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Plasmid#105517PurposeExpression of mCherry-tagged mouse Trim21 with a deleted RING-box in mammalian cellsDepositorInsertTRIM21 (Trim21 Mouse)
UseTagsmCherryExpressionMammalianMutationdeletion of RING domain (aa124-462)PromoterCMVAvailable sinceMarch 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRK5-ATP6V1D-HAGST
Plasmid#87932PurposeC-term HA-GST tagged overexpressionDepositorInsertATP6V1D (ATP6V1D Human)
UseTagsHA-GSTExpressionMammalianMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSL1749 (pDonor, R*MmeI)
Plasmid#160733PurposeMini-transposon donor plasmid for V. cholerae CAST system. Mini-transposon has MmeI site in R end for Tn-seq. pUC19 vector backbone.DepositorInsertVchCAST donor DNA
UseTagsExpressionBacterialMutationPromoterN/AAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOO2-GW
Plasmid#135097PurposeGateway vector for expression of proteins in Xenopus oocytesDepositorTypeEmpty backboneUseBacterial expression vector for generating sp6 tr…TagsExpressionMutationPromoterSP6Available sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
LDB-3-p65
Plasmid#158984PurposeExpresses LDB-3 fused with a C-terminal p65 transcriptional activation domain in mammalian cellsDepositorInsertLDB-3-p65
UseLentiviralTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.4_17G6-1 light chain
Plasmid#198250Purposerecombinant expression of light chain of monoclonal anti-AMP antibody 17G6-1DepositorInsertlight chain of monoclonal anti-AMP antibody 17G6-1 (mouse kappa)
UseTagsIL10 signal peptideExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCherry.90 beta
Plasmid#108223PurposeMammalian expression vector for mCherry fusion to human Hsp90 betaDepositorInsertmCherry-Hsp90b (HSP90AB1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS3-Syn21-V5-mCherry-P10
Plasmid#104617PurposeExpresses V5 tagged mCherry upon binding of TALE3 to VAS3DepositorInsertSyn21-V5-mCherry-P10-MhcSA
UseTagsV5ExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCl-HA-YAP
Plasmid#27007DepositorInsertYAP (YAP1 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceFeb. 4, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEG BacMam C term GFP His8
Plasmid#160681PurposeVector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target proteinDepositorTypeEmpty backboneUseTagsHis8 GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
eGFP-KIF5A
Plasmid#172201PurposeExpresses KIF5A tagged with eGFP in mammalian cellsDepositorInsertKIF5A (Kif5a Mouse)
UseTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Endosomal Escape Control
Plasmid#186552PurposeNegative Control confirming the need for a CPP peptide in mediating internalizationDepositorInsert6H-ETA-GFP
UseTags6xHis and EGFPExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Pet15b-Internalization and Endosomal Escape
Plasmid#186551PurposeInternalization and Endosomal Escape to effectively route a protein cargo to the cytosol of cellsDepositorInsert10R-8His-ETA-EGFP
UseTags8xHis and EGFPExpressionBacterialMutationPromoterT7Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMito-mCherry-FRB_IRES_FKBP(III)-GBPen
Plasmid#128268PurposeBicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 3x FKBP-GBPenhancer (GFP Nanobody with 3 FKBP domains).DepositorInsertsMitoTrap
FKBP(III)-GBPen
UseTagsExpressionMammalianMutationPromoterCMV and IRESAvailable sinceAug. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV6-AN-HA_WT POLK
Plasmid#201584PurposeExpresses human DNA polymerase kappa with an N-terminal HA tag in mammalian cellsDepositorInsertPolymerase kappa (POLK Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF1a_SOX3_P2A_Hygro_Barcode
Plasmid#120481PurposeBarcoded lentiviral vector to express SOX3 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertSOX3 (SOX3 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI HA NEDD4L DD
Plasmid#27001DepositorInsertNEDD4L (NEDD4L Human)
UseTagsHAExpressionMammalianMutationC962APromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pKH162_FLAG-APEX2-YIPF3 LIR mut
Plasmid#214981PurposeFlag-APEX2 tagged LIR mutant YIPF3DepositorInsertFLAG-APEX2-YIPF3_mut (YIPF3 Human)
UseLentiviralTagsExpressionMutationLIR mutantPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
SaCas9_sgRNA_expression_in_pBluescript
Plasmid#122090PurposeU6 driven SaCas9 sgRNA expression vector for cloning own guidesDepositorInsertSaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceApril 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4 mIL-17 2kb promoter
Plasmid#20124DepositorInsertmIL-17 (Il17a Mouse)
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceJan. 16, 2009AvailabilityAcademic Institutions and Nonprofits only