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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22480 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepcDNA3.0
- Backbone size w/o insert (bp) 5400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDominant negative Hsp90
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Alt nameHsp90beta D88N
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SpeciesH. sapiens (human)
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Insert Size (bp)2700
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Entrez GeneHSP90AB1 (a.k.a. D6S182, HSP84, HSP90B, HSPC2, HSPCB)
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Tag
/ Fusion Protein
- HA (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site NotI (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Dominant negative Hsp90 beta was a gift from William Sessa (Addgene plasmid # 22480 ; http://n2t.net/addgene:22480 ; RRID:Addgene_22480) -
For your References section:
Dominant-negative Hsp90 reduces VEGF-stimulated nitric oxide release and migration in endothelial cells. Miao RQ, Fontana J, Fulton D, Lin MI, Harrison KD, Sessa WC. Arterioscler Thromb Vasc Biol. 2008 Jan . 28(1):105-11. 10.1161/ATVBAHA.107.155499 PubMed 17975117