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RN3P-KDM5B-FLAG

Plasmid

#86398

Purpose

FLAG-tagged mouse KDM5B cDNA clone for mammalian cell expression

Depositor

Wei Xie

Article

Zhang et al Nature. 2016 Sep 14;537(7621):553-557. d

Insert

KDM5B (Kdm5b Mouse)

Use

Tags

3x-FLAG

Expression

Mammalian

Mutation

Promoter

Lac

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Cdc42(Q61L)

Plasmid

#12600

Depositor

Klaus Hahn

Article

Nalbant et al Science. 2004 Sep 10. 305(5690):1615-9.

Insert

EGFP-Cdc42 (Q61L) (CDC42 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q61L

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pDuet TDP43 WT

Plasmid

#27462

Depositor

Aaron Gitler

Article

Johnson et al J Biol Chem. 2009 Jul 24. 284(30):20329-

Insert

TDP-43 (TARDBP Human)

Use

Coexpression

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only

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pWZL Hygro

Plasmid

#18750

Depositor

Scott Lowe

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 3, 2008

Availability

Academic Institutions and Nonprofits only
pGWB402

Plasmid

#74796

Purpose

Gateway cloning compatible binary vector for expression of gene by CaMV35S promoter.

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV35S

Available since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

Plasmid

#84467

Purpose

Empty pAAV-CRAM plasmid w. multiple cloning site (MCS)

Depositor

Yingxi Lin

Article

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Type

Empty backbone

Use

AAV

Tags

Expression

Mutation

Promoter

pRAM and TRE

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-Parkin

Plasmid

#17613

Depositor

Ted Dawson

Article

Zhang et al Proc Natl Acad Sci U S A 2000 Nov 21;97(

Insert

Parkin (PRKN Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 3, 2008

Availability

Academic Institutions and Nonprofits only

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pBABE puro mouse IRS-1 myc

Plasmid

#11374

Depositor

Ronald Kahn

Article

Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

Insert

IRS-1 (Irs1 Mouse)

Use

Retroviral

Tags

myc

Expression

Mammalian

Mutation

Promoter

Available since

April 20, 2006

Availability

Academic Institutions and Nonprofits only

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Rab5a-pmCherryC1

Plasmid

#27679

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

Rab5a (Rab5a Mouse)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

May 4, 2011

Availability

Academic Institutions and Nonprofits only

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mIFP-N1

Plasmid

#54620

Purpose

Localization: N1 Cloning Vector, Excitation: 683, Emission: 704

Depositor

Michael Davidson, Xiaokun Shu

Article

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Type

Empty backbone

Use

Tags

mIFP

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA-D1ER

Plasmid

#36325

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1ER second generation cameleon (calcium sensor) targeted to ER

Use

Tags

KDEL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pRK5-HA GST RagD 121L

Plasmid

#19309

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding D (RRAGD Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Q121L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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GolgiCFP

Plasmid

#14873

Depositor

Alexandra Newton

Article

Gallegos et al J Biol Chem. 2006 Oct 13. 281(41):30947-

Insert

CFP

Use

Tags

eNOS(1-35)

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 9, 2007

Availability

Academic Institutions and Nonprofits only

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mEmerald-LaminB1-10

Plasmid

#54140

Purpose

Localization: Nuclear Envelope, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

LaminB1 (LMNB1 Human)

Use

Tags

mEmerald

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pLVX-EF1alpha-SARS-CoV-2-orf8-2xStrep-IRES-Puro

Plasmid

#141390

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf8 (ORF8 SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1/hChR2-EYFP

Plasmid

#20939

Purpose

Mammalian expression of humanized ChR2 fused to EYFP

Depositor

Karl Deisseroth

Article

Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.

Insert

channelrhodopsin-2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pAAV-nEF Con/Fon hChR2(H134R)-EYFP

Plasmid

#55644

Purpose

Cre-on/Flp-on ChR2-EYFP under the short Ef1a promoter

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Insert

ChR2(H134R)-EYFP

Use

AAV; Cre on/flp on chr2-eyfp

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

nEF

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pMK344 (Hygro-P2A-mAID)

Plasmid

#121178

Purpose

N-terminal tagging

Depositor

Masato Kanemaki

Article

Yesbolatova et al Methods. 2019 Apr 23. pii: S1046-2023(18

Insert

Hygro-P2A-mAID

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pSimple-miR302/367

Plasmid

#98748

Purpose

Episomal reprogramming

Depositor

Sara Howden, James Thomson

Article

Howden et al Stem Cell Reports. 2015 Dec 8;5(6):1109-

Insert

miR302/367 (miRNA cluster) (MIR302A, MIR367 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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PX855

Plasmid

#62887

Purpose

N-term SpCas9 piece of inducible transcriptional activator (dCas9(N)-FRB-2xNES)

Depositor

Feng Zhang

Article

Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038

Insert

SpCas9 (aa 2-535)

Use

CRISPR

Tags

FRB and NES PTK2

Expression

Mammalian

Mutation

Aspartic acide 10 to Alanine (D10A)

Promoter

CBh

Available since

March 5, 2015

Availability

Academic Institutions and Nonprofits only

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