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PurposeMammalian expression vector for mCherry fusion to human Hsp90 beta
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 108223 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepmCherry-C21
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry-Hsp90b
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SpeciesH. sapiens (human)
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Entrez GeneHSP90AB1 (a.k.a. D6S182, HSP84, HSP90B, HSPC2, HSPCB)
- Promoter CMV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCherry.90 beta was a gift from Didier Picard (Addgene plasmid # 108223 ; http://n2t.net/addgene:108223 ; RRID:Addgene_108223) -
For your References section:
2-color photobleaching experiments reveal distinct intracellular dynamics of two components of the Hsp90 complex. Picard D, Suslova E, Briand PA. Exp Cell Res. 2006 Nov 15. 312(19):3949-58. 10.1016/j.yexcr.2006.08.026 PubMed 17010336