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Addgene

Dominant negative Hsp90 beta
(Plasmid #22480)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22480 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.0
  • Backbone size w/o insert (bp) 5400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Dominant negative Hsp90
  • Alt name
    Hsp90beta D88N
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2700
  • Entrez Gene
    HSP90AB1 (a.k.a. D6S182, HSP84, HSP90B, HSPC2, HSPCB)
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (destroyed during cloning)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Dominant negative Hsp90 beta was a gift from William Sessa (Addgene plasmid # 22480 ; http://n2t.net/addgene:22480 ; RRID:Addgene_22480)
  • For your References section:

    Dominant-negative Hsp90 reduces VEGF-stimulated nitric oxide release and migration in endothelial cells. Miao RQ, Fontana J, Fulton D, Lin MI, Harrison KD, Sessa WC. Arterioscler Thromb Vasc Biol. 2008 Jan . 28(1):105-11. 10.1161/ATVBAHA.107.155499 PubMed 17975117