor

Luciano Marraffini

Article

Bikard et al Nucleic Acids Res. 2013 Jun 12.

Inserts

tracrRNA
dcas9-w
CRISPR array

Use

CRISPR

Tags

rpoZ

Expression

Bacterial

Mutation

D10A, H840A

Promoter

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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mScarlet-I-LaminB

Plasmid

#98831

Purpose

In vivo visualization of the nuclear envelope (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv 160374

Insert

CytERM

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

*(see below)

Promoter

CMV

Available since

Nov. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pLifeAct_mScarlet-i_N1

Plasmid

#85056

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

LifeAct

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pSG5-ABL

Plasmid

#31284

Depositor

Nora Heisterkamp

Article

Yi et al Int J Mol Med. 2008 Oct . 22(4):453-8.

Insert

V-abl Abelson murine leukemia viral oncogene homolog 1 (ABL1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pMIG FLAG B55 alpha

Plasmid

#13804

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

PP2A B55 alpha Flag (PPP2R2A Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2007

Availability

Academic Institutions and Nonprofits only
Tagged-Cap

Plasmid

#35572

Depositor

Marian Waterman

Article

Tsai et al Mol Cell Proteomics. 2011 Apr;10(4):M110

Type

Empty backbone

Use

Luciferase

Tags

Ms2 Stem Loop

Expression

Mammalian

Mutation

Promoter

SV40

Available since

April 3, 2012

Availability

Academic Institutions and Nonprofits only

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mCherry-Sequestosome1-N-18

Plasmid

#55132

Purpose

Localization: Membrane Scaffold, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Sequestosome1 (SQSTM1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-flag-YTHDF2

Plasmid

#52300

Purpose

full length YTHDF2 with N-terminal flag tag for mammalian cell expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

Promoter

T7

Available since

April 14, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-K-CAAX

Plasmid

#41644

Purpose

Expresses wild-type Dronpa fused to the membrane-anchoring K-Ras C-terminal farnesylation motif (CAAX box)

Depositor

Michael Lin

Article

Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi

Insert

Dronpa145K-CAAX

Use

Tags

CAAX box

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 13, 2012

Availability

Academic Institutions and Nonprofits only

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p2xFLAGhYAP1-S127A

Plasmid

#17790

Depositor

Marius Sudol

Article

Komuro et al J Biol Chem. 2003 Aug 29. 278(35):33334-

Insert

Yes-kinase associated protein mutant S127A (YAP1 Human)

Use

Tags

2xFlag

Expression

Mammalian

Mutation

Serine 127 to Alanine

Promoter

Available since

May 9, 2008

Availability

Academic Institutions and Nonprofits only

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pLenti PGK Neo DEST (w531-1)

Plasmid

#19067

Purpose

3rd gen lentiviral Gateway destination vector, expression, PGK promoter, Neo

Depositor

Paul Kaufman, Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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Fv-hCaspase 8-2A-GFP

Plasmid

#82712

Purpose

express human caspase 8 in mammalian cells

Depositor

Douglas Green

Article

Oberst et al J Biol Chem. 2010 May 28;285(22):16632-4

Insert

Fv-Caspase 8-2A-GFP (CASP8 Human)

Use

Retroviral

Tags

FKBP dimerization domain

Expression

Mammalian

Mutation

deleted amino acids 1-215 of caspase 8

Promoter

LTR

Available since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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