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Plasmid#21647DepositorInsertCyclin D2 long-mut-miR15/16 (CCND2 Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationMutant miR15/16 microRNA binding sitesPromoterAvailable sinceJuly 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
M47 MBP-TEV-Chibby
Plasmid#17195DepositorInsertChibby (CBY1 Human)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceOct. 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-Nup35-mut
Plasmid#87341PurposeTo express human Nup35 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP35 (NUP35 Human)
UseTagsEGFPExpressionMammalianMutationshRNA resistant changesPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0112
Plasmid#117548PurposeLevel 1 Golden Gate Cassette: xCas9 3.7 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + Sp-xCas9 3.7 (no stop codon) (pEPOR0SP0011) + YFP (pICSL50005) + Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
CD4_LD 0_CS G_tTA-BFP
Plasmid#58869PurposeMESA target chain with CD4 ectodomain, no linker domain, G cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with CD4 ectodomain, cd28 transmembrane domain, wild type TEV cleavage sequence
UseAAVTagsEBFPExpressionMammalianMutationPromoterCMVAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 humanN-Flag SadBS
Plasmid#66881PurposeMammalian expression vector for expressing N-terminally Flag-tagged short isoform of SadBDepositorInsertSadB (BRSK1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pWZL N-Myc-hPOT1_deltaOB
Plasmid#166408Purposeexpress hPOT1 ΔOB in mammalian cellsDepositorInsertPOT1 ΔOB (POT1 Human)
UseTagsmycExpressionMammalianMutationΔOBPromoterAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL N-Myc-hPOT1_IRES_hygro
Plasmid#166406Purposeexpress hPOT1 in mammalian cellsDepositorInserthPOT1 (POT1 Human)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1X-Nup88-mut
Plasmid#87337PurposeTo express human Nup88 in mammalian cells. It contains synonymous mutations that are not targeted by shRNADepositorInsertNUP88 (NUP88 Human)
UseTagsEGFPExpressionMammalianMutationshRNA resistant changesPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-Elafin
Plasmid#18099DepositorInsertElafin (PI3 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 11, 2008AvailabilityAcademic Institutions and Nonprofits only -
pICSL11057
Plasmid#68253PurposeLevel 1 Golden Gate Cassette: sgRNA cassette targeting PM19_1 in barleyDepositorInsertPromote:TaU6+sgRNA HvPM19_1
UseSynthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSKB3-SaGGR-I206F-L377H (JBEI-7202)
Plasmid#110152PurposepSKB3 with TEV-cleavable, N-terminal His-tagged SaGGR-I206F-L377H mutant geneDepositorInsertGGR
UseTagsTEV-cleavable His-tagExpressionBacterialMutationSaGGR-I206F-L377H mutant genePromoterT7Available sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RLuc8C-FHA1-NES
Plasmid#138210PurposeEncodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.DepositorInsertRLuc8C-FHA1-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKAsub-RLuc8N-NES
Plasmid#138211PurposeEncodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKAsub-RLuc8N-NES
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPOR1CB0113
Plasmid#117552PurposeLevel 1 Golden Gate Cassette: eSaCas9 expression cassette for dicotyledonous plantsDepositorInsert2x35S+5'UTR OMEGA (pICH51288) + eSaCas9 (no stop codon) (pEPOR0SP0012) + YFP (pICSL50005) + Nos (pICH41421)
UseTagsExpressionPlantMutationPromoter2x35S+5'UTR OMEGA (pICH51288)Available sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
OVA-miSFIT-19-G
Plasmid#124676PurposeTuning expression of cytosolic Ovalbumin coupled to EGFPDepositorInsertCyto-Ovalbumin
UseTagsExpressionMammalianMutationTruncated OVA for cytoplasmic expressionPromoterminimal CMVAvailable sinceMay 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
PcDNA-DEST53-CypB-Nucl N-term GFP
Plasmid#36123DepositorInsertcyclophilin B (PPIB Human, contains CypB AA 33-216)
UseTagsGFPExpressionMammalianMutationA212SPromoterCMVAvailable sinceNov. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pOCC215
Plasmid#118879Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal Dendra2, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-Dendra2-3C-HIS6-stop-HindIII cassette
UseTagsDendra2, HIS6, cleavable with 3C proteaseExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV-phsyn-FLEX-KASH-EGFP-U6-ShGmeb1
Plasmid#129706PurposeExpress cre-dependent KASH-EGFP and Gmeb1 shRNADepositorInsertKASH-EGFP
UseAAVTagsEGFPExpressionMammalianMutationPromoterU6Available sinceAug. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2Flag CMV2-ZO-2-1st-PDZ-mut
Plasmid#27416DepositorInsertZonula Occludens 2 (TJP2 Human)
UseTags2xFlagExpressionMammalianMutationK38E, F44H, I46E and V48EPromoterAvailable sinceApril 12, 2011AvailabilityAcademic Institutions and Nonprofits only