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pCSIIneo-TRE-hPhyB621- mCherry-HRasCT

Plasmid

#139482

Purpose

Expresses PhyB621-mCherry-HRasCT in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

PhyB621 (PHYB Mustard Weed)

Use

Lentiviral

Tags

mCherry-HRasCT

Expression

Mammalian

Mutation

G229L and deletion of aa Y at position 235 in mch…

Promoter

Tet responsible element

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-A

Plasmid

#138682

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-C

Plasmid

#138683

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Promoter

hU6

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pBlue-hFX-HA

Plasmid

#14987

Depositor

Michael Ristow

Article

Shoichet et al Hum Mol Genet. 2002 Apr 1. 11(7):815-21.

Insert

human frataxin cDNA (FXN Human)

Tags

AU1 and HA

Expression

Bacterial

Available Since

Aug. 17, 2007

Availability

Academic Institutions and Nonprofits only
MigR1-Myc-Nterm TRIB2

Plasmid

#131153

Purpose

Retroviral expression of N terminus of mouse TRIB2

Depositor

Karen Keeshan, Warren Pear

Article

Keeshan et al Cancer Cell. 2006 Nov;10(5):401-11. doi:

Insert

Trib2 (Trib2 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

contains amino acids 1-145

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pUDR220

Plasmid

#113873

Purpose

expression of Cas9 programming sgRNA8 and sgRNA7 targetting HXT10 and HXT9-11-12 respectively

Depositor

Jean-Marc Daran, Jack Pronk

Article

Wijsman et al FEMS Yeast Res. 2019 Jan 1;19(1). pii: 5

Insert

sgRNA8-HXT10 sgRNA7-HXT9-11-12

Use

CRISPR

Expression

Yeast

Available Since

Sept. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCOG75-Sem1 Sc

Plasmid

#83233

Purpose

pCDF-His6-MBP-Sem1 Sc

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

SEM 1 (SEM1 Budding Yeast)

Tags

His-mbp

Expression

Bacterial

Available Since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-AuroraB L154G/H250Y

Plasmid

#108490

Purpose

expression of vsv-AuroraB Analog sensitive (L154G/H250Y)

Depositor

Susanne Lens

Article

Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

Insert

AuroraB (AURKB Human)

Tags

VSV

Expression

Mammalian

Mutation

Analog sensitive L154G/H250Y

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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RSV/mGATA3 (sense)

Plasmid

#83816

Purpose

Mammalian expression of mouse GATA3

Depositor

Douglas Engel

Article

Ko et al Mol Cell Biol. 1991 May . 11(5):2778-84.

Insert

GATA3 (Gata3 Mouse)

Expression

Mammalian

Promoter

RSV

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only
STARR-seq luciferase validation vector_mP_CMV

Plasmid

#99313

Purpose

Luciferase validation vector with CMV enhancer inserted downstream of the reporter gene. The candidate's enhancer activity is reflected by luciferase activity

Depositor

Alexander Stark

Article

Muerdter et al Nat Methods. 2017 Dec 11. pii: nmeth.453

Insert

CMV enhancer

Use

Luciferase; Starr-seq luciferase validation vector

Expression

Mammalian

Available Since

Sept. 26, 2017

Availability

Academic Institutions and Nonprofits only

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Luc-Nanog-3'UTRmut

Plasmid

#63894

Purpose

Luciferase reporter of mouse Nanog 3'UTR with the miR-34 target site mutated

Depositor

Lin He

Article

Choi et al Nat Cell Biol. 2011 Oct 23;13(11):1353-6

Insert

Nanog homeobox (Nanog Mouse)

Expression

Mammalian

Available Since

June 19, 2015

Availability

Academic Institutions and Nonprofits only
Tip alpha K102A/K104A (21-192)

Plasmid

#23159

Depositor

SeWon Suh

Article

Jang et al J Mol Biol. 2009 Sep 11. 392(1):191-7.

Insert

TNF-α-inducing protein

Tags

His

Expression

Bacterial

Mutation

changed lysine 102 and lysine 104 to alanine, and…

Available Since

April 16, 2010

Availability

Academic Institutions and Nonprofits only
pYPQ133B-TLS-CsALSgR1.2

Plasmid

#245878

Purpose

Golden gate entry vector carrying the 2nd gRNA for base editing in Carrizo citrange ALS gene along with TLS mobile RNA sequence

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

CsALSgR1.2

Use

CRISPR; Golden gate entry vector

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pYPQ132B-CsALSgR1.1

Plasmid

#245875

Purpose

Golden gate entry vector carrying the 1st gRNA for base editing in Carrizo citrange ALS gene

Depositor

Yiping Qi

Article

Rocha et al Plant Cell Rep. 2025 Oct 22;44(11):244.

Insert

CsALS_gRNA1.1

Use

CRISPR; Golden gate entry vector to expressing t…

Expression

Plant

Promoter

AtU3

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-RVdG-MCP:oScarlet:KASH-6xMBS

Plasmid

#231011

Purpose

CVS N2c RVdG genome plasmid. Utilizes MS2 tagging to deliver the MCP:oScarlet:KASH transcript to the outer nuclear membrane. Insert high complexity barcode between SacII RE sites distal to 6xMBS site.

Depositor

Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Insert

MCP-oScarlet-KASH-6xMBS-SacIIMCS

Use

Neurotropic virus

Tags

KASH and MCP

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-RVdG-MCP:oScarlet-KASH_NoMBS

Plasmid

#231014

Purpose

CVS N2c RVdG genome plasmid, utilized to generate negative control virus to benchmark the utility of MS2 tagging for capture of barcodes using snRNA-seq.

Depositor

Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Insert

MCP-oScarlet-KASH

Use

Neurotropic virus

Tags

KASH and MCP

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-Alpha 49

Plasmid

#244910

Purpose

Rep/Cap plasmid for producing variant AAV-hCA4-Alpha 49

Depositor

Viviana Gradinaru

Article

Lin et al Cell Rep. 2025 Oct 31;44(11):116419. doi

Insert

Synthetic construct producing AAV REP/CAP

Use

AAV

Expression

Mammalian

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-hCA4-IV68

Plasmid

#244907

Purpose

Rep/Cap plasmid for producing variant AAV-hCA4-IV68

Depositor

Viviana Gradinaru

Article

Lin et al Cell Rep. 2025 Oct 31;44(11):116419. doi

Insert

Synthetic construct producing AAV REP/CAP

Use

AAV

Expression

Mammalian

Available Since

Nov. 17, 2025

Availability

Academic Institutions and Nonprofits only

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pBK1837

Plasmid

#239840

Purpose

7. BK1837- pLV-CMV-SNCAintron1-AlphaSyn-d2EGFP-P2A-NLuc-SV40-Puro-WPRE

Depositor

Boris Kantor

Article

Kantor et al Mol Ther. 2018 Nov 7;26(11):2638-2649. d

Inserts

pLV-CMV-SNCAintron1-AlphaSyn-d2EGFP-P2A-NLuc-SV40-Puro-WPRE (pCL35)
pLV-CMV-SNCAintron1-AlphaSyn-d2EGFP-P2A-NLuc-SV40-Puro-WPRE (pCL35)

Use

Lentiviral

Promoter

CMV

Available Since

Nov. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pYO829

Plasmid

#242492

Purpose

Expression of ScAtg38 Δ(182 to 209)-3xFLAG under its own promoter

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

ATG38 (YLR211C Budding Yeast)

Tags

3xFLAG

Expression

Yeast

Mutation

deletion of aa 182 to 209

Available Since

Oct. 27, 2025

Availability

Academic Institutions and Nonprofits only

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